Functional maturation of the control of the mammalian spinal locomotor network

• Project/Area Number: 23500460
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: University of Tsukuba
• Principal Investigator: NISHIMARU Hiroshi 筑波大学, 医学医療系, 准教授 (20302408)
• Co-Investigator(Kenkyū-buntansha): KOGANEZAWA Tadachika 筑波大学, 医学医療系, 助教 (80431691)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 神経回路 / 歩行 / 運動 / マウス / 脊髄 / 神経科学 / 生理学 / ニューロン / シナプス

Research Abstract

In this study we used genetically-modified mice that show abnormal gait to investigate the function and maturation of the locomotor network in the spinal cord especially how it is controlled by the supraspinal structures. Our findings suggest that the corticospinal tract plays little role in the development of the spinal locomotor network and that a population of EphA4-positive spinal excitatory neurons play a significant role in the normal development of the mammalian locomotor circuit.

Research Products

• [Journal Article] Spinal glutamatergic neurons defined by EphA4 signaling are essential components of normal locomotor circuits (2014)
  • Author(s): Borgius L, Nishimaru H, Caldeira V, Kunugise Y, Low P, Reig R, Itohara S, Iwasato T, Kiehn O
  • Journal Title: Journal of Neuroscience Volume: 34 Pages: 3841-3853
  • Peer Reviewed
• [Journal Article] EphA4-dependent axon retraction and midline localization of Ephrin-B3 are disrupted in the spinal cord of mice lacking mDia1 and mDia3 in combination (2013)
  • Author(s): Toyoda Y, Shinohara R, Thumkeo D, Kamijo H, Nishimaru H, Hioki H, Kaneko T, Ishizaki T, Furuyashiki T, Narumiya S
  • Journal Title: Genes to Cells Volume: 18 Pages: 873-885
  • Peer Reviewed
• [Journal Article] EphA4-dependent axon retraction and midline localization of Ephrin-B3 are disrupted in the spinal cord of mice lacking mDial and mDia3 in combination (2013)
  • Author(s): Yosuke Toyoda, Ryota Shinohara, Dean Thumkeo, Hiroshi Kamijo, Hiroshi Nishimaru, Hiroyuki Hioki, Takeshi Kaneko, Toshimasa Ishizaki, Tomoyuki Fnruyashiki and Shuh Narumiva
  • Journal Title: Genes to Cells Volume: 18 Issue: 10 Pages: 873-885
  • DOI: 10.1111/gtc.12081
  • Peer Reviewed
• [Journal Article] 哺乳類脊髄神経回路網の作動機序と機能発達 (2012)
  • Author(s): 西丸広史
  • Journal Title: ブレインサイエンス・レビュー(クバプロ) Pages: 83-106
• [Journal Article] 歩行運動発現における脊髄抑制性ニューロンの役割 (2012)
  • Author(s): 西丸広史
  • Journal Title: Clin Neurosci Volume: 30 Pages: 1371-1374
• [Journal Article] 歩行運動発現における脊髄抑制性ニューロンの役割 (2012)
  • Author(s): 西丸広史
  • Journal Title: Clinical Neuroscience Volume: 30 Pages: 1371-1374
• [Presentation] 哺乳類歩行運動神経回路における興奮性ニューロンの役割 : キメリンノックアウトマウスの機能解析 からわかること (2014)
  • Author(s): 西丸広史
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学, 鹿児島
  • Year and Date: 2014-03-17
• [Presentation] 哺乳類歩行運動神経回路における興奮性ニューロンの役割:キメリンノックアウトマウスの機能解析からわかること (2014)
  • Author(s): 西丸広史
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学（鹿児島市）
  • Invited
