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Study on genetic diversity of highly endemic pathogenic intestinal porotozans in Indonesia

Research Project

Project/Area Number 23406006
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Parasitology (including Sanitary zoology)
Research InstitutionKanazawa University

Principal Investigator

MASAHARU Tokoro  金沢大学, 医学系, 講師 (30338024)

Co-Investigator(Kenkyū-buntansha) YOSHIKAWA Hisao  奈良女子大学, 自然科学系, 准教授 (50191557)
Project Period (FY) 2011-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2013: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords分子分類 / 腸管寄生原虫 / インドネシア / 種内多型 / 遺伝子型 / 分子疫学 / 人獣共通感染症 / 国際研究者交流 / 遺伝子型解析 / GIS / 分泌型抗体 / 尿中抗体 / ブラストシスチス / ジアルジア / アメーバ / トリコモナス
Outline of Final Research Achievements

The molecular taxonomic method was applied in investigations of various protozoan infections in Indonesia, and the endemicity data was epidemiologically assessed from the view of mutual relations with the characteristics, such as age, gender, of target population.
The results showed following findings: (1) All the investigated protozoan groups, Amoebazoa, Fornicata, Parabasalia, and Stramenopiles, were highly endemic in the target site. (2) Age-related and gender-related differences of protozoan prevalences were indicated in targeted student population. (3) Giardial prevalence reduction on aging was attributed to assemblage A prevalence, which was reduced in higher age layers. (4) The endemicity of Pentatrichomonas hominis was maintained by zoonotic transmissions of a specific strain in the area.
Molecular epidemiological methods targeting various protozoan species could improve our understanding of the endemicity of those infections.

Report

(4 results)
  • 2014 Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (30 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (26 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Genetic Characterization of Clinical Acanthamoeba Isolates Using Gene Loci of Nuclear and Mitochondrial Small Subunit Ribosomal RNA2013

    • Author(s)
      Md Moshiur RAHMAN, Kengi YAGITA, Akira KOBAYASHI, Yosaburo OIKAWA, Amjad I.A. HUSSEIN, Takahiro MATSUMURA, Masaharu TOKORO
    • Journal Title

      Korean Journal of Parasitology

      Volume: 51(4) Issue: 4 Pages: 401-411

    • DOI

      10.3347/kjp.2013.51.4.401

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Giardiasis in a patient undergoing chemotherapy for retinoblastoma and acute myelogenous leukemia.2013

    • Author(s)
      Otsubo K, Sakaki-Nakatsubo H, Taneichi H, Nomura K, Miyawaki T, Tokoro M, Kanegane H
    • Journal Title

      Pediatlics International

      Volume: 55(4) Issue: 4 Pages: 524-527

    • DOI

      10.1111/ped.12078

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivo laser confocal microscopy findings of radial keratoneuritis in patients with early stage acanthamoeba keratitis.2013

    • Author(s)
      Kobayashi A, Yokogawa H, Yamazaki N, Ishibashi Y, Oikawa Y, Tokoro M, Sugiyama K
    • Journal Title

      Ophthalmology

      Volume: 120(7) Issue: 7 Pages: 1348-1353

    • DOI

      10.1016/j.ophtha.2012.12.038

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Presentation] ベーシックレクチャー招待講演「寄生虫感染症」2014

    • Author(s)
      所 正治
    • Organizer
      第25回日本臨床微生物学会
    • Place of Presentation
      名古屋国際会議場,名古屋
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] A comprehensive molecular analysis for various intestinal protozoan parasites2013

    • Author(s)
      Tokoro, Masaharu; Yoshikawa, Hisao; Syafruddin, Din
    • Organizer
      Annual Molecular Parasitology Meeting 2013 (24th)
    • Place of Presentation
      Marine Biology Laboratory, Woods Hole, MA, United States
    • Related Report
      2013 Annual Research Report
  • [Presentation] 途上国におけるEntamoeba属の感染実態2013

