• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Regulatory mechanism of p62 gene expression

Research Project

Project/Area Number 23500434
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

MATSUMOTO Gen  独立行政法人理化学研究所, 脳科学総合研究センター, 客員研究員 (50415303)

Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsタンパク質分解 / 選択的オートファジー / p62 / SQSTM1 / 薬剤スクリーニング / レポーター / レポーターアッセイ / リン酸化 / 薬剤ライブラリー
Outline of Final Research Achievements

p62/Sqstm1 is a key molecule for the selective autophagy. To investigate how to induce p62 in cells, we performed a drug screening that activated or inactivated p62 promoter. For this aim, we cloned human 1.8 kbp of p62 promoter region and inserted GFP gene under the predicted translation starting points. Using this reporter constructs, we identified five p62-inducer candidates. Two of them were obviously able to reduce polyglutamine aggregation in cells, but the S403-phospholylated p62 were not accumulated. On the other hand, one of the five candidates enhanced the polyglutamine aggregate formation and the accumulation of S403-phospho-p62. This may be possible that the drug may interfere the constitutive autophagy. Our results suggests that p62 promoter can be modulated artificially and the appropriate induction of p62 may be beneficial to induce selective autophagy.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2015 2014 2013 2012 2011

All Journal Article (10 results) (of which Peer Reviewed: 6 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Depletion of p62 reduces nuclear inclusions and paradoxically ameliorates disease phenotypes in Huntington's model mice.2015

    • Author(s)
      4)Kurosawa M, Matsumoto G, Kino Y, Okuno M, Kurosawa-Yamada M, Washizu C, Taniguchi H, Nakaso K, Yanagawa T, Warabi E, Shimogori T, Sakurai T, Hattori N, Nukina N.
    • Journal Title

      Hum Mol Genet.

      Volume: 24 Issue: 4 Pages: 1092-105

    • DOI

      10.1093/hmg/ddu522

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Dysregulation of the Proteasome Enhances the Toxicity of ALS-linked Mutant SOD12014

    • Author(s)
      Kitamura, A., Inada, N., Kubota, H., Matsumoto, G., Kinjo, M., Morimoto, R.I., Nagata, K
    • Journal Title

      Genes to Cells

      Volume: 3 Issue: 3 Pages: 209-224

    • DOI

      10.1111/gtc.12125

    • Related Report
      2014 Annual Research Report 2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phosphorylation of mitochondrial polyubiquitin by PINK1 promotes Parkin mitochondrial tethering.2014

    • Author(s)
      Shiba-Fukushima K, Arano T, Matsumoto G, Inoshita T, Yoshida S, Ishihama Y, Ryu KY, Nukina N, Hattori N, Imai Y.
    • Journal Title

      PLoS Genet.

      Volume: 10 Issue: 12 Pages: 1004861-1004861

    • DOI

      10.1371/journal.pgen.1004861

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] マイトファジーの認識機構ー選択的オートファジーの一形態としてー2014

    • Author(s)
      松本 弦
    • Journal Title

      医学のあゆみ

      Volume: 250 Pages: 489-494

    • Related Report
      2014 Annual Research Report
  • [Journal Article] NF-Y inactivation causes atypical neurodegeneration characterized by ubiquitin and p62 accumulation and endoplasmic reticulum disorganization2014

    • Author(s)
      Yamanaka, T., Tosaki, A., Kurosawa, M., Matsumoto, G., Koike, M., Uchiyama, Y., Maity, S.N., Shimogori, T., Hattori, N. & Nukina, N.
    • Journal Title

      Nat. Commun.

      Volume: 5 Issue: 1 Pages: 3354-3354

    • DOI

      10.1038/ncomms4354

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] p62のリン酸化とオートファジー2013

    • Author(s)
      松本 弦、貫名信行
    • Journal Title

      Annual Review 2013 神経, 編集:鈴木則宏、祖父江元、荒木信夫、宇川義一、川原信隆

      Volume: 1 Pages: 29-36

    • Related Report
      2013 Research-status Report
  • [Journal Article] ROCK-phosphorylated vimentin modifies mutant huntingtin aggregation via sequestration of IRBIT2012

    • Author(s)
      Bauer PO
    • Journal Title

      Molecular Neurodegeneration

      Volume: 7(1) Issue: 1 Pages: 43-43

    • DOI

      10.1186/1750-1326-7-43

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 選択的オートファジーによるユビキチン化タンパク質の処理機構2012

    • Author(s)
      松本 弦、貫名信行
    • Journal Title

      生体の科学 細胞の分子構造と機能ー核以外の細胞小器官

      Volume: 63 Pages: 490-491

    • Related Report
      2012 Research-status Report
  • [Journal Article] p62のリン酸化と選択的オートファジー2012

    • Author(s)
      松本 弦、貫名信行
    • Journal Title

      BIO Clinica 「神経筋疾患の分子標的治療開発」の各論

      Volume: 27 Pages: 18-22

    • Related Report
      2012 Research-status Report
  • [Journal Article] Serine 403 phosphorylation of p62/SQSTM1 regulates selective autophagic clearance of ubiquitinated proteins2011

    • Author(s)
      Matsumoto, G., et al
    • Journal Title

      Mol.Cell

      Volume: 44 Issue: 2 Pages: 279-289

    • DOI

      10.1016/j.molcel.2011.07.039

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] TBK1 controls PINK1/Parkin-dependent mitophagy through p62/SQSTM1 phosphorylation2014

    • Author(s)
      Gen Matsumoto
    • Organizer
      第37回日本神経科学会大会
    • Place of Presentation
      神奈川県横浜市 パシフィコ横浜
    • Year and Date
      2014-09-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] p62/SQSTM1リン酸化によるPINK1/Parkin依存性マイトファジーの制御機構2014

    • Author(s)
      松本 弦
    • Organizer
      第66回日本細胞生物学会大会
    • Place of Presentation
      奈良県奈良市 奈良県新公会堂
    • Year and Date
      2014-06-12
    • Related Report
      2014 Annual Research Report
  • [Presentation] p62タンパク質のリン酸化による選択的オートファジーの制御2013

    • Author(s)
      松本 弦
    • Organizer
      第65回 細胞生物学会「プロテオスタシス:細胞内タンパク質の恒常性」
    • Place of Presentation
      名古屋市
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] p62のリン酸化による選択的オートファジーの分子機構2013

    • Author(s)
      松本 弦
    • Organizer
      第7回オートファジー研究会 兼 新学術領域「オートファジー」第1回班会議
    • Place of Presentation
      静岡県掛川市
    • Related Report
      2013 Research-status Report
  • [Presentation] Phosphorylation of p62/SQSTM1 regulates selective autophagic clearance of ubiquitinated protein2011

    • Author(s)
      Matsumoto, G., Wada, K., Okuno, M., Kurosawa, M., and Nukina, N.
    • Organizer
      The 2011 Cold Spring Harbor Laboratory meeting on "The Ubiquitin Family"
    • Place of Presentation
      Cold Spring Harbor, NY, USA
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi