The role of abnormal alpha-synuclein and its partners in synucleinopathy
Project/Area Number |
23500425
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Nerve anatomy/Neuropathology
|
Research Institution | Hirosaki University |
Principal Investigator |
TANJI kunikazu 弘前大学, 医学(系)研究科(研究院), 助教 (10271800)
|
Co-Investigator(Kenkyū-buntansha) |
WAKABAYASHI Koichi 弘前大学, 大学院・医学研究科, 教授 (50240768)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | タンパク質分解 / 異常蓄積 / 神経変性 / 神経変性疾患 / アルツハイマー病 / パーキンソン病 / レビー小体型認知症 / シナプス / 封入体 / ユビキチン / プロテアソーム / オートファジー |
Research Abstract |
Abnormal synuclein is deposited in the presynapses and cytoplasmic inclusions found in synucleinopathy such as Parkinsons disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy. Likewise, NUB1 is also accumulated in these specific regions together with abnormal synuclein. Although NUB1 can degrade synuclein expressed in cultured cells, it is not clear that NUB1 possesses this ability in vivo. We created transgenic mice expressing NUB1 and synuclein to investigate the role of NUB1 on abnormal synuclein deposition. Immunohistochemical and biochemical studies confirmed that NUB1 was expressed in the neurons. Unexpectedly, however, there were no alterations of abnormal synuclein deposition between mice expressing synuclein and synuclein/NUB1. These results suggest that NUB1 unlikely degrades synuclein in vivo.
|
Report
(4 results)
Research Products
(23 results)
-
-
[Journal Article] Carnosic acid suppresses the production of amyloid-beta 1-42 and 1-43 by inducing an alpha-secretase TACE/ADAM17 in U373MG human astrocytoma cells.2014
Author(s)
Yoshida H, Meng P, Matsumiya T, Tanji K, Hayakari R, Xing F, Wang L, Tsuruga K, Tanaka H, Mimura J, Kosaka K, Itoh K, Takahashi I, Imaizumi T
-
Journal Title
Neurosci Res
Volume: 79
Pages: 89-94
DOI
Related Report
Peer Reviewed
-
[Journal Article] ALS-associated protein FIG4 is localized in Pick and Lewy bodies, and neuronal nuclear inclusions in polyglutamine and intranuclear inclusion body diseases.2014
Author(s)
Kon T, Mori F, Yanji F, Miki Y, Toyoshima Y, Yoshida M, Sasaki H, Kakita A, Takahashi H, Wakabayashi K
-
Journal Title
Neuropathology
Volume: 34
Issue: 1
Pages: 19-26
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
[Presentation] Accumulation of autophagic adaptor protein, p62/sequestosome 1, in the brains of Alzheimer's disease2013
Author(s)
Kunikazu Tanji, Tomoh Matsumiya, Atsushi Maruyama, Saori Odagiri, Fumiaki Mori, Ken Itoh, Akiyoshi Kakita, Hitoshi Takahashi and Koichi Wakabayashi
Organizer
15th International Congress of Immunology
Place of Presentation
Milan, Italy
Related Report
-
-
-
[Presentation] Accumulation of autophagic adaptor protein, p62/sequestosome 1, in the brains of Alzheimer’s disease2013
Author(s)
Kunikazu Tanji, Tomoh Matsumiya, Atsushi Maruyama, Saori Odagiri, Fumiaki Mori, Ken Itoh, Akiyoshi Kakita, Hitoshi Takahashi and Koichi Wakabayashi
Organizer
15th International Congress of Immunology,
Place of Presentation
Milan, Italy
Related Report
-
-
-
-
-
-
-
-