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Live-cell imaging to monitor X-chromosome reactivation and stem cell reasearch

Research Project

Project/Area Number 23500492
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KOBAYASHI Shin  東京医科歯科大学, 難治疾患研究所, 非常勤講師 (10397664)

Co-Investigator(Kenkyū-buntansha) OKABE Masaru  大阪大学, 微生物病研究所, 教授 (30089875)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords遺伝子組換え動物 / X染色体不活性化 / イメージング / ライブイメージング / 遺伝子組み替え / 遺伝子組み替え動物
Research Abstract

In stem cell research, reactivation of X chromosomes is recognized as an indicator of the pluripotent grand state of stem cells. However, there is no report of the monitoring method on this phenomenon. Therefore, we attempt to develop a novel method to monitor this phenomenon based on a live-imaging technique. This method would help us to understand the reprogramming process at the molecular level. The outcomes of this search will have considerable impact in medical care.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (32 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (22 results) (of which Peer Reviewed: 22 results) Presentation (9 results) (of which Invited: 2 results) Remarks (1 results)

  • [Journal Article] Ftx is dispensable for imprinted X-chromosome inactivation in preimplantation mouse embryos2014

    • Author(s)
      Soma M, Fujihara Y, Okabe M, Ishino F, Kobayashi S
    • Journal Title

      Sci Rep

      Volume: 4 Pages: 5181-5181

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of precise point mutation mice by footprintless genome modification2014

    • Author(s)
      Morioka Y, Fujihara Y, Okabe M
    • Journal Title

      Genesis

      Volume: 52(1) Pages: 68-77

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of an Imprinted Gene Cluster in the X-Inactivation Center2013

    • Author(s)
      Kobayashi S, Totoki Y , Soma M, Matsumoto K, Fujihara Y, Toyoda A, Sakaki Y, Okabe M , Ishino F
    • Journal Title

      PlosOne

      Volume: 8(8)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The cell biology of mammalian fertilization2013

    • Author(s)
      Okabe M
    • Journal Title

      Development

      Volume: 140(22) Pages: 4471-9

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] miR-200b and miR-429 function in mouse ovulation and are essential for female fertility2013

    • Author(s)
      Hasuwa H, Ueda J, Ikawa M, Okabe M
    • Journal Title

      Science

      Volume: 341(6141) Pages: 71-3

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa2013

    • Author(s)
      Fujihara Y, Tokuhiro K, Muro Y, Kondoh G, Araki Y, Ikawa M, Okabe M
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 110(20) Pages: 8111-6

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production of mouse pups from germline transmission-failed knockout chimeras2013

    • Author(s)
      Fujihara Y, Kaseda K, Inoue N, Ikawa M, Okabe M
    • Journal Title

      Transgenic Res

      Volume: 22(1) Pages: 195-200

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of an Imprinted Gene Cluster in the X-Inactivation Center2013

    • Author(s)
      Kobayashi S, Totoki Y , Soma M, Matsumoto K, FujiharaY, Toyoda A, Sakaki Y, Okabe M , Ishino F
    • Journal Title

      PlosOne

      Volume: 8

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GPI-anchored protein complex, LY6K/TEX101, is required for sperm migration into the oviduct and male fertility in mice.2013

    • Author(s)
      Fujihara Y, Okabe M, Ikawa M.
    • Journal Title

      Biol Reprod.

      Volume: 90

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] miR-200b and miR-429 function in mouse ovulation and are essential for female fertility.2013

    • Author(s)
      Hasuwa H, Ueda J, Ikawa M, Okabe M.
    • Journal Title

      Science

      Volume: 341 Pages: 71-73

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa.2013

    • Author(s)
      Fujihara Y, Tokuhiro K, Muro Y, Kondoh G, Araki Y, Ikawa M, Okabe M.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 110 Pages: 8111-8116

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SPACA1-deficient male mice are infertile with abnormally shaped sperm heads reminiscent of globozoospermia2012

    • Author(s)
      Fujihara Y, Satouh Y, Inoue N, Isotani A, Ikawa M, Okabe M
    • Journal Title

      Development

      Volume: 139 Issue: 19 Pages: 3583-9

    • DOI

      10.1242/dev.081778

    • NAID

      120006957401

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO12012

    • Author(s)
      Satouh Y, Inoue N, Ikawa M, Okabe M
    • Journal Title

      J Cell Sci

      Volume: 125(Pt 21) Pages: 4985-90

    • NAID

      120006957400

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mice expressing aberrant sperm-specific protein PMIS2 produce normal-looking but fertilization-incompetent spermatozoa2012

    • Author(s)
      Yamaguchi R, Fujihara Y, Ikawa M, Okabe M
    • Journal Title

      Mol Biol Cell

      Volume: 23(14) Pages: 2671-9

    • NAID

      120006957402

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tetraspanin-interacting protein IGSF8 is dispensable for mouse fertility2012

