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Screening of novel malaria vaccine candidates with protective immune sera

Research Project

Project/Area Number 23406007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Parasitology (including Sanitary zoology)
Research InstitutionEhime University

Principal Investigator

TSUBOI Takafumi  愛媛大学, プロテオサイエンスセンター, 教授 (00188616)

Co-Investigator(Kenkyū-buntansha) TAKEO Satoru  杏林大学, 医学部, 准教授 (40302666)
TAKASHIMA Eizo  愛媛大, プロテオサイエンスセンター, 准教授 (50366762)
Project Period (FY) 2011-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Keywords原虫 / マラリア / ワクチン / プロテインアレイ / パプアニューギニア / 国際研究者交流 / 感染症 / バイオテクノロジー / ゲノム
Research Abstract

To identify novel malaria vaccine candidates, we first obtained serum samples from the children enrolled in the malaria cohort study in Papua New Guinean. We then established a micro-scale immunoscreening system for high-throughput detection of antigen-antibody reaction. Genome-widely expressed 1,900 parasite proteins by the wheat germ cell-free system covering one-third of the parasite genome were screened by using the immunoscreening system. As a result of the preliminary screening, we identified 650 immunoreactive proteins with the malaria immune serum samples which we previously had enough amount. The result suggests that this screening system would be effective to discover novel malaria vaccine candidates by using Papua New Guinean serum samples.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • Research Products

    (40 results)

All 2013 2012 2011 Other

All Journal Article (25 results) (of which Peer Reviewed: 25 results) Presentation (11 results) (of which Invited: 8 results) Remarks (4 results)

  • [Journal Article] A small molecule glycosaminoglycan mimetic blocks Plasmodium invasion of the mosquito midgut.2013

    • Author(s)
      Mathias DK, Pastrana-Mena R, Ranucci E, Ferruti P, de Beaumont C, Ortega C, Staples GO, Zaia J, Takashima E, Tsuboi T, Verotta L, Dinglasan RR
    • Journal Title

      PLoS Pathogens

      Volume: 9 Issue: 11 Pages: e1003757-e1003757

    • DOI

      10.1371/journal.ppat.1003757

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional comparison of Plasmodium falciparum transmission-blocking vaccine candidates by the standard membrane-feeding assay2013

    • Author(s)
      Miura K, Takashima E, Tsuboi T. (他9名)
    • Journal Title

      Infect Immun

      Volume: 81(12) Issue: 12 Pages: 4377-82

    • DOI

      10.1128/iai.01056-13

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] RALP1 is a rhoptry neck erythrocyte-binding protein of Plasmodium falciparum merozoites and a potential blood-stage vaccine candidate antigen2013

    • Author(s)
      Ito D, Hasegawa T, Miura K, Yamasaki T, Arumugam TU, Thongkukiatkul A, Takeo S, Takashima E, Sattabongkot J, Han ET, Long CA, Torii M, *Tsuboi
    • Journal Title

      Infect Immun

      Volume: 81 Issue: 11 Pages: 4290-4298

    • DOI

      10.1128/iai.00690-13

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and prioritization of merozoite antigens as targets of protective human immunity to Plasmodium falciparum malaria for vaccine and biomarker development.2013

    • Author(s)
      Richards JS, Arumugam TU, Reiling L, Healer J, Hodder AN, Fowkes FJI, Cross N, Langer C, Takeo S, Uboldi AD, Thompson JK, Gilson PR, Coppel RL, Siba PM, King CL, Torii M, Chitnis CE, Narum DL, Mueller I, Crabb BS, Cowman AF, Tsuboi T, Beeson JG.
    • Journal Title

      Journal of Immunology

      Volume: 191 Issue: 2 Pages: 795-809

    • DOI

      10.4049/jimmunol.1300778

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Plasmodium vivax merozoite surface protein 1 paralog is a novel erythrocyte-binding ligand of P. vivax.2013

    • Author(s)
      Cheng Y, Wang Y, Ito D, Kong DH, Ha KS, Chen JH, Lu F, Li J, Wang B, Takashima E, Sattabongkot J, Tsuboi T, Han ET.
    • Journal Title

      Infection and Immunity

      Volume: 81 Issue: 5 Pages: 1585-1595

    • DOI

      10.1128/iai.01117-12

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A member of the CPW-WPC protein family is expressed in and localized to the surface of developing ookinetes2013

    • Author(s)
      Niwat Kangwanrangsan
    • Journal Title

      Malaria Journal

      Volume: 12 Issue: 1 Pages: 129-129

    • DOI

      10.1186/1475-2875-12-129

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phenotypic dissection of a Plasmodium-refractory strain of malaria vector Anopheles stephensi: the reduced susceptibility to P. berghei and P. yoelii2013

