The role of prostaglandins in myelination and axonal protection by oligodendrocytes

• Project/Area Number: 23500436
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Kagawa University (2013); Institute for Developmental Research, Aichi Human Service Center (2011-2012)
• Principal Investigator: CHIBA Yoichi 香川大学, 医学部, 講師 (30372113)
• Co-Investigator(Renkei-kenkyūsha): WATANABE Kikudo 甲子園大学, 栄養学部, 教授 (90211672) ; MIURA Koshiro 川崎医科大学, 医学部, 講師 (30284243) ; SHIMADA Atsuyoshi 愛知県心身障害者コロニー発達障害研究所, 病理学部, 室長 (50311444)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: オリゴデンドロサイト / プロスタグランジンF2alpha / プロスタグランジン / 脱髄疾患 / 髄鞘 / 軸索保護 / ニューロン・グリア相互作用

Research Abstract

Physiological roles of prostaglandin (PG) F2alpha in central nervous system have not been elucidated. We studied the function of PG2alpha in oligodendrocyte-lineage cells, since our previous study revealed oligodendroglial localization of prostamide/PGF synthase, a major PGF2alpha-producing enzyme in mouse central nervous system. We confirmed expression of prostamide/PGF synthase, production of PGF2alpha, and an increase in intracellular calcium concentration in response to exogenous PGF2alpha, in oligodendrocytes. PGF2alpha seemed to be essential for survival of oligodendrocyte-lineage cells in their early development, since oigodendrocyte precursors from prostamide/PGF synthase-knockout mice were not able to survive without PGF2alpha supplementation. In contrast, oligodendrocyte-lineage cells from knockout mice showed normal maturation in response to differentiation stimuli.

