Development of novel nucleic acid medicine artificial bone using bioprocessed NF-kB decoy

• Project/Area Number: 23390443
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: KUBO TAKAYASU 広島大学, 大学病院, 講師 (60240876)
• Co-Investigator(Kenkyū-buntansha): HIASA Kyo 広島大学, 大学病院, 助教 (60304432) ; DOI Kazuya 広島大学, 大学院・医歯薬保健学研究院, 助教 (80444686) ; AKAGAWA Yasumasa 奥羽大学, 歯学部, 教授 (00127599)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2011: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
• Keywords: NF-κBデコイ / 連通多孔性アパタイト / ESCA / 骨髄由来破骨前駆細胞 / TRAP / RANKL / 破骨細胞 / インプラントGBR / 骨芽細胞 / 骨髄由来破骨前駆細動

Research Abstract

This study aimed to develop the newly useful artificial bone material combined the NF-kB decoy which is known as nucleic acid medicine with the polyphosphoric acid reported that is restrained the osteoclasts and promoted the activity of the osteoblasts. It was clarified that the NF-kB decoy was restrained strongly the activity of the osteoclasts in vitro by the TRAP staining and the measurement of TRAP positive cells. It was clarified also that the polyphoshoric acid was promoted the activity of the osteoblast in vitro. Furthermore, it was confirmed that the artificial bone with the polyphosphoric acid and the NF-kB decoy was promoted the bone formation in vivo. In the results of this study, we were able to acquire the significant knowledge about the development of the newly artificial bone which is influenced both of the osteoclasts and the osteoblasts.

Research Products

• [Journal Article] Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration : an animal study (2014)
  • Author(s): Doi K, Kubo T, Takeshita R, Kajihara S, Kato S, Kawazoe Y, Shiba T, Akagawa Y
  • Journal Title: Dent Mater J Volume: 33 Pages: 179-86
  • NAID: 130003394967
  • Peer Reviewed
• [Journal Article] Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: an animal study. (2014)
  • Author(s): 7. Doi K, Kubo T, Takeshita R, Kajihara S, Kato S, Kawazoe Y, Shiba T, Akagawa Y
  • Journal Title: Dent Mater J Volume: 33 Pages: 179-186
  • NAID: 130003394967
  • Peer Reviewed
• [Journal Article] Inorganic Polyphosphate Suppresses Lipopolysaccharide-Induced Inducible Nitric Oxide Synthase (iNOS) Expression in Macrophages (2013)
  • Author(s): Harada K, Shiba T, Doi K, Morita K, Kubo T, Makihara Y, Piattelli A, Akagawa Y
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Polyphosphate-mediated inhibition of tartrate-resistant Acid phosphatase and suppression of bone resorption of osteoclasts (2013)
  • Author(s): Harada K, Itoh H, Kawazoe Y, Miyazaki S, Doi K, Kubo T, Akagawa Y, Shiba T
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Inorganic Polyphosphate Suppresses Lipopolysaccharide-Induced Inducible Nitric Oxide Synthase (iNOS) Expression in Macrophages. (2013)
  • Author(s): 2. Harada K, Shiba T, Doi K, Morita K, Kubo T, Makihara Y, Piattelli A, Akagawa Y
  • Journal Title: PLoS One Volume: 8(9)
  • Peer Reviewed
• [Journal Article] Polyphosphate-mediated inhibition of tartrate-resistant Acid phosphatase and suppression of bone resorption of osteoclasts. (2013)
  • Author(s): 3. Harada K., Itoh H., Kawazoe Y., Miyazaki S., Doi K., Kubo T., Akagawa Y, Shiba T
  • Journal Title: PLoS One Volume: 8(11)
  • Peer Reviewed
• [Journal Article] ポリリン酸吸着人工骨の開発 (2013)
  • Author(s): 土井一矢
  • Journal Title: 日本補綴歯科学会誌 Volume: 5(4) Pages: 417-419
  • NAID: 10031202890
  • Peer Reviewed
• [Presentation] Histological and mechanical evaluation of the implants placed in the reconstructed bone by interconnected porous calcium hydroxyapatite (2013)
  • Author(s): Makihara Y, Doi K, Morita K, Kajihara S, Kubo T, Akagawa Y
  • Organizer: The 15th Biennial Meeting of International College of Prosthodontists
  • Place of Presentation: Torino, Italy
  • Year and Date: 2013-09-19
• [Presentation] 連通多孔性ハイドロキシアパタイトによる骨再建部に対するインプラント埋入 : 組織学的および力学的評価 (2013)
  • Author(s): 牧原勇介, 土井一矢, 梶原志穂, 久保隆靖, 赤川安正
  • Organizer: 日本口腔インプラント学会
  • Place of Presentation: 博多
  • Year and Date: 2013-09-13
• [Presentation] 補綴治療の向上に貢献する「十分に検証された既存の技術をハイブリッドさせたバイオマテリアルの創生」 (2013)
  • Author(s): 土井一矢
  • Organizer: 公益社団法人日本補綴歯科学会 第122回学術大会 シンポジウム
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2013-05-18
• [Presentation] Development of composite of poly(P) and bFGF with interconnected porous calcium hydroxyapatite (2012)
  • Author(s): Morita K, Doi K, Kubo T, Kajihara S, Takeshita R, Quan Y, Ping, G, Akagawa, Y
  • Organizer: 2012 Sino-Japan Dental Conference
  • Place of Presentation: Chengdu, China
  • Year and Date: 2012-04-27
• [Presentation] 補綴治療の向上に貢献する「十分に検証された既存の技術をハイブリッドさせたバイオマテリアルの創生」
  • Author(s): 土井一矢
  • Organizer: 日本補綴歯科学会第122回学術大会
  • Place of Presentation: 福岡
• [Presentation] 連通多孔性ハイドロキシアパタイトによる骨再建部に対するインプラント埋入：組織学的および力学的評価
  • Author(s): 牧原勇介, 土井一矢, 梶原志穂, 久保隆靖, 赤川安正
  • Organizer: 第43回日本口腔インプラント学会総会･学術大会
  • Place of Presentation: 福岡
• [Presentation] Histological and mechanical evaluation of the implants placed in the reconstructed bone by interconnected porous calcium hydroxyapatite
  • Author(s): Makihara Y, Doi K, Morita K, Kajihara S, Kubo T, Akagawa Y
  • Organizer: International College of Prosthodontists
  • Place of Presentation: Torino