Functional analysis of clusterd protocadherin during neural circuit formation
| Project/Area Number |
23500447
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | プロトカドヘリン / 神経回路形成 / 接着分子 / 遺伝子改変マウス / protocadherin / gene targeting / iPS cells / 分子的多様性 |
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| Research Abstract |
The clustered protocadherin (cPcdh) genes encode diverse transmembrane proteins, which divide into three Pcdh-a, -b and -g clusters. Distinct sets of Pcdh genes are differentially expressed in individual neurons. The diversity of the cPcdh genes may contribute to selective interactions between neurons and be required for neural circuit formation.
In this study, to elucidate the physiological significance of cPcdh diversity, we analyzed adhesive property of cPcdhs by cell aggregation assay, and produced mice with a conditional knockout of cPcdh gene in telencephalon. Cell aggregation assay using K562 cells, which express cPcdhs revealed that Pcdh-alpha and Pcdh-beta have homophilic adhesive activity.The cPcdh conditional knockout mice were viable, but display abnormalities in the structure of the cerebral cortex.The detailed phenotypic analysis of these mutant mice are in progress.
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Developmental epigenetic modification regulates stochastic expression of clustered protocadherin genes, generating single neuron diversity.2014
Author(s)
Toyoda S, Kawaguchi M, Kobayashi T, Tarusawa E, Toyama T, Okano M, Oda M, Nakauchi H, Yoshimura Y, Sanbo M, Hirabayashi M, Hirayama T, Hirabayashi T, Yagi T.
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Journal Title
Neuron
Volume: 82
Issue: 1
Pages: 94-108
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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