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The development and demonstration of methodology to elucidate the sugar chain function and glycosyltransferase knockout mouse phenotype.

Research Project

Project/Area Number 23500493
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionKanazawa University

Principal Investigator

SUGIHARA Kazushi  金沢大学, 医学系, 技術専門職員 (10377418)

Co-Investigator(Kenkyū-buntansha) ASANO Masahide  金沢大学, 学際科学実験センター, 教授 (50251450)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords発生工学 / 遺伝子改変動物 / 糖鎖生物学
Research Abstract

From behavioral analyses of beta 1, 4-galactosyltransferase-2 (B4GalT-2) gene knockout mice, we have found that galactose sugar chains synthesized by B4GalT-2 have a function in the central nervous system. Therefore, we tried to generate novel knock-in mice in order to identify cells and brain regions where B4GalT-2 is involved, to obtain clues identifying functional carbohydrate molecules. We constructed two kinds of targeting vectors, one for monitoring B4GalT-2 expression by lacZ gene and the other for recovering B4GalT-2 expression conditionally. We are trying to isolate desired ES clones.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (8 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] EXTL2, a member of EXT family of tumor suppressors, controls glycosaminoglycan biosynthesis in a xylose kinase-dependent manner2013

    • Author(s)
      Nadanaka, S., Zhou, S., Kagiyama, S., Shoji, N., Sugahara, K., Sugihara, K., Asano, M.and Kitagawa, H.
    • Journal Title

      J. Biol. Chem

      Volume: 288 Issue: 13 Pages: 9321-9333

    • DOI

      10.1074/jbc.m112.416909

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Large-scale identification of target proteins of a glycosyltransferase isozyme by Lectin-IGOT-LC/MS, an LC/MS-based glycoproteomic approach2012

    • Author(s)
      Sugahara, D., Kaji, H., Sugihara, K., Asano, M., and Narimatsu, H.
    • Journal Title

      Scientific Reports

      Volume: 2 Issue: 1 Pages: 680-680

    • DOI

      10.1038/srep00680

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Essential role of endothelial class II PI3K-C2αin angiogenesis and vascular barrier function2012

    • Author(s)
      Yoshioka K, Yoshida K, Cui H, Wakayama T, Takuwa N, Okamoto Y, Du W, Qi X, Asanuma K, Sugihara K, Aki S, Miyazawa H, Biswas K, Nagakura C, Ueno M, Iseki S, Schwartz JR, Okamoto H, Sasaki T,Matsui O, Asano M, Adams HR, Takakura N, Takuwa Y.
    • Journal Title

      Nature med

      Volume: 18 Issue: 10 Pages: 1560-1569

    • DOI

      10.1038/nm.2928

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Glycoprotein hyposialylation gives rise to a nephrotic-like syndrome that is prevented by sialic acid administration in GNE V572L point-mutant mice2012

    • Author(s)
      Ito, M., Sugihara, K., Asaka, T., Toyama, T., Yo sh ih ar a, T., Furuichi, K., Wada, T. and Asano, M.
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 1 Pages: 1-13

    • DOI

      10.1371/journal.pone.0029873

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 4-galactosyltransferase-II and GlcAT-PFacilitates Biosynthesis of N-linked HNK-1Carbohydrate2011

    • Author(s)
      N. Nakagawa, T. Yoshihara, M. Asano, and S. Oka
    • Journal Title

      J. Biol. Chem

      Volume: 286(36) Issue: 36 Pages: 31337-46

    • DOI

      10.1074/jbc.m111.233353

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] GNE V572L 点変異マウスにおける腎臓の病態解析とシアル酸糖鎖の関与2011

    • Author(s)
      伊藤光俊ら
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2011 Research-status Report
  • [Presentation] Hyposialylation of glycoproteins in the UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) point mutant mice gives rise to a renal impairment that is prevented by sialic acid administration2011

    • Author(s)
      Ito, M et al.
    • Organizer
      Kidney Week 2011
    • Place of Presentation
      Pennsylvania Convention Center (USA)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://kiea.w3.kanazawa-u.ac.jp/tglab/tganimHP/Top.html

    • Related Report
      2013 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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