| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Calpain-3 (CAPN3) is predominantly expressed in skeletal muscle and is Ca2+-requiring intracellular cysteine protease. In this study, we made sensor-proteins to detect CAPN3 protease activity using a fluorescence resonance energy transfer technique and examined whether CAPN3 protease activity was controlled by phosphorylation. Unfortunately, it was very tough to detect protease activity of CAPN3 in living muscle cells although sensor-proteins were expressed well and were cleaved by CAPN3 in vitro experiments. On the other hand, we showed that rapid and exhaustive autolysis of CAPN3 was slightly attenuated by the defect of phosphorylation of Ser residue at 629 of CAPN3.
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