    • Author(s)
      Shinzawa N
    • Journal Title

      PLoS One

      Volume: 8

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New insights into acquisition, boosting and longevity of immunity to malaria in pregnant women2012

    • Author(s)
      Fowkes FJ, Tsuboi T. (他12名)
    • Journal Title

      J Infect Dis

      Volume: 206 Issue: 10 Pages: 1612-21

    • DOI

      10.1093/infdis/jis566

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antibodies against a Plasmodium falciparum antigen PfMSPDBL1 inhibit merozoite invasion into human erythrocytes2012

    • Author(s)
      Sakamoto H, Takeo S, Maier AG, Sattabongkot J, Cowman AF, Tsuboi T
    • Journal Title

      Vaccine

      Volume: 30 Issue: 11 Pages: 1972-1980

    • DOI

      10.1016/j.vaccine.2012.01.010

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasmodium vivax gametocyte protein Pvs230 is a novel transmission-blocking vaccine candidate2012

    • Author(s)
      Tachibana, et al.
    • Journal Title

      Vaccine

      Volume: 30 Issue: 10 Pages: 1807-1812

    • DOI

      10.1016/j.vaccine.2012.01.003

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] PfSET10, a Plasmodium falciparum methyltransferase, maintains the active var gene in a poised state during parasite division2012

    • Author(s)
      Volz JC, Tsuboi T. (他10名)
    • Journal Title

      Cell Host Microbe

      Volume: 11 Issue: 1 Pages: 7-18

    • DOI

      10.1016/j.chom.2011.11.011

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeting sialic acid dependent and independent pathways of invasion in Plasmodium falciparum2012

    • Author(s)
      Ord RL, Rodriguez M, Yamasaki T, Takeo S, Tsuboi T, Lobo CA
    • Journal Title

      PLoS One

      Volume: 7 Issue: 1 Pages: e30251-e30251

    • DOI

      10.1371/journal.pone.0030251

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biosynthesis, localisation and macromolecular arrangement of the Plasmodium falciparum translocon of exported proteins; PTEX2012

    • Author(s)
      Bullen HE, Torii M, Tsuboi T. (他11名)
    • Journal Title

      J Biol Chem

      Volume: 287 Issue: 11 Pages: 7871-7884

    • DOI

      10.1074/jbc.m111.328591

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Targeting sialic acid dependent and independent pathways of invasion in Plasmodium falciparum.2012

    • Author(s)
      Ord RL
    • Journal Title

      PLoS One

      Volume: 7

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PfSET10, a Plasmodium falciparum methyltransferase, maintains the active var gene in a poised state during parasite division.2012

    • Author(s)
      Volz JC
    • Journal Title

      Cell Host and Microbe

      Volume: 11 Pages: 7-18

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New insights into acquisition, boosting and longevity of immunity to malaria in pregnant women.2012

    • Author(s)
      Fowkes FJ
    • Journal Title

      Journal of Infectious Diseases

      Volume: 206 Pages: 1612-1621

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of GAMA, a Plasmodium falciparum merozoite micronemal protein, as a novel blood-stage vaccine candidate antigen2011

    • Author(s)
      Arumugam TU, Takeo S, Torii M, Tsuboi T. (他13名)
    • Journal Title

      Infect Immun

      Volume: 79 Issue: 11 Pages: 4523-4532

    • DOI

      10.1128/iai.05412-11

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasmodium vivax : comparison of immunogenicity among proteins expressed in the cell-free systems of Escherichia coli and wheat germ by suspension array assays2011

    • Author(s)
      Rui E, Takeo S, Tsuboi T. (他4名)
    • Journal Title

      Malar J

      Volume: 10 Issue: 1 Pages: 192-192

    • DOI

      10.1186/1475-2875-10-192

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tricomponent immunopotentiating system as a novel molecular design strategy for malaria vaccine development2011

    • Author(s)
      T.Miyata
    • Journal Title

      Infect.Immun.

      Volume: 79 Issue: 10 Pages: 4260-4275

    • DOI

      10.1128/iai.05214-11

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] N-terminal pro-domain of Pfs230 synthesized using cell-free system is sufficient to induce the complement dependent malaria transmission-blocking activity2011

    • Author(s)
      Tachibana, et al
    • Journal Title

      Clin Vaccine Immunol

      Volume: 18 Issue: 8 Pages: 1343-1350

    • DOI

      10.1128/cvi.05104-11

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Worldwide sequence conservation of transmission-blocking vaccine candidate Pvs230 in Plasmodium vivax2011

    • Author(s)
      Doi, M, Mita, T
    • Journal Title

      Vaccine

      Volume: 29 Issue: 26 Pages: 4308-15

    • DOI

      10.1016/j.vaccine.2011.04.028

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plasmodial ortholog of Toxoplasma gondii rhoptry neck protein 3 is localized to the rhoptry body2011

