Periodontal disease susceptibility evaluated on membrane traffic machinery
| Project/Area Number |
23390477
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
AMANO Atsuo 大阪大学, 歯学研究科(研究院), 教授 (50193024)
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| Co-Investigator(Kenkyū-buntansha) |
KUBONIWA Masae 大阪大学, 歯学部附属病院, 講師 (00303983)
FURUTA Nobumichi 大阪大学, 大学院・歯学研究科, 助教 (50452446)
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| Project Period (FY) |
2011-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 歯学 / 歯周病菌 / 歯周病 / 細菌・組織 / メンブレントラフィック / 細胞内侵入 / オートファジー / 細菌感染 / 細胞・組織 / SNARE |
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| Research Abstract |
Gingival epithelial cells function as innate host defense barriers to prevent intrusion by periodontal bacteria. Nevertheless, Porphyromonas gingivalis can enter these cells and pass through the epithelial barrier into deeper tissues. In this study, P. gingivalis induced cellular invagination to internalize the pathogen. The bacteria were sorted to early endosomes, and the half of them were transported to recycling pathway via SNARE protein VAMP2, which allowed the bacterial exit to the extracellular circumstances, a mechanism of cell-to-cell spread within periodontal tissues. The intracellular pathogens impaired fundamental cellular functions within the cargo. While, remaining half were sorted to lytic compartments, including autophagosomes. The machinery was found to be formed in contact sites between endoplasmic reticulum and mitochondria, which is a very unique finding indicating that 2 different organelle make a concerted effort to the formation of another organelle.
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Report
(4 results)
Research Products
(118 results)
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[Journal Article] Autophagosome forms at the ER-mitochondria contact sites2013
Author(s)
Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Ohmori H, Noda T, Haraguchi T, Hiraoka Y, Amano A, Yoshimori T
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Journal Title
Nature
Volume: 495(7441)
Pages: 389-393
Related Report
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[Journal Article] Autophagosomes form at ER–mitochondria contact sites2013
Author(s)
Hamasaki M, Furuta N, Matsuda A, Nezu A, Yamamoto A, Fujita N, Oomori H, Noda T, Haraguchi T, Hiraoka Y, Amano A, Yoshimori T
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Journal Title
Nature
Volume: 495
Pages: 389-393
DOI
Related Report
Peer Reviewed
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[Journal Article] Involvement of a periodontal pathogen, Porphyromonas gingivalis on the pathogenesis of nonalcoholic2012
Author(s)
Yoneda M, Naka S, Nakano K, Wada K, Endo H, Mawatari H, Imajo K, Nomura R, Hokamura K, Ono M, Murata S, Tohnai I, Sumida Y, Shima T, Kuboniwa M, Umemura K, Kamisaki Y, Amano A, Okanoue T, Ooshima T, Nakajima A.
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Journal Title
BMC gastroenterology
Volume: 12
Pages: 12-16
DOI
Related Report
Peer Reviewed
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[Journal Article] Distribution and molecular characterization of Porphyromonas gulae carrying a new fimA genotype2012
Author(s)
Yamasaki Y, Nomura R, Nakano K, Inaba H, Kuboniwa M, Hirai N, Shirai M, Kato Y, Murakami M, Naka S, Iwai S, Matsumoto-Nakano M, Ooshima T, Amano A, Asai F
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Journal Title
Veter inar y Microbiology
Volume: 161(1)
Pages: 196-205
DOI
Related Report
Peer Reviewed
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[Journal Article] The collagen-binding protein of Streptococcus mutans is involved in haemorrhagic stroke2011
Author(s)
Nakano K, Hokamura K, Taniguchi N, Wada K, Kudo C, Nomura R, Kojima A, Naka S, Muranaka Y, Thura M, Nakajima A, Masuda K, Nakagawa I, Speziale P, Shimada N, Amano A, Kamisaki Y, Tanaka T, Umemura K, Ooshima T.
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Journal Title
Nat Commun
Volume: 2
Pages: 485-485
DOI
Related Report
Peer Reviewed
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[Journal Article] Characterization of aortic aneurysms in cardio- vascular disease patients harboring Porphyromonas gingivalis2011
Author(s)
Nakano K, Wada K, Nomura R, Nemoto H, Inaba H, Kojima A, Naka S, Hokamura K, Mukai T, Nakajima A, Umemura K, Kamisaki Y, Yoshioka H, Taniguchi K, Amano A, Ooshima T
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Journal Title
Oral disease
Volume: 17(4)
Pages: 370-378
DOI
Related Report
Peer Reviewed
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[Presentation] Tumor necrosis factor  in saliva may be a novel marker of carotid atherosclerosis: The Suita Study2014
Author(s)
Kosaka T, Kokubo Y, Ono T, Sekine S, Kida M, Kikui M, Yamamoto M, Watanabe M, Amano A, Maeda Y, Miyamoto Y
Organizer
European Stroke Conference
Place of Presentation
Nice
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