The cell transplant system using improved TRECK mice

• Project/Area Number: 23500496
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: SAITO Michiko 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40379558)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 再生医療 / 糖尿病 / 毒素受容体 / 再生移植

Research Abstract

The TRECK method is very useful for generating model mice and obtaining useful donor cells. However, it has been difficult to make DTR expressed in whole bodies of mice because DTR is a growth factor and overexpression of it induces a slightly abnormal phenotype. In this study, to solve these problems, we generated improved TRECK mice using a modified DT receptor with reduced growth factor activity. The improved TRECK mice showed a normal phenotype and the DTR could be expressed in whole mice bodies. This improvement is expected to extend the applicable range of the TRECK method. Furthermore, we could demonstrate that transplanted and accepted donor cells from this mouse could be removed again from the recipient mouse by DT administration to examine the effect of the transplantation.

Research Products

• [Journal Article] Identification of the redox partners of ERdj5 /JPDI, a PDI family member, from an animal tissue (2013)
  • Author(s): Kadokura H, Saito M, Tsuru A, Hosoda A, Iwawaki T, Inaba K, Kohno K
  • Journal Title: Biochemical and biophysical research communications Volume: 440(2) Pages: 245-50
  • Peer Reviewed
• [Journal Article] Generation of mouse models for type 1 diabetes by selective depletion of pancreatic beta cells using toxin receptor-mediated cell knockout (2013)
  • Author(s): Matsuoka K, Saito M, Shibata K, Sekine M, Shitara H, Taya C, Xiaohong Zhang, Takahashi A. T., Kohno K, Kikkawa Y, Yonekawa H
  • Journal Title: Biochemical and biophysical research communications Volume: 436 Pages: 400-405
  • Peer Reviewed
• [Journal Article] Negative feedback by IRE1β optimizes mucin production in goblet cells (2013)
  • Author(s): Tsuru A, Fujimoto N, Takahashi S, Saito M, Nakamura D, Iwano M, Iwawaki T, Kadokura H, Ron D, Kohno K
  • Journal Title: Proc Natl Acad Sci USA Volume: 110(8) Pages: 2864-9
  • Peer Reviewed
• [Journal Article] Fetal exposure to 2, 3, 7, 8- tetrachlorodibenzo-p-dioxin transactivates aryl hydrocarbon receptor-responsive element III in the tyrosine hydroxylase immunoreactive neurons of the mouse midbrain (2013)
  • Author(s): Tanida T, Tasaka K, Akahoshi E, Ishihara-Sugano M, Saito M, Kawata S, Danjo M, Tokumoto J, Mantani Y, Nagahara D, Tabuchi Y, Yokoyama T, Kitagawa H, Kawata M, Hoshi N
  • Journal Title: J Appl Toxicol
  • Peer Reviewed
• [Journal Article] Generation of mouse models for type 1 diabetes by selective depletion of pancreatic beta cells using toxin receptor-mediated cell knockout. (2013)
  • Author(s): Matsuoka, K., Saito, M., Shibata, K., Sekine, M., Shitara, H., Taya, C., Zhang, X., Takahashi, T, a., Kohno, K., Kikkawa, Y., Yonekawa, H
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 436 Issue: 3 Pages: 400-405
  • DOI: 10.1016/j.bbrc.2013.05.114
  • Peer Reviewed
• [Journal Article] Identification of the redox partners of ERdj5/JPDI, a PDI family member, from an animal tissue. (2013)
