The genomic epidemiology and immunological cohort study on amoebiasis in South Asia

• Project/Area Number: 23406009
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Nagasaki University
• Principal Investigator: HAMANO Shinjiro 長崎大学, 熱帯医学研究所, 教授 (70294915)
• Co-Investigator(Renkei-kenkyūsha): KATO Kentaro 長崎大学, 熱帯医学研究所, 助教 (50508885) ; MIICHI Fumika 佐賀大学, 医学部, 助教 (70576818)
• Project Period (FY): 2011-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 赤痢アメーバ / 新生児 / 乳児 / コホート研究 / Entamoeba histolytica / Entamoeba moshkovskii / 南アジア / バングラディシュ / バングラデシュ / 国際研究者交流 / 感染症 / 下痢症 / 赤痢アメーバ症 / ゲノム疫学

Research Abstract

In Mirpur, Dhaka, Bangladesh, natural exposure to Entamoeba spp. in infancy was prospectively observed. Approxymately, 80% of children had been infected with Entamoeba histolytica by age two years, with around 20% having suffered from E. histolytica. Furthermore, newly acquired E. moshkovskii infection was associated with diarrhea. Genetic analysis revealed that E. moshkovskii isolates infecting children were genetically heterogeneous, as evidenced by PCR typing of tRNA locus R-R. It will be important in future studies of the potential pathogenicity of E. moshkovskii to take into account this heterogeneity, as for the case of E. histolytica, not every genotype is equally capable of causing disease.

