Study of the functional role of CYP511 in myelination and remyelination.
| Project/Area Number |
23500404
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Tokushima Bunri University |
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Principal Investigator |
SONG Si-Young 徳島文理大学, 大学共同利用機関等の部局等, 教授 (00399693)
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| Co-Investigator(Kenkyū-buntansha) |
NAKASHIMA Kentaro 徳島文理大学, 大学共同利用機関等の部局等, 助手 (20449911)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | LDM / CYP51 / オリゴデンドログリア / シュワン細胞 / 髄鞘形成 / 脱髄 / 髄鞘再生 / cuprizone / 髄鞘 / proteolipid protein / トランスジェニックマウス / 脂質代謝 / SNP / 酵素活性 / CO差還元 spectrum |
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| Research Abstract |
LDM is the only cytochrome P450 enzyme that is involved in cholesterol biosynthesis and is expressed in central and peripheral myelin-forming cells, oligodendroglia and Schwann cell. Immunohistochemical analysis and Western blot analysis combined with laser capture microdissection using anti-LDM antibody revealed that LDM-immunoreactivity (IR) reached its peak at the stage of myelination, postnatal 2-3 weeks. The same kind of analyses indicated that LDM-IR in the white matter increased during the processes of remyelination following demyelination induced in mice by feeding diet containing cuprizone that injures oligodendroglia. To examine the possibility to treat demyelinating disorder by the upregulation of LDM, transgenic mice were raised using PLP gene-promoter, which express high level of LDM specifically in oligodendroglia.
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Report
(4 results)
Research Products
(8 results)
