| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
The clustered protocadherin (cPcdh) genes encode diverse transmembrane proteins, which divide into three Pcdh-a, -b and -g clusters. Distinct sets of Pcdh genes are differentially expressed in individual neurons. The diversity of the cPcdh genes may contribute to selective interactions between neurons and be required for neural circuit formation.
In this study, to elucidate the physiological significance of cPcdh diversity, we analyzed adhesive property of cPcdhs by cell aggregation assay, and produced mice with a conditional knockout of cPcdh gene in telencephalon. Cell aggregation assay using K562 cells, which express cPcdhs revealed that Pcdh-alpha and Pcdh-beta have homophilic adhesive activity.The cPcdh conditional knockout mice were viable, but display abnormalities in the structure of the cerebral cortex.The detailed phenotypic analysis of these mutant mice are in progress.
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