    • Author(s)
      荒山駿介、Din Syafruddin、所正治
    • Organizer
      第31回北陸病害動物研究会
    • Place of Presentation
      金沢医科大学、金沢
    • Related Report
      2013 Annual Research Report
  • [Presentation] 腸管寄生原虫:網羅的検出により見えてくるもの2012

    • Author(s)
      所正治
    • Organizer
      第81回日本寄生虫学会大会
    • Place of Presentation
      兵庫医科大(兵庫県)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] ブラストシスチスの感染伝播に関する研究:2009年に実施したインドネシア・スンバ島での調査2012

    • Author(s)
      吉川尚男
    • Organizer
      第81回日本寄生虫学会大会
    • Place of Presentation
      兵庫医科大(兵庫県)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Giardia intestinalis臨床分離株による薬剤感受性評価実験2012

    • Author(s)
      松村隆弘
    • Organizer
      第81回日本寄生虫学会大会
    • Place of Presentation
      兵庫医科大(兵庫県)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Comprehensive molecular detection methods revealed a true image of protozoan endemicity in Southwest Sumba district, Indonesia2012

    • Author(s)
      所正治
    • Organizer
      第81回日本寄生虫学会大会
    • Place of Presentation
      兵庫医科大(兵庫県)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Trichotnonads Species Distributing in Southwest Sumba District, Indonesia2012

    • Author(s)
      Ali B Kusuma
    • Organizer
      International conferences on Biomedical Sciences 2012
    • Place of Presentation
      バンドン工科大(インドネシア)
    • Year and Date
      2012-02-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Opportunistic Intestinal Parasitic Infection in HIV Positive Patients in Bali Island, Indonesia2012

    • Author(s)
      Mudyawati Kamaruddin, Ei Kawahara, Din Syafruddin, Masaharu Tokoro
    • Organizer
      The 6th Nagasaki Symposium on Tropical and Emerging Infectious Diseases
    • Place of Presentation
      Ryojun Auditorium, Nagasaki Univ., Nagasaki, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] First report of T5 genotypes of Acanthamoeba spp. from clinical isolates of human keratitis cases in Japan2012

    • Author(s)
      Md Moshiur Rahman, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A. Hussein, Takahiro Matsumura, Masaharu Tokoro
    • Organizer
      The 6th Nagasaki Symposium on Tropical and Emerging Infectious Diseases
    • Place of Presentation
      Ryojun Auditorium, Nagasaki Univ., Nagasaki, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] ブラストシスチスのサブタイプ同定に用いられているPCRプライマーの特異性について2012

    • Author(s)
      山中美紀、所正治、ディン・シャフルディン、吉川尚
    • Organizer
      第68回日本寄生虫学会西日本支部大会
    • Place of Presentation
      奈良県文化会館(奈良)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Improvement of molecular detection methods for intestinal protozoan parasites2012

    • Author(s)
      Shunsuke Arayama,Masaya Nakazawa, Hisao Yoshikawa, Masaharu Tokoro,Din Syafruddin
    • Organizer
      12th Asian-Pacific Congress for Parasitic Zoonoses
    • Place of Presentation
      Takikawa Memorial Hall, Kobe Univ., Kobe,Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] Genetic diversity of clinical isolated Acanthamoeba spp. from keratitis cases in Japan2012

    • Author(s)
      Md. Moshiur Rahman, Akira Kobayashi, Yosaburo Oikawa, Amjad I.A.Hussein, Takahiro Matsumura, Masaharu Tokoro
    • Organizer
      12th Asian-Pacific Congress for Parasitic Zoonoses
    • Place of Presentation
      Takikawa Memorial Hall, Kobe Univ., Kobe,Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] Molecular phylogeny of Blastocystis isolates from rodents in the Sumba Island, Indonesia2012

    • Author(s)
      Maai Katsumata, Hisao Yoshikawa, Masaharu Tokoro,Din Syafruddin
    • Organizer
      12th Asian-Pacific Congress for Parasitic Zoonoses
    • Place of Presentation
      Takikawa Memorial Hall, Kobe Univ., Kobe,Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] A comprehensive genetic identification approach revealed endemic status of intestinal protozoan parasites in Indonesia2012