Research Products

• [Journal Article] Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice (2013)
  • Author(s): Shimokawa, C., Culleton, R., Imai, T., Suzue, K., Hirai, M., Taniguchi, T., Kobayashi, S., Hisaeda, H., Hamano, S
  • Journal Title: PLoS One Volume: 8(11) Issue: 11 Pages: e82025-e82025
  • DOI: 10.1371/journal.pone.0082025
  • NAID: 120006986684
  • Peer Reviewed
• [Journal Article] Entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice (2012)
  • Author(s): Shimokawa, C., Kabir, M., Taniuchi, M., Mondal, D., Kobayashi, S., Ali, I.K., Sobuz, S., Senba, M., Houpt, E., Haque, R., Petri, W.A., Hamano, S
  • Journal Title: J. Infect. Dis Volume: 206(5) Pages: 744-751
  • Peer Reviewed
• [Journal Article] Entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice. (2012)
  • Author(s): Shimokawa C, Kabir M, Taniuchi M, Mondal D, Kobayashi S, Ali IK, Sobuz SU, Senba M, Houpt E, Haque R, Petri WA Jr, Hamano S
  • Journal Title: J Infect Dis Volume: 206 Issue: 5 Pages: 744-751
  • DOI: 10.1093/infdis/jis414
  • Peer Reviewed
• [Presentation] Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice (2014)
  • Author(s): Shinjiro Hamano and Chikako Shimokawa
  • Organizer: US-Japan panel meeting
  • Place of Presentation: Dhaka, Bangladesh
  • Year and Date: 2014-02-11
• [Presentation] Intestinal inflammatory mediated clearance of Entamoeba moshkovskii isdependent on IFN-γ (2013)
  • Author(s): Shinjiro Hamano and Chikako Shimokawa
  • Organizer: 第12回あわじしま感染症免疫フォーラム
  • Place of Presentation: 兵庫
  • Year and Date: 2013-09-12
• [Presentation] Intestinal inflammatory mediated clearance of Entamoeba moshkovskii is dependent on IFN-γ. Forum Cheju 16 (2013)
  • Author(s): Shinjiro Hamano and Chikako Shimokawa
  • Organizer: Seoul National University
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2013-08-31
• [Presentation] Entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice (2013)
  • Author(s): Shimokawa C, Hamano S
  • Organizer: InternationalWorkshop on Enteric Protozoan infections
  • Place of Presentation: 東京
  • Year and Date: 2013-02-22
• [Presentation] Double-edged effects of IFN-・in amoeba infection of mice (2013)
  • Author(s): 下川周子, 小林正規, 千馬正敬, 鈴江一友, 平井誠, 今井孝, 谷口委代, 久枝一, 濱野真二郎
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京
• [Presentation] Entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice (2013)
  • Author(s): Shimokawa C, Kabir M, Taniuchi M, Mondal D, Kobayashi S, Ali IK, Sobuz SU, Senba M, Houpt E, Haque R, Petri WA Jr, Hamano S
  • Organizer: The XVII Amibiasis Meeting
  • Place of Presentation: Merida, Mexico
• [Presentation] Entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice. (2013)
  • Author(s): Shimokawa C, Hamano S
  • Organizer: International Workshop on Enteric Protozoan infections.
  • Place of Presentation: 国立感染症研究所（東京）
  • Invited
• [Presentation] Entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice. (2013)
  • Author(s): 5. Shimokawa C, Kabir M, Taniuchi M, Mondal D, Kobayashi S, Ali IK, Sobuz SU, Senba M, Houpt E, Haque R, Petri WA Jr, Hamano S
  • Organizer: The XVII Amibiasis Meeting
  • Place of Presentation: Hotel Fiesta Americana （Merida, Mexico）
• [Presentation] Double-edged effects of IFN-・ in mice infected with Entamoeba moshkovskii (2012)
  • Author(s): Chikako Shimokawa, Shinjiro Hamano
  • Organizer: 第81回日本寄生虫学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2012-03-23
• [Presentation] Entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice (2012)
  • Author(s): Chikako Shimokawa, Mamum Kabir, Mami Taniuchi, Dinesh Mondal, Seiki Kobayashi, Ibne Karim M. Ali, Masachika Senba, Eric Hoput, Haque Rashidul, William A. Petri Jr., Shinjiro Hamano
  • Organizer: 第81回日本寄生虫学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2012-03-23
• [Presentation] Double-edged effects of IFN-・ in mice infected with Entamoeba moshkovskii (2012)
  • Author(s): Chikako Shimokawa, Shinjiro Hamano
  • Organizer: 第5回寄生虫感染免疫研究会
  • Place of Presentation: 大阪
  • Year and Date: 2012-03-02
• [Presentation] Entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice (2012)
  • Author(s): Shinjiro Hamano, Chikako Shimokawa
  • Organizer: 第5回寄生虫感染免疫研究会
  • Place of Presentation: 大阪
  • Year and Date: 2012-03-01
• [Presentation] IFN-・ is necessary for clearance of pathogenic amoebae from the intestine of mice (2012)
  • Author(s): Chikako Shimokawa, Shinjiro Hamano
  • Organizer: 第6回長崎熱帯病新興感染症シンポジウム
  • Place of Presentation: 長崎
• [Presentation] IFN-・ is necessary for clearance of pathogenic amoebae from the intestine of mice (2012)
  • Author(s): Chikako Shimokawa, Shinjiro Hamano
  • Organizer: 第41回日本免疫学会
  • Place of Presentation: 大阪
• [Presentation] IFN-・ up-regulates NKG2D expression on intestinal intra epithelial cells in mice infected with amoebic parasites (2012)
  • Author(s): Chikako Shimokawa, Masachika Senba, Shinjiro Hamano
  • Organizer: 第11回あわじしま感染症免疫フォーラム
  • Place of Presentation: 兵庫
• [Presentation] IFN-gamma up-regulates NKG2D expression on intestinal intra epithelial cells in mice infected with amoebic parasites. (2012)
