Epidemiological study on hemo-protozoan diseases in Vietnamese livestock animals

• Project/Area Number: 23405041
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: YOKOYAMA Naoaki 帯広畜産大学, 原虫病研究センター, 教授 (80301802)
• Co-Investigator(Kenkyū-buntansha): INOUE Noboru 帯広畜産大学, 原虫病研究センター, 教授 (10271751)
• Project Period (FY): 2011-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: ベトナム / 家畜 / 原虫病 / バベシア / タイレリア / 住血性原虫病 / 疫学 / トリパノソーマ / 疫学調査 / 遺伝子診断法 / 遺伝子多型解析法 / ウシ / 水牛 / 遺伝子診断 / 遺伝子多型

Outline of Final Research Achievements

In this study, we analyzed the blood DNA samples sourced from livestock animals (cattle, buffaloes, goats, and sheep) bred in Hanoi and Hue provinces of Vietnam for Babesia, Theileria, and Trypanosoma infections using genetic and serological diagnostic assays that we established in our laboratory. The results demonstrated that the Babesia (Babesia bovis, Babesia bigemina), Theileria (Theileria orientalis), and Trypanosoma (Trypanosoma thileri) infections are common among livestock animals in Vietnam. The findings of the present study might be useful to investigate the disease outbreaks in the endemic areas as well as to design improved preventive strategies against hemo-protozoan diseases (babesiosis, theileriosis, and trypanosomiasis) in Vietnam.

Research Products

• [Journal Article] Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes. (2015)
  • Author(s): Yokoyama N, Sivakumar T, Tuvshintulga B, Hayashida K, Igarashi I, Inoue N, Long PT, Lan DT.
  • Journal Title: Infect. Genet. Evol. Volume: 30 Pages: 288-295
  • DOI: 10.1016/j.meegid.2014.12.035
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evolution and genetic diversity of Theileria. (2014)
  • Author(s): Sivakumar T, Hayashida K, Sugimoto C, Yokoyama N.
  • Journal Title: Infect. Genet. Evol. Volume: 27 Pages: 250-263
  • DOI: 10.1016/j.meegid.2014.07.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular and seroepidemiological survey of Babesia bovis and Babesia bigemina infections in cattle and water buffaloes in the central region of Vietnam. (2014)
  • Author(s): Li Y, Luo Y, Cao S, Terkawi MA, Lan DT, Long PT, Yu L, Zhou M, Gong H, Zhang H, Zhou J, Yokoyama N, Suzuki H, Xuan X.
  • Journal Title: Trop. Biomed. Volume: 31 Pages: 406-413
  • Peer Reviewed
• [Journal Article] Babesia ovata in cattle: A review. (2013)
  • Author(s): Sivakumar, T., and Yokoyama, N.
  • Journal Title: Jpn. J. Vet. Parasitol. Volume: 12 Pages: 11-20
  • Peer Reviewed
• [Journal Article] A PCR Based Survey of <i>Babesia ovata</i> in Cattle from Various Asian, African and South American Countries (2013)
  • Author(s): Yoshinari, T., Sivakumar, T., Asada, M., Battsetseg, B., Huang, X., Lan, D. T. B., Inpankaew, T., Ybanez, A. P., Alhassan, A., Thekisoe, O. M. M., de macido, A. C., Inokuma, H., Igarashi, I., and Yokoyama, N
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 2 Pages: 211-214
  • DOI: 10.1292/jvms.12-0329
  • NAID: 130001879670
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] PCR Detection and Genetic Diversity of Bovine Hemoprotozoan Parasites in Vietnam (2013)
  • Author(s): Sivakumar, T., Lan, D.T., Long, P.T., Yoshinari, T., Tattiyapong, M., Guswanto, A., Okubo, K., Igarashi, I., Inoue, N., Xuan, X., and Yokoyama, N
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 11 Pages: 1455-1462
