| Project/Area Number |
23390456
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | National Center for Geriatrics and Gerontology |
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Principal Investigator |
NAKASHIMA MISAKO 独立行政法人国立長寿医療研究センター, 再生歯科医療研究部, 部長 (20207773)
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| Co-Investigator(Kenkyū-buntansha) |
IOHARA Koichiro 歯科口腔先進医療開発センター, 再生歯科医療研究部, 室長 (60435865)
WATANABE Atsushi 国立長寿医療研究センター, 共同利用推進室, 室長 (90321843)
NAKAMURA Hiroshi 愛知学院大学, 歯学部, 教授 (40064878)
MURAKAMI Masashi 歯科口腔先進医療開発センター, 再生歯科医療研究部, 研究員 (30614531)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2013: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Keywords | 細管象牙質形成 / 加圧刺激 / 歯髄幹細胞 / iPS細胞 / 間葉系幹細胞 / う蝕治療 |
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| Research Abstract |
The present study aimed at the induction of iPS cells into odontoblastic differentiation in response to mechanical compression of the three-dimensional silicon membrane scaffold with dentinal tubule-like pores. The membrane was precoated with optimal method and materials to enhance iPS cell attachment. The optimal conditions, which were 19.6 kPa in compression magnitude, 4.0x10 5 cells/cm2 in cell density, and 9 hours of loading time, efficiently induced odontoblastic differentiation, showing significantly increased expression of odontoblast specific markers and enhanced elongation of cellular processes into the pore of membrane, a typical morphological feature of odontoblasts. In addition, up-regulation of BMP7 and Wnt10a and the involvement of the MAPK signaling pathway were observed. These results suggested the importance of three-dimensional physical structure of the scaffold in odontoblastic differentiation.
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