  • Author(s): Chikako Shimokawa, Masachika Senba, Shinjiro Hamano
  • Organizer: 第11回 あわじしま感染症免疫フォーラム
  • Place of Presentation: あわじ夢舞台（兵庫）
• [Presentation] IFN-gamma is necessary for clearance of pathogenic amoebae from the intestine of mice. (2012)
  • Author(s): Chikako Shimokawa, Shinjiro Hamano:
  • Organizer: 第41回 日本免疫学会
  • Place of Presentation: 神戸国際会議場・展示場（兵庫）
• [Presentation] IFN-gamma is necessary for clearance of pathogenic amoebae from the intestine of mice. (2012)
  • Author(s): Chikako Shimokawa, Shinjiro Hamano
  • Organizer: 第6回 長崎熱帯病新興感染症シンポジウム
  • Place of Presentation: 長崎大学（長崎）
• [Presentation] Entamoeba moshkovskii is associated with diarrhea in infants and causes diarrhea and colitis in mice. (2012)
  • Author(s): Chikako Shimokawa, Mamum Kabir, Mami Taniuchi, Dinesh Mondal, Seiki Kobayashi, Ibne Karim M. Ali, Masachika Senba, Eric Hoput, Haque Rashidul, William A. Petri Jr., Shinjiro Hamano
  • Organizer: 第81回 日本寄生虫学会総会
  • Place of Presentation: 兵庫医科大学（兵庫）
• [Presentation] A comparative study on glycosylation patterns of mucus glycoprotein between CBA and C57BL/6 mice (2011)
  • Author(s): Kentaro Kato, Shinjiro Hamano
  • Organizer: 第80回日本寄生虫学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-07-17
• [Presentation] Pathogenicity of Entamoeba moshkovskii in human and mice (2011)
  • Author(s): Chikako Shimokawa, Seiki Kobayashi, Masachika Senba, Eric Houpt, William A Petri Jr., Rashidul Haque, Shinjiro Hamano
  • Organizer: 第80回日本寄生虫学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-07-17
• [Presentation] Entamoeba moshkovskii is pathogenic and causes intestinal symptoms (2011)
  • Author(s): Chikako Shimokawa, Mamum Kabir, Mami Taniuchi, Dinesh Mondal, Seiki Kobayashi, Ibne Karim M. Ali, Masachika Senba, Eric Hoput, Haque Rashidul, William A. Petri Jr., Shinjiro Hamano
  • Organizer: The 60th annual meeting of American society of tropical medicine and hygiene
  • Place of Presentation: Philadelphia
• [Presentation] IFN-・-mediated protection against Entamoeba moshkovskii infection (2011)
  • Author(s): Chikako Shimokawa, Shinjiro Hamano
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
• [Presentation] Pathogenicity of Entamoeba moshkovskii in human and mice. (2011)
  • Author(s): Chikako Shimokawa, Seiki Kobayashi, Masachika Senba, Eric Houpt, William A Petri Jr., Rashidul Haque, Shinjiro Hamano
  • Organizer: 第80回日本寄生虫学会大会
  • Place of Presentation: 東京慈恵会医科大学（東京）
• [Presentation] ミニシンポジウム『熱帯医学からの発信』、病原性アメーバの再発見： -フィールド・ラボ双方向からのアプローチ (2011)
  • Author(s): 濱野真二郎
  • Organizer: 第52回日本熱帯医学会
  • Place of Presentation: 東京大学（東京）
  • Invited
• [Presentation] IFN-gamma-mediated protection against Entamoeba moshkovskii infection. (2011)
  • Author(s): Chikako Shimokawa, , Shinjiro Hamano
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] Entamoeba moshkovskii is pathogenic and causes intestinal symptoms. (2011)
  • Author(s): Chikako Shimokawa, Mamum Kabir, Mami Taniuchi, Dinesh Mondal, Seiki Kobayashi, Ibne Karim M. Ali, Masachika Senba, Eric Hoput, Haque Rashidul, William A. Petri Jr., Shinjiro Hamano:
  • Organizer: The 60th annual meeting of American society of tropical medicine and hygiene
  • Place of Presentation: Philadelphia Marriott Downtown (Philadelphia, PA, USA)
• [Presentation] Intestinal inflammatory mediated clearance of Entamoeba moshkovskii is dependent on IFN-g
  • Author(s): Shinjiro Hamano and Chikako Shimokawa
  • Organizer: Forum Cheju 16
  • Place of Presentation: Seoul National University （韓国）
• [Presentation] Intestinal inflammatory mediated clearance of Entamoeba moshkovskii isdependent on IFN-g
  • Author(s): Shinjiro Hamano and Chikako Shimokawa
  • Organizer: 第12回 あわじしま感染症免疫フォーラム
  • Place of Presentation: あわじ夢舞台国際会議場 （兵庫県）
• [Presentation] Species-specific immunity induced by infection with Entamoeba histolytica and Entamoeba moshkovskii in mice
  • Author(s): Shinjiro Hamano and Chikako Shimokawa
  • Organizer: US-Japan panel meeting
  • Place of Presentation: ICDDR, B. （Dhaka, Bangladesh）
  • Invited
• [Book] アメーバ症, 今日の診断指針第7版 (2014)
  • Author(s): 濱野真二郎
  • Publisher: 医学書院(印刷中)
• [Book] アメーバ症, 今日の治療指針2014 (2014)
  • Author(s): 濱野真二郎
  • Total Pages: 255
  • Publisher: 医学書院
• [Book] Amoebiasis in "Feigin, Cherry, Demmler, Kaplan : Textbook of Pediatric Infectious Disease, 7th edition" (2013)
  • Author(s): Hamano, S. andWilliam A. Petri Jr
  • Publisher: Elsevier
• [Book] Amoebiasis in “Feigin, Cherry, Demmler, Kaplan: Textbook of Pediatric Infectious Disease, 7th edition” (2013)
  • Author(s): Hamano, S. and William A. Petri Jr.
  • Publisher: Elsevier
• [Book] 化学療法の領域 (2011)
  • Author(s): 原田倫世、濱野真二郎
  • Publisher: アメーバ赤痢やクリプトスポリジウム症の現状と最新の知見
• [Book] 感染症事典 (2011)
  • Author(s): 下川周子・濱野真二郎
  • Publisher: ジアルジア症、自由生活性アメーバ症、赤痢アメーバ症
• [Remarks]
  • URL: http://www.tm.nagasaki-u.ac.jp/nekken/research/parasitology.html
• [Remarks] 長崎大学・熱帯医学研究所・寄生虫学分野
  • URL: http://www.tm.nagasaki-u.ac.jp/nekken/research/parasitology.html