Analysis of physiological functions of novel endothelial-enriched genes

• Project/Area Number: 23500488
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Shiga University of Medical Science (2013); University of Tsukuba (2011-2012)
• Principal Investigator: EMA Masatsugu 滋賀医科大学, 動物生命科学研究センター, 教授 (60359578)
• Co-Investigator(Kenkyū-buntansha): TAKAHASHI Satoru 筑波大学, 医学医療系, 教授 (50271896)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 血管新生 / モデルマウス / 血管内皮細胞 / 網膜 / がん / ライブイメージング / 蛍光タンパク質

Research Abstract

Blood vessels are therapeutic targets for cancer, ischemic diseases and diabetes-related diseases. Therefore, understanding the process of angiogenesis is important for not only intellectual but potentially development of medicine for such diseases. A novel Rho-related gene, RhoJ was investigated for its physiological function by knocking out. The RhoJ KO exhibited retarded angiogenesis in the retina. Since the retina showed empty sleeves, regression seems to be one of causes for the retarded angiogenesis.
Flk1-GFP :: Flt1-tdsRed BAC Tg mice were created to monitor the endogenous expression of Flk1 and Flt1. Based on the expression of Flk1-GFP and Flt1-tdsRed, Flk1 and Flt1 are expressed in micro capillaries and mid/major endothelial cells, respectively.

