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Live imaging analysis of molecular mechanisms underlying the neuronal morphogenesis in the developing cerebral cortex

Research Project

Project/Area Number 23500410
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionChubu University (2014)
Nagoya University (2011-2013)

Principal Investigator

SAKAKIBARA Akira  中部大学, 生命健康科学部, 准教授 (20510217)

Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsニューロン / 細胞移動 / 軸索 / 樹状突起 / 大脳皮質形成 / 大脳皮質 / 形態形成 / 遺伝子発現 / 神経科学
Outline of Final Research Achievements

Forced expression of wild type or mutant molecules is a powerful tool for analyzing protein function in cells and it is important to restrict gene expression in specific cell type such as neurons. Although many proteins have been implicated in regulation of neuronal migration and polarization in the developing cerebral cortex, most described phenotypes were based on forced expression using constitutively active transcriptional promoters, which are also active in progenitors. This makes it difficult to analyze neuronal phenotypes in later stage. To develop a reliable expression system to validate the function of proteins in neurons, we have analyzed usability of transcriptional promoters in cortical neurons and progenitors. Expression patterns of several promoters including, EF-1α, and CMV-β-actin chimeric, Tα1, NeuroD suggested that Tα1-driven strong neuronal expression triggered by NeuroD-Cre is a powerful tool for neuron-restricted expression of ectopic genes.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (29 results)

All 2015 2014 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (19 results) (of which Invited: 3 results) Remarks (1 results)

  • [Journal Article] Neuronal polarization in the developing cerebral cortex2015

    • Author(s)
      Akira Sakakibara
    • Journal Title

      Front. Neurosci.

      Volume: 9 Pages: 116-116

    • DOI

      10.3389/fnins.2015.00116

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Pioneering Axons Regulate Neuronal Polarization in the Developing Cerebral Cortex.2014

    • Author(s)
      Namba T, Kibe Y, Funahashi Y, Nakamuta S, Takano T, Ueno T, Shimada A, Kozawa S, Okamoto M, Shimoda Y, Oda K, Wada Y, Masuda T, Sakakibara A, Igarashi M, Miyata T, F-S Catherine, Takeuchi K, Kaibuchi K.
    • Journal Title

      Neuron

      Volume: 81(4) Issue: 4 Pages: 814-829

    • DOI

      10.1016/j.neuron.2013.12.015

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microtubule dynamics in neuronal morphogenesis2013

    • Author(s)
      Akira Sakakibara
    • Journal Title

      Open Biol.

      Volume: 3 Issue: 7 Pages: 130061-130061

    • DOI

      10.1098/rsob.130061

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Shootin1 acts in concert with KIF20B to promote polarization of migrating neurons.2013

    • Author(s)
      Sapir, T., Levy, T., Sakakibara, A., Rabinkov, A., Miyata, T., Reiner, O.
    • Journal Title

      Journal of Neuroscience

      Volume: 33 Issue: 29 Pages: 11932-11948

    • DOI

      10.1523/jneurosci.5425-12.2013

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding.2013

    • Author(s)
      Okamoto M, Namba T, Shinoda T, Kondo T, Watanabe T, Inoue Y, Takeuchi K, Enomoto Y, Ota K, Oda K, Wada Y, Sagou K, Saito K, Sakakibara A, Kawaguchi A, Nakajima K, Adachi T, Fujimori T, Ueda M, Hayashi S, Kaibuchi K, Miyata T
    • Journal Title

      Nature Neuroscience

      Volume: 16 Issue: 11 Pages: 1556-66

    • DOI

      10.1038/nn.3525

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] WAVE2-Abi2 complex controls growth cone activity and regulates the multipolar-bipolar transition as well as the initiation of glia-guided migration2013

    • Author(s)
      Xie, M.-J., Yagi, H., Kuroda, K., Wang C.-C., Komada, M., Zhao, H., Sakakibara, A., Miyata, T., Nagata, K., Oka, Y., Iguchi, T. and Sato, M.
    • Journal Title

      Cerebral Cortex

      Volume: (in press) Issue: 6 Pages: 1410-1423

    • DOI

      10.1093/cercor/bhs123

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dynamics of centrosome translocation and microtubule organization in neocortical neurons during distinct modes of polarization2013

    • Author(s)
      Sakakibara, A., Sato, T., Ando, R., Noguchi, N., Masaoka, M., Miyata, T
    • Journal Title

      Cereb. Cortex

      Volume: - Issue: 5 Pages: 1301-1310

    • DOI

      10.1093/cercor/bhs411

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reelin-dependent ApoER2 downregulation uncouples newborn neurons from progenitor cells2012

    • Author(s)
      F. Javier Perez-Martinez
    • Journal Title

      Biol. Open

      Volume: 1 Issue: 12 Pages: 1258-1263

    • DOI

      10.1242/bio.20122816

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Local Application of Neurotrophins Specifies Axons Through Inositol 1,4,5-Trisphosphate, Calcium, and Ca^,<2+>/Calmodulin-Dependent Protein Kinases2011

