| Project/Area Number |
23500416
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Nara Medical University |
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Principal Investigator |
TATSUMI Kouko 奈良県立医科大学, 医学部, 准教授 (90208033)
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| Co-Investigator(Kenkyū-buntansha) |
WANAKA Akio 奈良県立医科大学, 医・第2解剖学講座, 教授 (90210989)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | アストロサイト / Olig2 / 大脳基底核 / 視床下核 / パーキンソン氏病 / olig2 / 抑制性ニューロン / ドパミン / パーキンソン病 / チャネルロドプシン / GFP |
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| Research Abstract |
In this study, we found that Olig2 positive cells constitute a subpopulation of astrocytes in the adult mouse forebrain and distribute specifically in the basal ganglia including subthalamic nucleus (STN) and the globus pallidus. It is well known that these two nuclei are target areas for deep brain stimulation (DBS) in Parkinson's disease. To know the property of Olig2-astrocyte in the area, we made a hemi-Parkinson's disease model using Olig2CreER: ROSA-GAP43-EGFP double transgenic mouse. Upon apomorphine injection, the mice exhibited rotation behaviors that reflected imbalance of dopaminergic activities. In this experimental paradigm, we found that Olig2-astrocyte had more complex bushy morphologies in the activated side of the basal ganglia, especially in the STN than in the contralateral side. These findings suggest that morphologies of astrocytes are closely related to neuronal activities in the basal ganglionic nuclei.
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