  • DOI: 10.1292/jvms.13-0221
  • NAID: 130003362251
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] A Clinical Case of Severe Anemia in a Sheep Coinfected with Mycoplasma ovis and Candidatus Mycoplasma haemovis' in Hokkaido, Japan (2012)
  • Author(s): Tagawa, M., Takeuchi, T., Fujisawa, T., Yamamoto, S., Konno, Y., Matsumoto, K., Yokoyama, N., and Inokuma, H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 74 Issue: 1 Pages: 99-102
  • DOI: 10.1292/jvms.11-0296
  • NAID: 130001032844
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Phylogenetic relationships of Mongolian Babesia bovis isolates based on the merozoite surface antigen (MSA)-1, MSA-2b, and MSA-2c genes (2012)
  • Author(s): Altangerel, K., Sivakumar, T., Battsetseg, B., Battur, B., Ueno, A., Igarashi, I., and Yokoyama, N
  • Journal Title: Vet. Parasitol Volume: 184 Issue: 2-4 Pages: 309-316
  • DOI: 10.1016/j.vetpar.2011.09.021
  • Peer Reviewed
• [Journal Article] Molecular and serological prevalence of Babesia bovis and Babesia bigemina in cattle from central region of Syria (2012)
  • Author(s): Terkawi, M., A., Alhasan, H., Huyen, N. X., Sabagh, A., Awier. K., Cao, S., Goo, Y.-K., Aboge, G., Yokoyama, N., Nishikawa, Y., Kalb-Allouz, A. K., Tabbaa, D., Igarashi, I., and Xuan, X
  • Journal Title: Vet. Parasitol Volume: 187 Issue: 1-2 Pages: 307-11
  • DOI: 10.1016/j.vetpar.2011.12.038
  • Peer Reviewed
• [Journal Article] Genetic detection of Babesia bigemina from Mongolian cattle using apical membrane antigen-1 gene-based PCR assay. (2012)
  • Author(s): Sivakumar T, Altangerel K, Battsetseg B, Battur B, Aboulaila M, et al.
  • Journal Title: Veterinary Parasitology Volume: 187 Issue: 1-2 Pages: 17-22
  • DOI: 10.1016/j.vetpar.2012.01.008
  • Peer Reviewed
• [Journal Article] PCR Detection of Babesia ovata from Cattle Reared in Japan and Clinical Significance of Co-Infection with Theileria orientalis (2012)
  • Author(s): Sivakumar, T., Tagawa, M., Yoshinari, T., Ybanez, A. P., Igarashi, I., Ikehara, Y., Hata, H., Kondo, S., Matsumoto, K., Inokuma, H., and Yokoyama, N
  • Journal Title: J. Clin. Microbiol Volume: 50 Issue: 6 Pages: 2111-3
  • DOI: 10.1128/jcm.00220-12
  • Peer Reviewed
• [Journal Article] C-terminal region of 48-kDa rhoptry protein for serological detection of Babesia caballi antibodies in horses (2012)
  • Author(s): Terkawi MA, Alhasan H, Ueno A, Ratthanophart J, Luo Y, Cao S, Kamvingkird K, Aboulaila M, et al.
  • Journal Title: Parasitology International Volume: 61 Issue: 3 Pages: 493-496
  • DOI: 10.1016/j.parint.2012.02.006
  • Peer Reviewed
• [Journal Article] Genetic diversity of Theileria orientalis in tick vectors detected in Hokkaido and Okinawa, Japan. (2012)
  • Author(s): Yokoyama, N., Sivakumar, T., Ota, N., Igarashi, I., Nakamura, Y., Yamashina, H., Matsui, S., Fukumoto, N., Hata, H., Kondo, S., Oshiro, M., Zakimi, S., Kuroda, Y., Kojima, N., Matsumoto, K., and Inokuma, H.
  • Journal Title: Infect. Genet. Evol. Volume: 12 Issue: 8 Pages: 1669-1675
  • DOI: 10.1016/j.meegid.2012.07.007
  • Peer Reviewed
• [Journal Article] Dual Presence of <i>Anaplasma phagocytophilum</i> and Its Closely Related <i>Anaplasma</i> sp. in Ixodid Ticks in Hokkaido, Japan, and Their Specific Molecular Detection (2012)
  • Author(s): Ybanez, A. P., Matsumoto, K., Kishimoto, T., Yokoyama, N., and Inokuma, H.
