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Generation of transplantable and gene therapy model rats using IL2 receptor gamma KO rat derived from rat pluripotent stem cell

Research Project

Project/Area Number 23500490
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionThe University of Tokyo

Principal Investigator

HAMANAKA SANAE  東京大学, 医科学研究所, 特任研究員 (40511415)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsX-SCID / 移植 / ラットES細胞 / ラットiPS細胞 / IL2レセプターγ遺伝子
Research Abstract

We generated a model rat of the X-linked serious case combined immunodeficiency disease (X-SCID) by homologous recombination with IL2 receptor gammma gene using rat pluripotent stem cells. T cells, B cells and natural killer cells and the thymus were decreased in the X-SCID rat and it showed the serious case immune deficiency disorder.
It was suggested that the X-SCID rat was useful as a model of the cell transplantation of a neoplastic cell of human and mouse hematopoietic stem cells, even though the human hematopoietic stem cells were not able to reconstitution.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (11 results)

All 2013 2012 2011

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (1 results)

  • [Journal Article] Clonal analysis unveils self-renewing lineage-restricted progenitors generated directly from hematopoietic stem cells2013

    • Author(s)
      Yamamoto R, Morita Y, Ooehara J, Hamanaka S, Onodera M, Rudolph KL, Ema H, Nakauchi H
    • Journal Title

      Cell

      Volume: 154(5) Pages: 1112-26

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of transgenic mouse line expressing Kusabira Orange throughout body, including erythrocytes, by random segregation of provirus method2013

    • Author(s)
      Hamanaka S, Ooehara J, Morita Y, Ema H, Takahashi S, Miyawaki A, Otsu M, Yamaguchi T, Onodera M, Nakauchi H
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 435(4) Pages: 586-91

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of transgenic mouse line expressing Kusabira Orange throughout body, including erythrocytes, by random segregation of provirus method2013

    • Author(s)
      Hamanaka S, Ooehara J, Morita Y, Ema H, Takahashi S, Miyawaki A, Otsu M, Yamaguchi T, Onodera M, Nakauchi H
    • Journal Title

      Biochem Biophys Res Commun

      Volume: i435(4) Issue: 4 Pages: 586-91

    • DOI

      10.1016/j.bbrc.2013.05.017

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clonal analysis unveils self-renewing lineage-restricted progenitors generated directly from hematopoietic stem cells.2013

    • Author(s)
      Yamamoto R, Morita Y, Ooehara J, Hamanaka S, Onodera M, Rudolph KL, Ema H, Nakauchi H.
    • Journal Title

      Cell.

      Volume: 154 Issue: 5 Pages: 1112-26

    • DOI

      10.1016/j.cell.2013.08.007

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an all-in-one inducible lentiviral vector for gene specific analysis of reprogramming2012

    • Author(s)
      Yamaguchi T, Hamanaka S, Kamiya A, Okabe M, Kawarai M, Wakiyama Y, Umino A, Hayama T, Sato H, Lee YS, Kato-Itoh M, Masaki H, Kobayashi T, Yamazaki S, Nakauchi H
    • Journal Title

      PLoS One

      Volume: 7(7)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distinct B-cell lineage commitment distinguishes adult bone marrow hematopoietic stem cells2012

    • Author(s)
      Ghosn EE, Yamamoto R, Hamanaka S, Yang Y, Herzenberg LA, Nakauchi H, Herzenberg LA
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 109(14) Pages: 5394-8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distinct B-cell lineage commitment distinguishes adult bone marrow hematopoietic stem cells.2012

    • Author(s)
      Ghosn EE, Yamamoto R, Hamanaka S, Yang Y, Herzenberg LA, Nakauchi H, Herzenberg LA
    • Journal Title

      Proc Natl Acad Sci U S A.

      Volume: 3:109(4) Pages: 5394-5398

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of an all-in-one inducible lentiviral vector for gene specific analysis of reprogramming.2012

    • Author(s)
      Yamaguchi T, Hamanaka S, Kamiya A, Okabe M, Kawarai M, Wakiyama Y, Umino A, Hayama T, Sato H, Lee YS, Kato-Itoh M, Masaki H, Kobayashi T, Yamazaki S, Nakauchi H.
    • Journal Title

      PLoS One.

      Volume: 7(7)

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Generation of germline-competent rat induced pluripotent stem cells2011

    • Author(s)
      Hamanaka S, Yamaguchi T, Kobayashi T, Kato-Itoh M, Yamazaki S, Sato H, Umino A, Wakiyama Y, Arai M, Sanbo M, Hirabayashi M, Nakauchi H
    • Journal Title

      PLoS One

      Volume: 6(7)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of germline-competent rat induced pluripotent stem cells.2011

    • Author(s)
      Hamanaka S, Yamaguchi T, Kobayashi T, Kato-Itoh M, Yamazaki S, Sato H, Umino A, Wakiyama Y, Arai M, Sanbo M, Hirabayashi M, Nakauchi H.
    • Journal Title

      PLoS One

      Volume: 6(7)

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] GENERATION OF GERMLINE COMPITENT RAT INDUCED PLURIPOTENT STEM CELL2011

    • Author(s)
      Sanae Hamanaka
    • Organizer
      ISSCR 9th Annual Meeting
    • Place of Presentation
      Toronto, Ontario Canada
    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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