Live imaging analysis of molecular mechanisms underlying the neuronal morphogenesis in the developing cerebral cortex
| Project/Area Number |
23500410
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Chubu University (2014)
Nagoya University (2011-2013) |
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Principal Investigator |
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ニューロン / 細胞移動 / 軸索 / 樹状突起 / 大脳皮質形成 / 大脳皮質 / 形態形成 / 遺伝子発現 / 神経科学 |
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| Outline of Final Research Achievements |
Forced expression of wild type or mutant molecules is a powerful tool for analyzing protein function in cells and it is important to restrict gene expression in specific cell type such as neurons. Although many proteins have been implicated in regulation of neuronal migration and polarization in the developing cerebral cortex, most described phenotypes were based on forced expression using constitutively active transcriptional promoters, which are also active in progenitors. This makes it difficult to analyze neuronal phenotypes in later stage. To develop a reliable expression system to validate the function of proteins in neurons, we have analyzed usability of transcriptional promoters in cortical neurons and progenitors. Expression patterns of several promoters including, EF-1α, and CMV-β-actin chimeric, Tα1, NeuroD suggested that Tα1-driven strong neuronal expression triggered by NeuroD-Cre is a powerful tool for neuron-restricted expression of ectopic genes.
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Report
(5 results)
Research Products
(29 results)
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[Journal Article] Pioneering Axons Regulate Neuronal Polarization in the Developing Cerebral Cortex.2014
Author(s)
Namba T, Kibe Y, Funahashi Y, Nakamuta S, Takano T, Ueno T, Shimada A, Kozawa S, Okamoto M, Shimoda Y, Oda K, Wada Y, Masuda T, Sakakibara A, Igarashi M, Miyata T, F-S Catherine, Takeuchi K, Kaibuchi K.
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Journal Title
Neuron
Volume: 81(4)
Issue: 4
Pages: 814-829
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Shootin1 acts in concert with KIF20B to promote polarization of migrating neurons.2013
Author(s)
Sapir, T., Levy, T., Sakakibara, A., Rabinkov, A., Miyata, T., Reiner, O.
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Journal Title
Journal of Neuroscience
Volume: 33
Issue: 29
Pages: 11932-11948
DOI
Related Report
Peer Reviewed
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[Journal Article] TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding.2013
Author(s)
Okamoto M, Namba T, Shinoda T, Kondo T, Watanabe T, Inoue Y, Takeuchi K, Enomoto Y, Ota K, Oda K, Wada Y, Sagou K, Saito K, Sakakibara A, Kawaguchi A, Nakajima K, Adachi T, Fujimori T, Ueda M, Hayashi S, Kaibuchi K, Miyata T
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Journal Title
Nature Neuroscience
Volume: 16
Issue: 11
Pages: 1556-66
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration2013
Author(s)
Xie, M.-J., Yagi, H., Kuroda, K., Wang C.-C., Komada, M., Zhao, H., Sakakibara, A., Miyata, T., Nagata, K., Oka, Y., Iguchi, T. and Sato, M.
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Journal Title
Cerebral Cortex
Volume: (in press)
Issue: 6
Pages: 1410-1423
DOI
Related Report
Peer Reviewed
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[Journal Article] Dynamics of centrosome translocation and microtubule organization in neocortical neurons during distinct modes of polarization2013
Author(s)
Sakakibara, A., Sato, T., Ando, R., Noguchi, N., Masaoka, M., Miyata, T
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Journal Title
Cereb. Cortex
Volume: -
Issue: 5
Pages: 1301-1310
DOI
Related Report
Peer Reviewed
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