| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Forced expression of wild type or mutant molecules is a powerful tool for analyzing protein function in cells and it is important to restrict gene expression in specific cell type such as neurons. Although many proteins have been implicated in regulation of neuronal migration and polarization in the developing cerebral cortex, most described phenotypes were based on forced expression using constitutively active transcriptional promoters, which are also active in progenitors. This makes it difficult to analyze neuronal phenotypes in later stage. To develop a reliable expression system to validate the function of proteins in neurons, we have analyzed usability of transcriptional promoters in cortical neurons and progenitors. Expression patterns of several promoters including, EF-1α, and CMV-β-actin chimeric, Tα1, NeuroD suggested that Tα1-driven strong neuronal expression triggered by NeuroD-Cre is a powerful tool for neuron-restricted expression of ectopic genes.
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