    • Author(s)
      Inoue N, Nishikawa T, Ikawa M, Okabe M
    • Journal Title

      Fertil Steril

      Volume: 98(2) Pages: 465-70

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility [corrected]2012

    • Author(s)
      Tokuhiro K, Ikawa M, Benham AM, Okabe M
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 109(10) Pages: 3850-5

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO12012

    • Author(s)
      Satouh Y, Inoue N, Ikawa M, Okabe M
    • Journal Title

      Journal of Cell Science

      Volume: 125 Pages: 4985-4990

    • DOI

      10.1242/jcs.100867

    • NAID

      120006957400

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Production of mouse pups from germline transmission-failed knockout chimeras.2012

    • Author(s)
      Fujihara Y., et al.
    • Journal Title

      Transgenic Res

      Volume: 22 Issue: 1 Pages: 195-200

    • DOI

      10.1007/s11248-012-9635-x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mice expressing aberrant sperm-specific protein PMIS2 produce normal-looking but fertilization-incompetent spermatozoa2012

    • Journal Title

      Mol Biol Cell

      Volume: 23 Issue: 14 Pages: 2671-9

    • DOI

      10.1091/mbc.e11-12-1025

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tetraspanin-interacting protein IGSF8 is dispensable for mouse fertility.2012

    • Author(s)
      Inoue N., et al.
    • Journal Title

      Fertil Steril

      Volume: 98 Issue: 2 Pages: 465-470

    • DOI

      10.1016/j.fertnstert.2012.04.029

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Formation of a thymus from rat ES cells in xenogeneic nude mouse↔rat ES chimeras2011

    • Author(s)
      Isotani A, Hatayama H, Kaseda K, Ikawa M, Okabe M
    • Journal Title

      Genes Cells

      Volume: 16(4) Pages: 397-405

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The X-linked Imprinted Gene Family Fthl17 Shows Predominantly Female Expression Following the 2-cell Stage in Mouse Embryos2010

    • Author(s)
      Kobayashi S, Fujihara Y, Mise N, Kaseda K, Abe K, Ishino F, Okabe M
    • Journal Title

      Nucleic Acids Res

      Volume: 38(11) Pages: 3672-81

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] X染色体上に発見したインプリント遺伝子のクラスター2013

    • Author(s)
      小林慎
    • Organizer
      信州生命科学サマーセミナー
    • Place of Presentation
      長野
    • Year and Date
      2013-07-23
    • Related Report
      2013 Final Research Report
  • [Presentation] X 染色体不活性化中心(Xic)領域に発見したインプリント遺伝子のクラスター2013

    • Author(s)
      小林慎, 十時泰, 相馬未來, 松本和也, 藤原祥高, 豊田敦, 榊佳之, 岡部勝, 石野史敏
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] X染色体不活性化中心(Xic)領域に発見したインプリント遺伝子のクラスター2013

    • Author(s)
      小林慎,十時泰,相馬未來, 松本和也,藤原祥高,豊田敦,榊佳之,岡部勝,石野史敏
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] X染色体上に発見したインプリント遺伝子のクラスター2013

    • Author(s)
      小林慎
    • Organizer
      信州生命科学サマーセミナー2013
    • Place of Presentation
      信州大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] マウス着床前胚において雌でのみ発現する small RNA の探索2012

    • Author(s)
      相馬未來, 十時泰, 松本和也, 藤原祥高, 豊田敦, 榊佳之, 岡部勝, 石野史敏, 小林慎
    • Organizer
      第35回日本分子生物学会年会
    • Related Report
      2013 Final Research Report
  • [Presentation] 雌雄の発生と X 染色体上のインプリント遺伝子2012

    • Author(s)
      小林 慎
    • Organizer
      第5回生殖研究若手の会
    • Related Report
      2013 Final Research Report
  • [Presentation] Discovery of female specific small RNA s in preimplantation embryos2012

    • Author(s)
      Shin Kobayashi
    • Organizer
      The 22nd CDB Meet ing RNA Sciences in Cell and Developm ental Biology II
    • Place of Presentation
      Kobe
    • Related Report
      2013 Final Research Report
  • [Presentation] Discovery of female specific small RNAs in preimplantation embryos2012

    • Author(s)
      Shin Kobayashi
    • Organizer
      The 22nd CDB Meeting RNA Sciences in Cell and Developmental Biology II
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] マウス着床前胚において雌でのみ発現するsmall RNAの探索2012

    • Author(s)
      相馬未來,十時泰,松本和也,藤原祥高,豊田敦,榊佳之,岡部勝,石野史敏,小林慎
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Remarks] 東京医科歯科大学, エピジェネティクス分野ホームページ

    • URL

      http://www.tmd.ac.jp/mri/epgn/shin_kobayashi.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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