  • Author(s): Kadokura, H., Saito, M., Tsuru, A., Hosoda, A., Iwawaki, T., Inaba, K., & Kohno, K.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 440 Issue: 2 Pages: 245-250
  • DOI: 10.1016/j.bbrc.2013.09.063
  • Peer Reviewed
• [Journal Article] Fetal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin transactivates aryl hydrocarbon receptor-responsive element III in the tyrosine hydroxylase immunoreactive neurons of the mouse midbrain (2013)
  • Author(s): Tanida T, Tasaka K, Akahoshi E, Ishihara-Sugano M, Saito M, Kawata S, Danjo M, Tokumoto J, Mantani Y, Nagahara D, Tabuchi Y, Yokoyama T, Kitagawa H, Kawata M, Hoshi N
  • Journal Title: Journal of Applied Toxicology Volume: Jan 8 Issue: 2 Pages: 1099-1263
  • DOI: 10.1002/jat.2839
  • Peer Reviewed / Open Access
• [Journal Article] Negative feedback by IRE1β optimizes mucin production in goblet cells. (2013)
  • Author(s): Tsuru, Akio
  • Journal Title: Proc Natl Acad Sci USA Volume: 110 Issue: 8 Pages: 2864-2869
  • DOI: 10.1073/pnas.1212484110
  • Peer Reviewed
• [Journal Article] A novel mammalian ER-located J-protein, DNAJB14, can accelerate ERAD of misfolded membrane proteins (2012)
  • Author(s): Kadokura H, Yamamoto Y, Takeuchi M, Saito M, Tsuru A, Kohno K
  • Journal Title: Cell Structure and Function Volume: 37(2) Pages: 177-87
  • NAID: 130004137586
  • Peer Reviewed
• [Journal Article] Comparative study of transplantation of hepatocytes at various differentiation stages into mice with lethal liver damage (2012)
  • Author(s): Kamimura R, Ishii T, Sasaki N, Kajiwara M, Machimoto T, Saito M, Kohno K, Suemori H, Nakatsuji N, Ikai I, Yasuchika K, and Uemoto S
  • Journal Title: Cell Transplantation Volume: 21(11) Pages: 2351-2362
  • Peer Reviewed
• [Journal Article] Comparative study of transplantation of hepatocytes at various differentiation stages into mice with lethal liver damage (2012)
  • Author(s): Ryo Kamimura, Takamichi Ishii, Naoya Sasaki, Masatoshi Kajiwara, Takafumi Machimoto, Miwa Saito, Kohno K, Hirofumi Suemori, Norio Nakatsuji, Iwao Ikai, Kentaro Yasuchik, Shinji Uemoto
  • Journal Title: Cell Transplant Volume: 21(11) Issue: 11 Pages: 2351-62
  • DOI: 10.3727/096368912x636957
  • Peer Reviewed
• [Presentation] 細胞系譜の異なる細胞から膵 ・ 細胞へのダイレクトリプログラミング (2014)
  • Author(s): 森田この美, 斉藤美知子, 河野憲二
  • Organizer: 第13回日本再生医療学会
  • Place of Presentation: 京都国際会館
• [Presentation] 細胞系譜の異なる細胞から膵β細胞へのダイレクトリプログラミング (2014)
  • Author(s): 森田この美、斉藤美知子、河野憲二
  • Organizer: 第13回日本再生医療学会
  • Place of Presentation: 京都国際会館、京都市左京区宝ヶ池
• [Presentation] Diabetes-New Insights into Mechanism of Disease and its Treatment (J6) (2013)
  • Author(s): Yuichi Tuchiya, Michiko Saito, Takao Iwawaki Jun-ichi Miyazaki, Kenji Kohno
  • Organizer: IRE1α is indispensable for the efficient production of insulin in pancreatic β cell Keystone Symposia
  • Place of Presentation: Keystone Resort (U.S.A)
  • Year and Date: 2013-01-30
• [Presentation] 小胞体ストレスセンサー IRE1 αの効率的なインスリンの成熟への役割 (2013)
  • Author(s): Yuichi Tuchiya, Michiko Saito, Takao Iwawaki Jun-ichi Miyazaki, Kenji Kohno
  • Organizer: 第65回 日本細胞生物学会大会
  • Place of Presentation: 名古屋