    • Author(s)
      Masaharu Tokoro, Hisao Yoshikawa, Din Syafruddin
    • Organizer
      12th Asian-Pacific Congress for Parasitic Zoonoses
    • Place of Presentation
      Takikawa Memorial Hall, Kobe Univ., Kobe,Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] Entamoeba属の網羅的検出同定法の問題点2012

    • Author(s)
      荒山駿介、松村隆弘、Rahman Md Mshiur、所 正治
    • Organizer
      第30回北陸病害動物研究会
    • Place of Presentation
      三国文化未来館(福井)
    • Related Report
      2012 Annual Research Report
  • [Presentation] A comprehensive genetic identification approach revealed endemic status of intestinal protozoan parasites in Indonesia2012

    • Author(s)
      M. Tokoro, H. Yoshikawa, D. Syafruddin
    • Organizer
      15th International Congress on Infectious Diseases
    • Place of Presentation
      Centara Grand & Bangkok Convention Centre,Bangkok, Thailand
    • Related Report
      2012 Annual Research Report
  • [Presentation] Molecular Epidemiology of Intestinal Trichomonads on the Island of Sumba, Indonesia2011

    • Author(s)
      Anggi PN Hidayati
    • Organizer
      The 5^<th> International Eijkman Conference
    • Place of Presentation
      Hotel Borobudur(インドネシア)
    • Year and Date
      2011-11-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Entamoeba hartmanniの18S rRNA遺伝子領域の多型解析2011

    • Author(s)
      松村隆弘
    • Organizer
      第67回日本寄生虫学会西日本支部大会
    • Place of Presentation
      いしかわアートシアター(石川県)
    • Year and Date
      2011-10-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 蛍光抗体試薬のブラストシスチス検出感度について2011

    • Author(s)
      勝間田真愛
    • Organizer
      第67回日本寄生虫学会西日本支部大会
    • Place of Presentation
      いしかわアートシアター(石川県)
    • Year and Date
      2011-10-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Inter/intra-species genetic diversities in amebic protozoans ; Entamoeba spp.and Acanthamoeba spp2011

    • Author(s)
      所正治
    • Organizer
      第17回日独原虫病シンポジウム
    • Place of Presentation
      奈良女子大(奈良県)(招待講演)
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular epidemiology of intestinal trichomonads on the island of Sumba, Indonesia2011

    • Author(s)
      Anggie P.N.Hidayati
    • Organizer
      第17回日独原虫病シンポジウム
    • Place of Presentation
      奈良女子大(奈良県)
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Comprehensive identification of protozoan parasites : distribution of species and genotypes in Sumba Island, Indonesia2011

    • Author(s)
      所正治
    • Organizer
      第80回日本寄生虫学会
    • Place of Presentation
      慈恵会医科大(東京都)
    • Year and Date
      2011-07-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 途上国におけるGiardia intestinalisの感染実態2011

    • Author(s)
      天野香織
    • Organizer
      第29回北陸病害動物研究会
    • Place of Presentation
      金沢大学(石川県)
    • Year and Date
      2011-07-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] 非病原性アメーバを含むEntamoeba属の網羅的検出2011

    • Author(s)
      石井裕子
    • Organizer
      第29回北陸病害動物研究会
    • Place of Presentation
      金沢大学(石川県)
    • Year and Date
      2011-07-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] PCR法のピットホール:赤痢アメーバの誤診例2011

    • Author(s)
      松村隆弘
    • Organizer
      第29回北陸病害動物研究会
    • Place of Presentation
      金沢大学(石川県)
    • Year and Date
      2011-07-02
    • Related Report
      2011 Annual Research Report
  • [Remarks] 金沢大学医薬保健研究域医学系寄生虫感染症学ホームページ

    • URL

      http://www.parasitology.jp/research.html

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-04-06   Modified: 2019-07-29  

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