| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Research Abstract |
Gingival epithelial cells function as innate host defense barriers to prevent intrusion by periodontal bacteria. Nevertheless, Porphyromonas gingivalis can enter these cells and pass through the epithelial barrier into deeper tissues. In this study, P. gingivalis induced cellular invagination to internalize the pathogen. The bacteria were sorted to early endosomes, and the half of them were transported to recycling pathway via SNARE protein VAMP2, which allowed the bacterial exit to the extracellular circumstances, a mechanism of cell-to-cell spread within periodontal tissues. The intracellular pathogens impaired fundamental cellular functions within the cargo. While, remaining half were sorted to lytic compartments, including autophagosomes. The machinery was found to be formed in contact sites between endoplasmic reticulum and mitochondria, which is a very unique finding indicating that 2 different organelle make a concerted effort to the formation of another organelle.
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