• [Presentation] 小胞体ストレスセンサーIRE1α の効率的なインスリンの成熟への役割 (2013)
  • Author(s): Yuichi Tuchiya, Michiko Saito, Takao Iwawaki Jun-ichi Miyazaki, Kenji Kohno
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: ウインクあいち（愛知県産業労働センター）、名古屋市中村区
• [Presentation] Negative feedback by IRE1β optimizes mucin production in goblet cells (2012)
  • Author(s): Akio Tsuru, Naoko Fujimoto, Satsuki Takahashi, Daisuke Nakamura, Michiko Saito, Rina Nagai, Megumi Iwano, Takao Iwawaki, David Ron, Kenji Kohno
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2012-05-30
• [Presentation] Negative feedback by IRE1β optimizes mucin production in goblet cells (2012)
  • Author(s): Akio Tsuru, Michiko Saito, Megumi Iwano, Takao Iwawaki, David Ron, Kenji Kohno
  • Organizer: Cold spring harbor meeting
  • Place of Presentation: Cold spring harbor , U.S.A
  • Year and Date: 2012-05-04
• [Presentation] Mechanism of diabetes development due to dysfunction of unfolded protein response (2012)
  • Author(s): 斉藤美知子, 土屋雄一, 岩脇隆夫, 森和俊, 河野憲二
  • Organizer: 第35回 日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] The Role of the Unfolded Protein Response in Pancreatic β cells (2012)
  • Author(s): Yuichi Tsuchiya, Michiko Saito, Takao Iwawaki, Jun-ichi Miyazaki, Kazutoshi Mori, Kenji Kohno
  • Organizer: 日本農芸化学会2012年度京都大会
  • Place of Presentation: 京都女子大学, 聖護院, ウェスティン都ホテル京都
• [Presentation] Roles of the unfolded protein response pathways in pancreatic β cells (2011)
  • Author(s): Yuichi Tsuchiya, Michiko Saito, Jun-ichi Miyazaki, Takao Iwawaki, Kenji Kohno
  • Organizer: Cold Spring Harbor Conferences Asia, Protein Homeostasis in Health and Disease 2011
  • Place of Presentation: Suzhou Industrial Park 215123 China
  • Year and Date: 2011-09-27
• [Presentation] Role of the unfolded protein response pathway in pancreatic β cells (2011)
  • Author(s): 斉藤美知子, 土屋雄一, 岩脇隆夫, 森和俊, 河野憲二
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Mechanism of diabetes development due to dysfunction of unfolded protein response (2011)
  • Author(s): 斉藤美知子、土屋雄一、岩脇隆夫、森和俊、河野憲二
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Negative feedback by IRE1β optimizes mucin production in goblet cells
  • Author(s): Akio Tsuru, Michiko Saito, Megumi Iwano, Takao Iwawaki, David Ron, Kenji Kohno
  • Organizer: Cold spring harbor meeting
  • Place of Presentation: Cold spring harbor
• [Presentation] Negative feedback by IRE1β optimizes mucin production in goblet cells
  • Author(s): Akio Tsuru, Naoko Fujimoto, Satsuki Takahashi, Daisuke Nakamura, Michiko Saito, Rina Nagai, Megumi Iwano, Takao Iwawaki, David Ron, Kenji Kohno
  • Organizer: 第45回日本発生生物学会・第64回日本細胞生物学会合同大会
  • Place of Presentation: 兵庫県神戸市
• [Presentation] The Role of the Unfolded Protein Response in Pancreatic β cells
  • Author(s): Yuichi Tsuchiya, Michiko Saito, Takao Iwawaki, Jun-ichi Miyazaki, Kazutoshi Mori, Kenji Kohno
  • Organizer: Kyeystone Symposia Meeting
  • Place of Presentation: Keystone, Colorado, USA
• [Presentation] 小胞体ストレス応答不全による糖尿病発症機構の解明
  • Author(s): 斉藤美知子、土屋雄一、岩脇隆夫、森和俊、河野憲二
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡県福岡市
• [Remarks]
  • URL: http://bsw3.naist.jp/kouno/kouno.html