| Project/Area Number |
23390468
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
IKEBE Tetsuro 福岡歯科大学, 歯学部, 教授 (20202913)
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| Co-Investigator(Kenkyū-buntansha) |
HAYAKAWA Hiroshi 福岡歯科大学, 口腔歯学部, 教授 (70150422)
鍛治屋 浩 (鍛冶屋 浩) 福岡歯科大学, 歯学部, 講師 (80177378)
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| Co-Investigator(Renkei-kenkyūsha) |
KAJIYA Hiroshi 福岡歯科大学, 口腔歯学部, 講師 (80177378)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Keywords | 活性酸素 / 口腔癌 / ケラチノサイト / 細胞老化 / 癌抑制遺伝子 / エピジェネティック修飾 / 老化マーカー / EMT / TRP |
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| Research Abstract |
In order to investigate the mechanism how reactive oxygen species (ROS) induce carcinogenesis of keratinocytes, normal human kerainocytes (NHEK) and oral squamous carcinoma cells (SCCs) were treated with hydrogen peroxide, and compared for the expressions of cellular senescence-associated molecules that is thought to be one of the tumor suppressor mechanisms. Hydrogen peroxide increased the expressions of senescence associated-beta-galactosidase and p16(INK4a) and induced the growth arrest in G0/G1 phase, in NHEK, but not SCC cells. Methylation level in p16 (INK4a) promoter was reduced with hydrogen peroxide in NHEK, but not SCCs. Methylation inhibitor 5-Azac enhanced the expression of p16 (INK4a) in NHEK, but not SCCs. The expression of DNA methyltransferase DNMT1 was suppressed in NHEK, but not SCCs. The inhibition of cellular senescence through the epigenetic modification on p16 (INK4a) expression may play an important role in carcinogenesis of keratinocytes.
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