Developing of differentiation inducing therapy on oral cancer by targeting transcription factor Brachyury which regulates cancer stem cells
Project/Area Number |
23390465
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Surgical dentistry
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Research Institution | Kyushu University |
Principal Investigator |
SUGIURA Tsuyoshi 九州大学, 歯学研究科(研究院), 准教授 (40322292)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
|
Keywords | Brachyury / 癌幹細胞 / 上皮間葉移行 / 腺様嚢胞癌 / 口腔癌 |
Research Abstract |
We reported that the T-box transcription factor Brachyury is a potential regulator of cancer stem cells (CSCs). Specifically, growth of CSCs was controlled by Brachyury knockdown in AdCC. Since CSCs are resistant to chemotherapy and radiotherapy, this finding provides a new principle for therapies targeting CSCs. In the present study, we established that Brachyury knockdown suppresses chemo- and radioresistance in vitro. Brachyury was knocked down by transfecting Brachyury short hairpin RNA (shRNA) into the AdCC CSC cell line, ACCS-M GFP. Brachyury knockdown significantly inhibited cell migration and invasion and suppressed chemoresistance. Furthermore, ACCS-M GFP radioresistance was significantly suppressed by Brachyury knockdown. Brachyury knockdown significantly sensitized ACCS-M GFP cells to chemoradiotherapy. This study demonstrates that Brachyury knockdown reduces invasiveness and chemo- and radioresistance of CSCs in vivo.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] CD82 inhibits canonical Wnt signalling by controlling the cellular distribution of beta-catenin in carcinoma cells2012
Author(s)
Chigita, S., Sugiura, T ., Abe, M., Kobayashi, Y., Shimoda, M., Onoda, M. & Shirasuna, K
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Journal Title
International journal of oncology
Volume: 41
Issue: 6
Pages: 2021-2028
DOI
Related Report
Peer Reviewed
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[Journal Article] T-box transcription factor Brachyury expression is correlated with epithelial-mesenchymal transition and lymph node metastasis in oral squamous cell carcinoma2012
Author(s)
Imajyo, I., Sugiura, T., Kobayashi, Y., Shimoda, M., Ishii, K., Akimoto, N., Yoshihama, N., Kobayashi, I. & Mori, Y
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Journal Title
International journal of oncology
Volume: 41
Issue: 6
Pages: 1985-1995
DOI
Related Report
Peer Reviewed
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[Journal Article] The T-box transcription factor Brachyury regulates epithelial-mesenchymal transition in association with cancer stem-like cells in adenoid cystic carcinoma cells2012
Author(s)
Shimoda, M., Sugiura, T., Imajyo, I., Ishii, K., Chigita, S., Seki, K., Kobayashi, Y. & Shirasuna, K
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Journal Title
BMC cancer
Volume: 12
Issue: 1
Pages: 377-377
DOI
Related Report
Peer Reviewed
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