Improvement of the replacement of carbonate apatite to bone by the regulation of porous structure

• Project/Area Number: 23390444
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: Kyushu University
• Principal Investigator: ISHIKAWA Kunio 九州大学, 歯学研究科(研究院), 教授 (90202952)
• Co-Investigator(Renkei-kenkyūsha): TSURU Kanji 九州大学, 大学院歯学研究院, 准教授 (50314654) ; MATSUYA Shigeki 福岡歯科大学, 歯学研究科, 教授 (00108755) ; MIYAMOTO Youji 徳島大学, ヘルスバイオサイエンス研究部 (20200214) ; MARUTA Michito 福岡歯科大学, 歯学研究科, 講師 (40507802)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: 炭酸アパタイト / 海綿骨 / 高温分解 / 溶解析出 / 前駆体 / 海面骨

Outline of Final Research Achievements

The objective of this study was to evaluate the feasibility of fabricating artificial bone substitute from xenograft based on the compositional transformation to α-tricalcium phosphate followed by compositional transformation to carbonate apatite maintaining its macroscopic structure. Heat treatment at 900°C result in complete burning out of the organic substrate indicating antigen-antibody reaction will be completely eliminated. Then, the bone is further heated at 1500°C for compositional transformation to α-tricalcium phosphate which has higher reactivity and slightly dissolves in water. Then the bone with α-tricalcium phosphate composition was hydrothermally treated in 4 mol/L ammonium carbonate at 250°C. Based on the dissolution-precipitation reaction, composition of the bone with α-tricalcium phosphate composition transferred to carbonate apatite maintaining its macroscopic structure.