    • Author(s)
      Ito D, Han ET, Takeo S, Thongkukiatkul A, Otsuki H, Torii M, Tsuboi T
    • Journal Title

      Parasitol Int

      Volume: 60 Issue: 2 Pages: 132-138

    • DOI

      10.1016/j.parint.2011.01.001

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Discovery of GAMA, a Plasmodium falciparum merozoite micronemal protein, as a novel blood-stage vaccine candidate antigen2011

    • Author(s)
      Arumugam TU
    • Journal Title

      Infection and Immunity

      Volume: 79 Pages: 4523-4532

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasmodium vivax gametocyte protein Pvs230 is a transmission-blocking vaccine candidate2011

    • Author(s)
      Tachibana M
    • Journal Title

      Vaccine

      Volume: 30 Pages: 1807-1812

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antibodies against a Plasmodium falciparum antigen PfMSPDBL1 inhibit merozoite invasion into human erythrocytes2011

    • Author(s)
      Sakamoto H
    • Journal Title

      Vaccine

      Volume: 30 Pages: 1972-1980

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] ポストゲノムマラリアワクチン研究の最前線2013

    • Author(s)
      坪井敬文
    • Organizer
      金沢大学薬学シンポジウム2013
    • Place of Presentation
      金沢市
    • Year and Date
      2013-11-26
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Invited
  • [Presentation] マラリアワクチン研究の最前2013

    • Author(s)
      坪井敬文
    • Organizer
      岡山大学医歯薬学総合研究科第1回異分野キャリアを持つ医療系生命科学研究者育成支援事業セミナー
    • Place of Presentation
      岡山市
    • Year and Date
      2013-02-14
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Discovery of novel blood-stage malaria vaccine candidates of Plasmodium falciparum using wheat germ cell-free system2013

    • Author(s)
      坪井敬文
    • Organizer
      第86回日本生化学会大会インターナショナルシンポジウム
    • Place of Presentation
      横浜市
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] PRECLINICAL PRIORITIZATION OF BLOOD-STAGE MALARIA VACCINE CANDIDATES OF PLASMODIUM FALCIPARUM USING WHEAT GERM CELL-FREE SYSTEM2013

    • Author(s)
      Tsuboi T
    • Organizer
      ASTMH 62nd Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Identification of novel malaria vaccine candidates using wheat germ cell-free system2012

    • Author(s)
      Tsuboi T
    • Organizer
      The 10th Matsuyama International Symposium on Cell-Free Sciences
    • Place of Presentation
      Matsuyama, Japan
    • Year and Date
      2012-09-25
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Post-genome discovery of novel malaria vaccine candidates using wheat germ cell-free system2012

    • Author(s)
      Tsuboi T
    • Organizer
      Special Lecture at the Papua New Guinea Institute of Medical Research
    • Place of Presentation
      Madang, Papua New Guinea
    • Year and Date
      2012-09-13
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Application of cell-free protein synthesis technology for production of malaria protein2012

    • Author(s)
      Tsuboi T
    • Organizer
      Cell-Free Protein Synthesis Workshop
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2012-06-25
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Post-genome discovery of novel malaria vaccine candidates using wheat germ cell-free system2011

    • Author(s)
      Tsuboi T
    • Organizer
      Special Lecture at the Centre National de Recherche et de Formation sur le Paludisme
    • Place of Presentation
      Ouagadougou, Burkina Faso
    • Year and Date
      2011-10-27
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Post-genome discovery of novel malaria vaccine candidates using wheat germ cell-free system2011

    • Author(s)
      Tsuboi T
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌市
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Post-genome discovery of novel malaria vaccine candidates using wheat germ cell-free system2011

    • Author(s)
      Tsuboi T
    • Organizer
      Special Lecture at the Papua New Guinea Institute of Medical Research
    • Place of Presentation
      Goroka, Papua New Guinea
    • Year and Date
      2011-08-29
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Application of cell-free protein synthesis technology for production of malaria protein.

    • Author(s)
      Tsuboi T
    • Organizer
      Cell-Free Protein Synthesis Workshop
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Remarks]

    • URL

      http://www.pros.ehime-u.ac.jp/index.php

    • Related Report
      2013 Final Research Report
  • [Remarks] 愛媛大学プロテオサイエンスセンター

    • URL

      http://www.pros.ehime-u.ac.jp/index.php

    • Related Report
      2013 Annual Research Report
  • [Remarks] 愛媛大学プロテオサイエンスセンター

    • URL

      http://www.pros.ehime-u.ac.jp/eng/section/02.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.ehime-u.ac.jp/~cellfree/proteomedical/malaria.html

    • Related Report
      2011 Annual Research Report

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Published: 2011-04-06   Modified: 2019-07-29  

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