• [Presentation] サル頸髄前角におけるレンショウ細胞の分布 : 免疫組織学的解析Rostrocaudal distribution of calbindin D28k positive cells in the ventral horn of the cervical spinal cord in monkeys (2013)
  • Author(s): 西丸広史, 他
  • Organizer: 36回日本神経科学会大会
  • Place of Presentation: 京都国際会議場, 京都
  • Year and Date: 2013-06-20
• [Presentation] Abnormal output pattern of the spinal locomotor circuit of the VGLUT2-positive-neuron-specific α -chimerin knockout mouse (2012)
  • Author(s): Nishimaru H, 他
  • Organizer: The Society for Neuroscience 42nd Annual Meeting
  • Place of Presentation: New Orleans USA
  • Year and Date: 2012-10-16
• [Presentation] シンポジウム「運動の出力パターンを形成する脳幹・脊髄介在ニューロンの機能発達」マウス脊髄運動中枢における抑制性介在ニューロンの機能と発達分化 (2012)
  • Author(s): 西丸広史
  • Organizer: 第35回日本神経科学会大会
  • Place of Presentation: 名古屋国際会議場, 名古屋
  • Year and Date: 2012-09-18
• [Presentation] 哺乳類の歩行中枢パターン発生回路の機能的構成 (2012)
  • Author(s): 西丸広史
  • Organizer: 第6回 Motor Contol 研究会シンポジウム「CPG 研究の現状と展望:これまでにわかっていること・わかっていないこと」
  • Place of Presentation: 岡崎コンファレンスセンター, 岡崎市
  • Year and Date: 2012-06-22
• [Presentation] 興奮性ニューロン特異的α-キメリンノックアウトマウス脊髄における歩行中枢の機能解析 Altered left-right activation pattern in the spinal locomotor circuit of the excitatory neuron-specific alpha-chimerin knockout mouse (2012)
  • Author(s): 椚瀬佑果, 他
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 長野県松本文化会館, 松本市
  • Year and Date: 2012-03-31
• [Presentation] CPG研究の現状と展望： これまでにわかっていること・わかっていないこと」 (2012)
  • Author(s): 西丸広史
  • Organizer: 第6回Motor Contol 研究会
  • Place of Presentation: 岡崎市
  • Invited
• [Presentation] マウス脊髄運動中枢における抑制性介在ニューロンの機能と発達分化 (2012)
  • Author(s): 西丸広史
  • Organizer: 第35回日本神経科学会大会
  • Place of Presentation: 名古屋市
  • Invited
• [Presentation] Abnormal output pattern of the spinal locomotor circuit of the VGLUT2-positive-neuron-specific alpha-chimerin knockout mouse (2012)
  • Author(s): Nishimaru H, Borgius L, Iwasato T, Kunugise Y, Itohara S, Kiehn O
  • Organizer: The Society for Neuroscience 41st Annual Meeting
  • Place of Presentation: New Orleans, USA
• [Presentation] Unravelling the neuronal mechanism of the spinal locomotor network -what we learn from hopping mice- (2012)
  • Author(s): Nishimaru H
  • Organizer: International Symposium on Organization and Function of the Nervous System
  • Place of Presentation: 東京
  • Invited
• [Presentation] 興奮性ニューロン特異的aキメリンノックアウトマウス脊髄における歩行中枢の機能解析 (2012)
  • Author(s): 椚瀬 佑果、L. Borgius、岩里 琢治、糸原 重美、O. Kiehn、西丸 広史
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本市
• [Presentation] The excitatory EphA4-related population is involved in coordinating the mammalian locomotor CPG (2011)
  • Author(s): Borgius L, 他
  • Organizer: The Society for Neuroscience 41st Annual Meeting
  • Place of Presentation: Washington D.C. USA
  • Year and Date: 2011-11-14
• [Presentation] The excitatory EphA4-related population is involved in coordinating the mammalian locomotor CPG (2011)
  • Author(s): L. Borgius, H. Nishimaru, G. Margaryan, B. Zheng, T. Iwasato, O. Kiehn
  • Organizer: Society for Neuroscience, Neuroscience 2011
  • Place of Presentation: ワシントンDC、アメリカ合衆国