| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
We examined whether peripheral nerve injury affects on the phosphorylation of L1-CAM in the rat DRG and spinal cord using a phospho-specific L1-CAM (Ser1181) antibody. Expression of phosphorylated L1-CAM was examined in rats (Sprague-Dawley, 250 g) that received tibial and common peroneal nerve ligation. Total and phosphorylated L1-CAM immunoreactivity (ir) were constitutively expressed in small un-myelinated DRG neurons. Nerve injury decreased total L1-CAM ir in the cytoplasm of somata in small neurons and increased it in cell membrane resulting to the formation of L1-CAM ir ring-like structures of in the injured small DRG neurons. Phosphorylated L1-CAM ir showed a dramatic decrease in the injured DRG. In dorsal horn, total L1-CAM showed the increase in the terminal of primary afferents horn. In contrast to the DRG, nerve injury increased the phosphorylated L1-CAM in the terminal of primary afferent in the dorsal horn .
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