Screening of novel malaria vaccine candidates with protective immune sera
Project/Area Number |
23406007
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 海外学術 |
Research Field |
Parasitology (including Sanitary zoology)
|
Research Institution | Ehime University |
Principal Investigator |
TSUBOI Takafumi 愛媛大学, プロテオサイエンスセンター, 教授 (00188616)
|
Co-Investigator(Kenkyū-buntansha) |
TAKEO Satoru 杏林大学, 医学部, 准教授 (40302666)
TAKASHIMA Eizo 愛媛大, プロテオサイエンスセンター, 准教授 (50366762)
|
Project Period (FY) |
2011-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
|
Keywords | 原虫 / マラリア / ワクチン / プロテインアレイ / パプアニューギニア / 国際研究者交流 / 感染症 / バイオテクノロジー / ゲノム |
Research Abstract |
To identify novel malaria vaccine candidates, we first obtained serum samples from the children enrolled in the malaria cohort study in Papua New Guinean. We then established a micro-scale immunoscreening system for high-throughput detection of antigen-antibody reaction. Genome-widely expressed 1,900 parasite proteins by the wheat germ cell-free system covering one-third of the parasite genome were screened by using the immunoscreening system. As a result of the preliminary screening, we identified 650 immunoreactive proteins with the malaria immune serum samples which we previously had enough amount. The result suggests that this screening system would be effective to discover novel malaria vaccine candidates by using Papua New Guinean serum samples.
|
Report
(4 results)
Research Products
(40 results)
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[Journal Article] A small molecule glycosaminoglycan mimetic blocks Plasmodium invasion of the mosquito midgut.2013
Author(s)
Mathias DK, Pastrana-Mena R, Ranucci E, Ferruti P, de Beaumont C, Ortega C, Staples GO, Zaia J, Takashima E, Tsuboi T, Verotta L, Dinglasan RR
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Journal Title
PLoS Pathogens
Volume: 9
Issue: 11
Pages: e1003757-e1003757
DOI
Related Report
Peer Reviewed
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[Journal Article] RALP1 is a rhoptry neck erythrocyte-binding protein of Plasmodium falciparum merozoites and a potential blood-stage vaccine candidate antigen2013
Author(s)
Ito D, Hasegawa T, Miura K, Yamasaki T, Arumugam TU, Thongkukiatkul A, Takeo S, Takashima E, Sattabongkot J, Han ET, Long CA, Torii M, *Tsuboi
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Journal Title
Infect Immun
Volume: 81
Issue: 11
Pages: 4290-4298
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification and prioritization of merozoite antigens as targets of protective human immunity to Plasmodium falciparum malaria for vaccine and biomarker development.2013
Author(s)
Richards JS, Arumugam TU, Reiling L, Healer J, Hodder AN, Fowkes FJI, Cross N, Langer C, Takeo S, Uboldi AD, Thompson JK, Gilson PR, Coppel RL, Siba PM, King CL, Torii M, Chitnis CE, Narum DL, Mueller I, Crabb BS, Cowman AF, Tsuboi T, Beeson JG.
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Journal Title
Journal of Immunology
Volume: 191
Issue: 2
Pages: 795-809
DOI
Related Report
Peer Reviewed
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[Journal Article] The Plasmodium vivax merozoite surface protein 1 paralog is a novel erythrocyte-binding ligand of P. vivax.2013
Author(s)
Cheng Y, Wang Y, Ito D, Kong DH, Ha KS, Chen JH, Lu F, Li J, Wang B, Takashima E, Sattabongkot J, Tsuboi T, Han ET.
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Journal Title
Infection and Immunity
Volume: 81
Issue: 5
Pages: 1585-1595
DOI
Related Report
Peer Reviewed
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