  • Journal Title: Journal of Veterinary Medical Science Volume: 74 Issue: 12 Pages: 1551-1560
  • DOI: 10.1292/jvms.12-0197
  • NAID: 130001879718
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Prevalence and Risk Factor Analysis of Bovine Hemoplasma Infection by Direct PCR in Eastern Hokkaido, Japan (2012)
  • Author(s): Tagawa M, Ybafiez AP, Matsumoto K, Yokoyama N, Inokuma H
  • Journal Title: Journal of Veterinary Medical Science Volume: 74 Issue: 9 Pages: 1171-1176
  • DOI: 10.1292/jvms.12-0118
  • NAID: 130001879758
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] A PCR-based survey of selected Babesia and Theileria parasites in cattle in Sri Lanka (2012)
  • Author(s): Sivakumar, T., Kothalawala, H., Abeyratne, S. A., Vimalakumar, S. C., Meewewa, A. S., Hadirampela, D. T., Puvirajan, T., Sukumar, S., Kuleswarakumar, K., Chandrasiri, A. D., Igarashi, I., and Yokoyama N
  • Journal Title: Vet. Parasitol Volume: 190 Issue: 1-2 Pages: 263-7
  • DOI: 10.1016/j.vetpar.2012.05.014
  • Peer Reviewed
• [Journal Article] Molecular detection and identification of Babesia bovis and Babesia bigemina in cattle in northern Thailand (2012)
  • Author(s): Cao, et al.
  • Journal Title: Parasitology Research Volume: 111 Issue: 3 Pages: 1259-1266
  • DOI: 10.1007/s00436-012-2960-4
  • Peer Reviewed
• [Journal Article] Genotypic diversity of Theileria orientalis detected from cattle grazing in Kumamoto and Okinawa prefectures of Japan. (2011)
  • Author(s): Yokoyama, N.
  • Journal Title: J. Vet. Med. Sci. Volume: 73 Pages: 305-312
  • NAID: 120006390575
  • Peer Reviewed
• [Journal Article] Spherical body protein 4 is a new serological antigen for the global detection of Babesia bovis infection in cattle (2011)
  • Author(s): Terkawi, M. A., Huyen, N. X., Wibowo, P. E., Seuseu, F. J., Aboulaila, M., Ueno, A., Goo, Y.-K., Yokoyama, N., Xuan, X., and Igarashi, I
  • Journal Title: Clin Vaccine. Immunol Volume: 18 Issue: 2 Pages: 337-342
  • DOI: 10.1128/cvi.00388-10
  • Peer Reviewed
• [Journal Article] Rapid discrimination and quantification of Theileria orientalis types using ribosomal DNA internal transcribed spacers. (2011)
  • Author(s): Kamau, J.
  • Journal Title: Infect. Genet. Evol. Volume: 11 Issue: 2 Pages: 407-414
  • DOI: 10.1016/j.meegid.2010.11.016
  • Peer Reviewed
• [Journal Article] Molecular and serological prevalence of Babesia bovis and Babesia bigemina in water buffaloes in the northeast region of Thailand (2011)
  • Author(s): Terkawi, M. A., Huyen, N. X., Shinuo, C., Inpankaew, T., Maklon, K., Aboulaila, M., Ueno, A., Goo, Y. K., Yokoyama, N., Jittapalapong, S., Xuan, X., and Igarashi, I
  • Journal Title: Vet. Parasitol Volume: 178 Issue: 3-4 Pages: 201-207
  • DOI: 10.1016/j.vetpar.2011.01.041
  • Peer Reviewed
• [Journal Article] Molecular epidemiological survey of Theileria orientalis in Thua Thien Hue province, Vietnam. (2011)
  • Author(s): Khukhuu, A.
  • Journal Title: J. Vet. Med. Sci. Volume: 73 Pages: 701-705
  • Peer Reviewed
• [Journal Article] Phylogenetic analysis of Theileria orientalis in cattle bred in Fujian province, China. (2011)
  • Author(s): Sivakumar, T.
  • Journal Title: J. Protozool. Res. Volume: 21 Pages: 14-19
  • Peer Reviewed
• [Journal Article] The first survey of Theileria orientalis infection in Mongolian cattle. (2011)
  • Author(s): Altangerel, K.
  • Journal Title: Vet. Parasitol. Volume: 182 Issue: 2-4 Pages: 343-348
  • DOI: 10.1016/j.vetpar.2011.05.040
  • Peer Reviewed
• [Journal Article] Molecular prevalence of different genotypes of Theileria orientalis detected from cattle and water buffaloes in Thailand. (2011)
  • Author(s): Altangerel, K.
