| Project/Area Number |
23500475
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
WATANABE MASARU 首都大学東京, 人間健康科学研究科, 教授 (60191798)
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| Co-Investigator(Renkei-kenkyūsha) |
ISHIDA YUKISATO 文京学院大学, 保健医療学部, 教授 (50092135)
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| Project Period (FY) |
2011-04-28 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 平滑筋 / 収縮フィラメント / リモデリング / アクチン / ミオシン / X線回折 / フィラメント / ATP / Ｘ線回折 / NMR / 収縮 / 筋収縮フィラメント / 構造 / 細胞 / 力学応答 |
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| Outline of Final Research Achievements |
To explore the mechanisms of regulation of actin-myosin interaction induced by remodeling of contractile filaments in living smooth muscle, we examined X-ray diffraction studies and also force measurements in skinned (cell membrane permeabilized) smooth muscle. In skinned taenia cecum in the resting state, myosin-remodeling induced diffuse equatorial peak at 11.4 nm originated from lattice-like arrangement of the thin filaments, indicating that functional changes in smooth muscle myosin molecules regulate the organization of thick-filaments, resulting in the alteration of thin-filaments structure and/or organization even in the absence of cross-bridge formation.
Under the same experimental conditions, we found that myosin remodeling changed chemo-mechanical coupling of skinned taenia cecum, because such a stimuli accelerated relaxation processes by interference of latch-bridge formation.
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