Research Products

• [Journal Article] Niemann-Pick disease type Cl predominantly involving the front otemporal region, with cortical and brainstem Lewy bodies : An autopsy case. (2014)
  • Author(s): Chiba Y., Komori H., Takei S., Hasegawa-Ishii S., Kawamura N., Adachi K., Nanba E., Hosokawa M., Enokido Y., Kouchi Z., Yoshida F., Shimada A.
  • Journal Title: Neuropathology : Volume: 34(1) Issue: 1 Pages: 49-57
  • DOI: 10.1111/neup.12047
  • Peer Reviewed
• [Journal Article] Immunoreactivity of glucose transporter 5 is located in epithelial cells of the choroid plexus and ependymal cells. (2014)
  • Author(s): Ueno M, Nishi N, Nakagawa T, Chiba Y, Tsukamoto I, Kusaka T, Miki T, Sakamoto H, Yamaguchi F, Tokuda M.
  • Journal Title: Neuroscience Volume: 260 Pages: 149-157
  • DOI: 10.1016/j.neuroscience.2013.12.006
  • Peer Reviewed / Open Access
• [Journal Article] Excitotoxicity-induced immediate surge in hippocampal prostanoid production has latent effects that promote chronic progressive neuronal death. (2013)
  • Author(s): Yoshikawa K., Kita Y., Furukawa A., Kawamura N., Hasegawa-Ishii S., Chiba Y., Takei S., Maruyama K., Shimizu T., Shimada A.
  • Journal Title: Prostaglandins Leukot Essent Fatty Acids. Volume: 88(5) Issue: 5 Pages: 373-381
  • DOI: 10.1016/j.plefa.2013.02.007
  • Peer Reviewed
• [Journal Article] Spontaneous occurrence of photoageing-like phenotypes in the dorsal skin of old SAMP1 mice, an oxidative stress model. (2013)
  • Author(s): Sakura M.
  • Journal Title: Exp Dermatol. Volume: 22 Issue: 1 Pages: 62-64
  • DOI: 10.1111/exd.12059
  • Peer Reviewed
• [Journal Article] Immunohistochemical demonstration of increased prostaglandin F2α levels in the rat hippocampus following kainic acid-induced seizures (2012)
  • Author(s): Takei S, Hasegawa-Ishii S, Uekawa A, Chiba Y, Umegaki H, Hosokawa M, Woodward DF, Watanabe K, Shimada A
  • Journal Title: Neuroscience Volume: 218 Pages: 295-304
  • Peer Reviewed
• [Journal Article] Immunohistochemical localization of aggresomal proteins in glial cytoplasmic inclusions in multiple system atrophy. (2012)
  • Author(s): Chiba Y., Takei S., Kawamura N., Kawaguchi Y., Sasaki K., Hasegawa-Ishii S., Furukawa A., Hosokawa M., Shimada A.
  • Journal Title: Neuropathol. Appl. Neurobiol. Volume: 38(6) Issue: 6 Pages: 559-71
  • DOI: 10.1111/j.1365-2990.2011.01229.x
  • Peer Reviewed
• [Journal Article] Immunohistochemical demonstration of increased prostaglandin F2alpha levels in the rat hippocampus following kainic acid-induced seizures (2012)
  • Author(s): Takei, S., Hasegawa-Ishii, S., Uekawa, A., Chiba, Y., Umegaki, H., Hosokawa, M., Woodward, D.F., Watanabe, K., and Shimada, A.
  • Journal Title: Neuroscience Volume: 218 Pages: 295-304
  • DOI: 10.1016/j.neuroscience.2012.05.013
  • Peer Reviewed
• [Journal Article] Selective localization of bone marrow-derived ramified cells in the brain adjacent to the attachments of choroid plexus. (2012)
  • Author(s): Hasegawa-Ishii S, Shimada A, Inaba M, Li M, Shi M, Kawamura N, Takei S, Chiba Y, Hosokawa M, Ikehara S.
  • Journal Title: Brain Behav Immun. Volume: 29 Pages: 82-97
  • DOI: 10.1016/j.bbi.2012.12.010
  • Peer Reviewed
• [Journal Article] アミロイドのコンゴーレッド染色標本観察における封入剤の影響 (2012)
  • Author(s): 河村則子、千葉陽一、佐倉正明、細川正則