  • Journal Title: J. Parasitol. Volume: 97 Issue: 6 Pages: 1075-1079
  • DOI: 10.1645/ge-2846.1
  • Peer Reviewed
• [Journal Article] Serological survey of Babesia bovis and Babesia bigemina in cattle in South Africa (2011)
  • Author(s): Terkawi, M. A., Thekisoe, O. M. M., Katsande, C., Latif, A. A., Mansb, B. J., Matthee, O., Mkize, N., Mabogoane, N., Marais, F., Yokoyama, N., Xuan, X., and Igarashi, I
  • Journal Title: Vet. Parasitol Volume: 182 Issue: 2-4 Pages: 337-342
  • DOI: 10.1016/j.vetpar.2011.05.047
  • Peer Reviewed
• [Journal Article] In vitro growth inhibitory effect of (-)-Epigallocatechin-3-gallate from green tea on the growth of equine Babesia parasites. (2011)
  • Author(s): AbouLaila, M.
  • Journal Title: J. Protozool. Res. Volume: 21 Pages: 30-35
  • Peer Reviewed
• [Presentation] Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes. (2015)
  • Author(s): 横山直明、Sivakumar Thillaiampalam、五十嵐郁男、井上昇
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 杏林大学（東京）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Genetic diversity of Babesia bovis merozoite surface antigen genes: Implications for strain verification and immune control. (2015)
  • Author(s): Sivakumar Thillaiampalam、横山直明
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 杏林大学（東京）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Genetic diversity of Theileria. (2014)
  • Author(s): 横山直明、Sivakumar Thillaiamapalam、林田京子、杉本千尋
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（札幌）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Babesia ovata: Epidemiology and clinical significance. (2014)
  • Author(s): 横山直明、Sivakumar Thillaiamapalam、五十嵐郁男、猪熊壽
  • Organizer: 第83回日本寄生虫学会大会
  • Place of Presentation: 愛媛大学（愛媛）
  • Year and Date: 2014-03-27 – 2014-03-28
• [Presentation] Molecular epidemiology and clinical significance of Theileria orientalis and Babesia ovata in cattle. (2013)
  • Author(s): T. Sivakumar、横山直明、五十嵐郁男
  • Organizer: 第155回日本獣医学会
  • Place of Presentation: 東京大学
  • Invited
• [Presentation] MSA-1 gene-based genetic diversity of Babesia bovis detected in cattle populations in Thailand, Brazil, and Ghana. (2013)
  • Author(s): 横山直明、長野大輔、T. Sivakumar、五十嵐郁男
  • Organizer: 第155回日本獣医学会
  • Place of Presentation: 東京大学
• [Presentation] Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia. (2013)
  • Author(s): 五十嵐郁男、T. Munkhjargal、T. Sivakumar、横山直明
  • Organizer: 第155回日本獣医学会
  • Place of Presentation: 東京大学
• [Presentation] Molecular survey of bovine vector-borne oathogens in Cebu, Philippines. (2013)
  • Author(s): A. Ybanez、T. Sivakumar、井上 昇、松本高太郎、猪熊 壽、横山直明
  • Organizer: 第155回日本獣医学会
  • Place of Presentation: 東京大学
• [Presentation] 放牧牛に見られた小型ピロプラズマとヘモプラズマの干渉現象 (2013)
  • Author(s): 田川道人、松本高太郎、横山直明、猪熊 壽
  • Organizer: 第155回日本獣医学会
  • Place of Presentation: 東京大学
• [Presentation] Epidemiology of bovine Babesia and Theileria parasites in Sri Lanka (2012)
  • Author(s): T. Sivakumar、五十嵐郁男、横山直明
  • Organizer: 第154回日本獣医学会
  • Place of Presentation: 岩手大学
• [Presentation] Genetic diversity of Theileria orientalis in tick vectors detected in Hokkaido, Japan. (2012)
  • Author(s): 横山直明、T. Sivakumar、五十嵐郁男、松本高太郎、猪熊 壽
  • Organizer: 第154回日本獣医学会
  • Place of Presentation: 岩手大学
• [Presentation] Development and evaluation of Babesia bigemina-specific nested PCR method based on the gene encoding apical membrane antigen-1 (AMA-1) (2011)
  • Author(s): Thillaiampalam Sivakumar
  • Organizer: 日本獣医学会
  • Place of Presentation: 大阪府立大学
• [Presentation] The first survey of Theileria orientalis infection in Mongolian cattle (2011)
  • Author(s): 横山 直明
  • Organizer: 日本獣医学会
  • Place of Presentation: 大阪府立大学