Functions of Toll family proteins in synaptic development and maintenance

• Project/Area Number: 23500405
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: National Institute of Genetics
• Principal Investigator: SUZUKI Emiko 国立遺伝学研究所, 構造遺伝学研究センター, 准教授 (20173891)
• Co-Investigator(Renkei-kenkyūsha): KURUSU Mitsuhiko 国立遺伝学研究所, 構造遺伝学研究センター, 助教 (50413985)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ショウジョウバエ / 神経筋接合 / シナプス / Toll / 神経-筋接合 / グルタミン酸受容体

Outline of Final Research Achievements

Various plasma membrane proteins play important roles in the processes of synaptogenesis, as well as synaptic maintenance and plasticity. We are exploring the key functions of such proteins using Drosophila neuromuscular junctions (NMJ). In the present study, we focused on Toll family proteins. By the phenotype analyses of genetic overexpression or loss-of-function mutation, we found that Toll-7 functions as a negative regulator of NMJ development and maintenance. On the other hand, we have not observed the impairment of synaptic morphology by genetic RNAi of Spz family proteins, the putative ligand of Toll family proteins.

Research Products

• [Journal Article] Downregulation of Centaurin gamma1A increases synaptic transmission at Drosophila larval neuromuscular junctions (2014)
  • Author(s): Homma, M.、 Nagashima, S., Fukuda, T., Yanagi, S., Miyakawa, H., Suzuki, E., Morimoto, T.
  • Journal Title: Europian Journal of Neuroscience Volume: VOL40 Issue: 8 Pages: 3158-3170
  • DOI: 10.1111/ejn.12681
  • Peer Reviewed
• [Journal Article] 神経分化に伴うN-カドヘリンの細胞内分布と機能の変化 (2013)
  • Author(s): 鈴木えみ子、来栖光彦
  • Journal Title: 生体の科学 Volume: 64 Pages: 249-253
• [Journal Article] Developmental changes in expression, subcellular distribution, and function of Drosophila N-cadherin, guided by a cell-intrinsic program during neuronal differentiation. (2012)
  • Author(s): Kurusu, M.,Katsuki, T., Zinn, K., Suzuki, E.
  • Journal Title: Developmental Biology Volume: 366 Issue: 2 Pages: 204-217
  • DOI: 10.1016/j.ydbio.2012.04.006
  • Peer Reviewed
• [Presentation] Molecular remodeling of the presynaptic active zone of Droosphila photoreceptors via an activity-dependent feedback signal (2014)
  • Author(s): 杉江 淳, 羽毛田(鈴木) 聡子, 鈴木 えみ子, Tavosanis Gaia, 鈴木 崇之
  • Organizer: 第３７回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜 横浜市
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] 神経活動依存的なフィードバックシグナルによる、ショウジョウバエ視神経細胞の前シナプスの活動部位における分子的再構成 (2014)
  • Author(s): 鈴木 崇之, 杉江 淳, 羽毛田 聡子, 鈴木 えみ子, Tavasanis Gaia
  • Organizer: 第３７回日本神経科学大会
  • Place of Presentation: パシフィコ横浜 横浜市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Activity-dependent synaptic remodeling in the Drosophila photoreceptor neurons (2014)
  • Author(s): 杉江 淳, 羽毛田-鈴木 聡子, 鈴木 えみ子, Tavosanis Gaia, 鈴木 崇之
  • Organizer: 第11回日本ショウジョウバエ研究会
  • Place of Presentation: 金沢歌劇座 金沢市
  • Year and Date: 2014-06-04 – 2014-06-06
• [Presentation] ショウジョウバエFGFシグナルによる後シナプスグルタミン酸受容体サブユニットの発現制御 (2013)
  • Author(s): 小林百合, 来栖光彦, 鈴木えみ子
  • Organizer: 第３６回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランドホール（神戸市中央区）
• [Presentation] ショウジョウバエFGFシグナルによる後シナプス分化の制御 (2012)
  • Author(s): 小林百合、鈴木えみ子、来栖光彦
  • Organizer: 第３５回日本神経科学大会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
• [Presentation] FGF signaling regulates post synaptic development in the Drosophila neuromuscular junction. (2012)
  • Author(s): 小林百合、来栖光彦、鈴木えみ子
  • Organizer: The 10th Japanese Drosophila Research Conference
  • Place of Presentation: 東京慈恵会医科大学（東京都）
• [Remarks] 鈴木えみ子研究室ホームページ
  • URL: http://www.nig.ac.jp/labs/GenNetwk/kairo-hp/home/index.html
• [Remarks] 神経分化に伴う細胞接着分子N-cadherinの機能変化とその発現制御
  • URL: http://www.nig.ac.jp/Research-Highlights/992/1067.html