  • Journal Title: 実験病理組織技術研究会誌 Volume: 21 Pages: 65-73
  • Peer Reviewed
• [Journal Article] Proteomic identification of hippocampal proteins vulnerable to oxidative stress in excitotoxin-induced acute neuronal injury (2011)
  • Author(s): A. Furukawa, Y. Kawamoto, Y. Chiba, S.Takei, S. Hasegawa-Ishii, N. Kawamura, K. Yoshikawa, M. Hosokawa, S. Oikawa, M. Kato, A. Shimada
  • Journal Title: Neurobiology of disease Volume: 43 Issue: 3 Pages: 706-714
  • DOI: 10.1016/j.nbd.2011.05.024
  • Peer Reviewed
• [Presentation] 脳と免疫系の接点としての脈絡叢間質および付着部における細胞動態とサイトカイン発現 (2013)
  • Author(s): 石井さなえ, 島田厚良, 稲葉宗夫, 李銘, 石明, 河村則子, 武井史郎, 千葉陽一, 細川昌則, 池原進
  • Organizer: 第54回日本神経病理学会
  • Place of Presentation: 東京都
  • Year and Date: 2013-04-25
• [Presentation] Immunohistochemical demonstration of enhanced prostaglandin F2a production following kainic acid-induced seizures in rat hippocampus (2012)
  • Author(s): Takei S, Hasegawa-Ishii S, Uekawa A, Chiba Y, Umegaki H, Hosokawa M, Woodward DF, Watanabe K, Shimada A
  • Organizer: 14th International Congress of Histochemistry and Cytochemistry
  • Place of Presentation: Kyoto
  • Year and Date: 2012-08-29
• [Presentation] 前頭側頭葉優位の脳萎縮を呈した若年型Niemann-Pick病C 型の一剖検例 (2012)
  • Author(s): 千葉陽一, 小森拓, 吉田太, 足立香織, 難波栄二, 武井史郎, 石井さなえ, 河内全, 榎戸靖, 細川昌則, 島田厚良
  • Organizer: 第53回日本神経病理学会
  • Place of Presentation: 新潟市
  • Year and Date: 2012-06-30
• [Presentation] 老化促進モデルマウスにみられる骨髄由来細胞の脳実質へのリクルートの亢進 (2012)
  • Author(s): 島田厚良, 石井さなえ, 稲葉宗夫, 李銘, 石明, 河村則子, 武井史郎, 千葉陽一, 榎戸靖, 河内全, 細川昌則, 池原進
  • Organizer: 第53回日本神経病理学会
  • Place of Presentation: 新潟市
  • Year and Date: 2012-06-30
• [Presentation] 前頭側頭葉優位の脳萎縮を呈した若年型Niemann-Pick病C型の一剖検例 (2012)
  • Author(s): 千葉陽一、小森拓、吉田太、足立香織、難波栄二、武井史郎、石井さなえ、河内全、榎戸靖、細川昌則、島田厚良
  • Organizer: 第５３回日本神経病理学会
  • Place of Presentation: 朱鷺メッセ（新潟市）
• [Presentation] Immunohistochemical demonstration of enhanced prostaglandin F2α production following kainic acid-induced seizures in rat hippocampus (2011)
  • Author(s): Takei S, Hasegawa-Ishii S, Uekawa A, Furukawa A, Chiba Y, Kawamura N, Hosokawa M, Woodward DF, Watanabe K, Shimada A
  • Organizer: 12th International Conference on Bioactive Lipids in Cancer, Inflammation, and Related Diseases
  • Place of Presentation: Seattle, USA
  • Year and Date: 2011-09-19
• [Presentation] カイニン酸誘導てんかん発作に伴う海馬プロスタグランジンF_<2α>産生更新の免疫組織学的解析 (2011)
  • Author(s): 武井史郎, 石井さなえ, 上川篤志, 千葉陽一, 河村則子, Woodward DF, 渡部紀久子, 島田厚良
  • Organizer: 第52回日本神経病理学会
  • Place of Presentation: 京都市
  • Year and Date: 2011-06-04
• [Presentation] 内側側頭葉てんかん患者の海馬における酸化損傷タンパク質の解析 (2011)
  • Author(s): 古川絢子、柿田明美、千葉陽一 他
  • Organizer: 第52回日本神経病理学会
  • Place of Presentation: 京都
• [Presentation] 老化促進モデルマウスにみられるサイトカインを介した神経保護的グリア応答機能の低下 (2011)
  • Author(s): 石井さなえ、武井史郎、稲葉宗夫、千葉陽一他
  • Organizer: 第52回日本神経病理学会
  • Place of Presentation: 京都
• [Presentation] カイニン酸誘導てんかん発作に伴う海馬プロスタグランジンF2a産生亢進の免疫組織学的解析 (2011)
  • Author(s): 武井史郎、石井さなえ、上川篤志、古川絢子、千葉陽一他
  • Organizer: 第52回日本神経病理学会
  • Place of Presentation: 京都
• [Presentation] 学習の遅れと歩行障害で発症し、多彩な神経症状が進行した男性の剖検例 (2011)
  • Author(s): 千葉陽一、武井史郎、石井さなえ、細川昌則、島田厚良、小森拓
  • Organizer: 第３回日本神経病理学会東海・北陸地方会
  • Place of Presentation: 長久手
• [Presentation] アストロサイト系譜細胞の代謝異常によって生じる脳発達障害メカニズムの解析 (2011)
  • Author(s): 榎戸靖、長谷川弘、河内全、千葉陽一、島田厚良、細川昌則
  • Organizer: 第８４回日本生化学会
  • Place of Presentation: 京都