Research Products

• [Journal Article] C1galt1-deficient mice exhibit thrombocytopenia due to abnormal terminal differentiation of megakaryocytes (2013)
  • Author(s): Kudo T, Sato T, Hagiwara K, Kozuma Y, Yamaguchi T, Ikehara Y, Hamada M, Matsumoto K, Ema M, Murata S, Ohkohchi N, Narimatsu H, Takahashi S
  • Journal Title: Blood Volume: 122 Pages: 1649-1657
  • Peer Reviewed
• [Journal Article] Klf9 is necessary and sufficient for Purkinje cell survival in organotypic culture (2013)
  • Author(s): Lebrun C., Avci HX., Wehrlé R., Doulazmi M., Jaudon F., Morel MP., Rivals I., Ema M., Schmidt S., Sotelo C., Vodjdani G., Dusart I
  • Journal Title: Mol Cell Neurosci Volume: 54 Pages: 9-21
  • Peer Reviewed
• [Journal Article] Bioluminescence imaging of β cells and intrahepatic insulin gene activity under normal and pathological conditions (2013)
  • Author(s): Katsumata T, Oishi H, Sekiguchi Y, Nagasaki H, Daassi D, Tai PH, Ema M, Kudo T, Takahashi S
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Clgalt1-deficient mice exhibit thrombocytopenia due to abnormal terminal differentiation of megakaryocytes (2013)
  • Author(s): Kudo T, et al. (13人中8番目)
  • Journal Title: Blood Volume: 122 Issue: 9 Pages: 1649-1657
  • DOI: 10.1182/blood-2012-12-471102
  • Peer Reviewed
• [Journal Article] Delayed cutaneous wound healing in Fam129b/Minerva-deficient mice (2012)
  • Author(s): Oishi H, Itoh S, Matsumoto K, Ishitobi H, Suzuki R, Ema M, Kojima T, Uchida K, Kato M, Miyata T, Takahashi S
  • Journal Title: J. Biochem Volume: 152 Pages: 549-555
  • NAID: 10031166173
  • Peer Reviewed
• [Journal Article] Flt1 and Flk1 mediate regulation of intraocular pressure and their double heterozygosity causes the buphthalmia in mice (2012)
  • Author(s): Sano K., Katsuta O., Shirae S., Kubota Y., Ema M., Suda T., Nakamura M., Hirashima M
  • Journal Title: Biochem Biophys Res Commun Volume: 420 Pages: 422-427
  • Peer Reviewed
• [Journal Article] Thyroid hormone triggers the developmental loss of axonal regenerative capacity via thyroid hormone receptor α1 and kruppel-like factor 9 in Purkinje cells (2012)
  • Author(s): Avci HX., Lebrun C., Wehrlé R., Doulazmi M., Chatonnet F., Morel MP., Ema M., Vodjdani G., Sotelo C., Flamant F., Dusart I
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Pages: 14206-14211
  • Peer Reviewed
• [Journal Article] Study of normal and pathological blood vessel morphogenesis in Flt1-tdsRed BAC Tg mice (2012)
  • Author(s): Matsumoto K., Azami T., Otsu A., Takase H., Ishitobi H., Tanaka J., Miwa Y., Takahashi S., Ema M
  • Journal Title: Genesis Volume: 50 Pages: 561-571
  • Peer Reviewed
• [Journal Article] Study of normal and pathological blood vessel morphogenesis in Flt1-tdsRed BAC Tg mice (2012)
  • Author(s): Matsumoto K, Azami T, Otsu A, Takase H, Ishitobi H, Tanaka J, Miwa Y, Takahashi S, Ema M
  • Journal Title: Genesis Volume: 50 Issue: 7 Pages: 561-571
  • DOI: 10.1002/dvg.22031
  • Peer Reviewed
• [Journal Article] Genome-wide identification of endothelial cell-enriched genes in the mouse embryo (2012)
  • Author(s): Takase H, Matsumoto H, Yamadera R, Kubota Y, Otsu A, Suzuki R, Ishitobi H, Mochizuki H, Kojima T, Takano S, Uchida K, Takahashi S, Ema M
  • Journal Title: Blood Volume: 120 Issue: 4 Pages: 914-923
  • DOI: 10.1182/blood-2011-12-398156
  • Peer Reviewed
• [Journal Article] Genome-wide identification of endothelial cell-enriched genes in the mouse embryo
  • Author(s): Takase H., Matsumoto K., Yamadera R., Kubota Y., Otsu A., Suzuki R., Ishitobi H., Mochizuki H., Kojima T., Takano S., Uchida K., Takahashi S., Ema M
  • Journal Title: Blood Volume: 120 Pages: 914-923
  • Peer Reviewed
• [Presentation] Origin of cardiovascular cells and heart development controlled by VEGF-Flk1 signaling (2014)
  • Author(s): Masatsugu Ema, Ayaka Otsu, Takuya Azami, Masanori Hirashima , Kazuko Koshiba-Takeuchi , Jun Takeuchi , Masabumi Shibuya, Janet Rossant, Satoru Takahashi, Tomomi Nishie
  • Organizer: 第91回生理学会大会, シンポジウム講演
  • Place of Presentation: 鹿児島
• [Presentation] Origin of cardiovascular cells and heart development controlled by VEGF-Flk1 signaling (2014)
  • Author(s): 依馬 正次
  • Organizer: 日本生理学会
  • Place of Presentation: 鹿児島
  • Invited
• [Presentation] VEGF-Flk1 による心臓発生のメカニズム (2013)
  • Author(s): 西江 友美, 大津 彩香, 平島 正則, 小柴 和子, 竹内 純, 渋谷 正史, Janet Rossant, 高橋 智, 依 馬 正次 次
  • Organizer: 第36回分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 心血管系細胞の起源と血管による心臓発生制御 (2013)
  • Author(s): 依馬 正次
  • Organizer: 第36回分子生物学会年会(ワークショップ)オーガナイザー
  • Place of Presentation: 神戸
• [Presentation] 血管内皮細胞関連遺伝子の網羅的探索による血管形成機序の解明 (2013)
  • Author(s): 依馬 正次
  • Organizer: 第21回日本血管生物医学会学術集会
  • Place of Presentation: 大阪
• [Presentation] 心血管系細胞の起源と血管による心臓発生制御 (2013)
  • Author(s): 依馬 正次
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Invited
• [Presentation] Genome-wide identification of angiogenesis- related genes in the mouse embryo (2012)
  • Author(s): Masatsugu Ema
  • Organizer: 第21回日本血管生物医学会学術集会
  • Place of Presentation: 徳島
• [Presentation] 血管内皮細胞の発生・分化の分子機構 (2012)
  • Author(s): 依馬正次
  • Organizer: 日本解剖学会総会(招待講演)
  • Place of Presentation: 甲府
• [Presentation] 新規血管内皮細胞特異的遺伝子群の網羅的探索 (2011)
  • Author(s): 松本 健, 高瀬 春華, 山寺 里枝, 大津 彩香, 石飛 博之, 小島 崇宏, 内田 和彦, 高橋 智, 依馬 正次
  • Organizer: 第34回分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Genome-wide discovery of novel gene (2011)
  • Author(s): 依馬正次
  • Organizer: 日本血管生物医学会
  • Place of Presentation: 東京
• [Presentation] Genome-wide discovery of novel gene (2011)
  • Author(s): 依馬正次
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜
• [Book] 発生期における血管形成制御因子の網羅的探索 (2013)
  • Author(s): 依馬 正次, 吉原 雅大
  • Publisher: メディカルレビュー社