    • Author(s)
      Nakamuta S., et al
    • Journal Title

      Science Signaling

      Volume: 4 Issue: 199

    • DOI

      10.1126/scisignal.2002011

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Rear accumulation of actin underlies nucleus-centrosome inversion type of nucleokinesis in migrating neocortical neurons2014

    • Author(s)
      Akira Sakakibara
    • Organizer
      The 2014 ASCB/IFCB meeting
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2014-12-07 – 2014-12-10
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大脳皮質ニューロンの移動と極性化における微小管の機能2014

    • Author(s)
      榊原明
    • Organizer
      第66回 日本細胞生物学会大会
    • Place of Presentation
      奈良県新公会堂(奈良市)
    • Year and Date
      2014-06-11 – 2014-06-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大脳皮質ニューロンの移動と極性化における細胞質微小管の機能2013

    • Author(s)
      榊原明
    • Organizer
      第65回日本細胞生物学会大会
    • Place of Presentation
      名古屋、ウィンクあいち
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] 大脳皮質ニューロンの移動と極性化における細胞質微小管の機能2013

    • Author(s)
      榊原明
    • Organizer
      Neuro2013
    • Place of Presentation
      京都、国立京都国際会館
    • Related Report
      2013 Research-status Report
  • [Presentation] Microtubule regulation of neuronal migration and polarization in the developing mouse cerebrum2013

    • Author(s)
      Akira Sakakibara
    • Organizer
      EMBO2013
    • Place of Presentation
      Amsterdam, RAI, オランダ
    • Related Report
      2013 Research-status Report
  • [Presentation] 胎生期マウス大脳皮質ニューロン移動と極性化における微小管の機能2013

    • Author(s)
      榊原明
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸、神戸ポートアイランド
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Microtubule regulation of neuronal migration and polarization in the developing mouse cerebrum2013

    • Author(s)
      Akira Sakakibara
    • Organizer
      ASCB2013
    • Place of Presentation
      New Orleans, convention center, アメリカ
    • Related Report
      2013 Research-status Report
  • [Presentation] Dynamics of centrosome and microtubule plus ends in neocortical neurons during migration and axon formation in situ2012

    • Author(s)
      Akira Sakakibara
    • Organizer
      EMBO Conference Series Microtubules: Structure, Regulation and Functions
    • Place of Presentation
      Heidelberg, Germany
    • Related Report
      2012 Research-status Report
  • [Presentation] ニューロン移動と極性化における細胞質微小管の機能2012

    • Author(s)
      榊原明
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 小脳プルキンエ細胞を生み出す前駆細胞の挙動についての研究2012

    • Author(s)
      宮田卓樹
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] Microtubule dynamics in neocortical neurons during distinct modes of migration and polarization in the developing mouse cerebrum2012

    • Author(s)
      Akira Sakakibara
    • Organizer
      The 4th EMBO meeting Nice
    • Place of Presentation
      Nice, France
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Microtubule dynamics in neocortical neurons during distinct modes of migration and polarization in the developing mouse cerebrum2012

    • Author(s)
      Akira Sakakibara
    • Organizer
      The 52th American Society for Cell Biology Annual Meeting
    • Place of Presentation
      San Francisco, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] Centrosome dynamics in neocortical pyramidal neurons during the transition of migration mode from multipolar to locomotion, and formation of axon-like fiber

    • Author(s)
      Akira Sakakibara
    • Organizer
      Cortical Development 2011
    • Place of Presentation
      Chania (Greece)
    • Related Report
      2011 Research-status Report
  • [Presentation] 大脳皮質錐体ニューロンの移動・極性化における微小管細胞骨格の機能

    • Author(s)
      榊原明
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2011 Research-status Report
  • [Presentation] 大脳皮質錐体ニューロンの移動と突起形成における微小管動態

    • Author(s)
      榊原明
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Related Report
      2011 Research-status Report
  • [Presentation] Function of centrosome and cytoplasmic microtubules in polarization of neocortical neurons during the 3D migration and process formation in situ

    • Author(s)
      Akira Sakakibara
    • Organizer
      The 26th Europian Cytoskeletal Forum Meeting
    • Place of Presentation
      Stresa (Italy)
    • Related Report
      2011 Research-status Report
  • [Presentation] Dynamics of centrosome and microtubule plus ends during migration and process formation in neocortical neurons in situ

    • Author(s)
      Akira Sakakibara
    • Organizer
      The 41th Annual Meeting of the Society for Neuroscience
    • Place of Presentation
      Washington DC (USA)
    • Related Report
      2011 Research-status Report
  • [Presentation] Function of centrosome and cytoplasmic microtubules in polarization of neocortical neurons during the 3D migration and process formation in situ

    • Author(s)
      榊原明
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川)
    • Related Report
      2011 Research-status Report
  • [Presentation] ニューロン移動と極性化における細胞質微小管の機能

    • Author(s)
      榊原明
    • Organizer
      第117回日本解剖学会総会・全国学術集会(招待講演)
    • Place of Presentation
      山梨大学(山梨)
    • Related Report
      2011 Research-status Report
  • [Remarks] 宮田研究室メンバー紹介ページ

    • URL

      http://www.takaki-miyata-lab.org/english/members/akira-sakakibara/

    • Related Report
      2012 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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