| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
We transplanted Mutilineage-differenting stress-enduring (Muse) cells or non-Muse cells intravenously to the mouse model that impaired blood brain barrier caused by an oral infection of Stx2c-producing E. coli (E32511). We investigated the effects of Muse and non-Muse cells on encephalopathy caused by E32511 infection. Only Muse cells engrafted in the pons and medulla and non-Muse cells did not engraft in any area of the whole brain. As the results, caspase-3 activation was statistically decrease in the mouse brain transplanted Muse cells in the E32511-infected mice. The water channel aquaporin 4 (AQP4) is located in the perivascular end-feet of astrocytes (ASTs) in the brain. Furthermore, reactive ASTs and down-regulation of AQP4were not detected in the brain transplanted Muse cells although reactive ASTs and down-regulation could be detectable in the brain transplanted non-Muse cells.
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