Effects of bone marrow mesenchymal stem cells on encephalopathy caused by Shiga toxin 2c-producing Escherichia coli infection in mice
| Project/Area Number |
23500497
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tottori University (2012-2013)
Kyushu University (2011) |
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Principal Investigator |
FUJII Jun 鳥取大学, 医学部, 教授 (60271441)
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| Co-Investigator(Renkei-kenkyūsha) |
DEZAWA Mari 東北大学, 大学院・医学系研究科, 教授 (50272323)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 腸管出血大腸菌 / 脳症 / 再生医療 / Muse細胞 / 骨髄間葉系幹細胞 / 腸管出血性大腸菌 / EHEC / ベロ毒素 / Stx2 / Muse / MSCs |
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| Research Abstract |
We transplanted Mutilineage-differenting stress-enduring (Muse) cells or non-Muse cells intravenously to the mouse model that impaired blood brain barrier caused by an oral infection of Stx2c-producing E. coli (E32511). We investigated the effects of Muse and non-Muse cells on encephalopathy caused by E32511 infection. Only Muse cells engrafted in the pons and medulla and non-Muse cells did not engraft in any area of the whole brain. As the results, caspase-3 activation was statistically decrease in the mouse brain transplanted Muse cells in the E32511-infected mice. The water channel aquaporin 4 (AQP4) is located in the perivascular end-feet of astrocytes (ASTs) in the brain. Furthermore, reactive ASTs and down-regulation of AQP4were not detected in the brain transplanted Muse cells although reactive ASTs and down-regulation could be detectable in the brain transplanted non-Muse cells.
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Report
(4 results)
Research Products
(33 results)
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[Presentation] Risk factors for hemolytic uremic syndrome caused by Shiga toxin-producing Escherichia coli infection in Japan2012
Author(s)
Fujii J, Mizoue T, Nakada Y, Ugajin S, Naya Y, Nakamura T, Saitoh K, Maruyama Y, TadaY, Okabe N and Yoshida S
Organizer
8th International Symposium on Shiga toxin (Verocytotoxin)- producing Escherichia coli infection
Place of Presentation
Amsterdam, The Netherlands
Year and Date
2012-05-08
Related Report
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