Development of a method for simultaneous detection of 4 opportunistic pathogen in mouse
| Project/Area Number |
23500500
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 診断 / 検査 / マウス / 日和見感染症 / 等温遺伝子増幅法 / ノロウイルス / 遺伝子増幅法 / ＬＡＭＰ法 / 核酸増幅検査 / LAMP法 / マウスノロウイルス / マウス肝炎ウイルス / 微生物モニタリング / 遺伝子診断 / PCR法 |
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| Research Abstract |
Opportunistic organisms, which rarely cause infections in immunocompetent mice, can cause infections in immunocompromised mice. Since opportunistic infection may alter research results using immunocompromised mice, health certificate showing the immunocompromised mouse colony is free from specific opportunistic pathogens is important for biomedical researchers.
The purpose of this study is development of simple and rapid diagnostic loop-mediated isothermal amplification (LAMP) methods for detecting 4 opportunistic pathogens: Staphylococcus aureus (Sa), mouse hepatitis virus (MHV), murine norovirus (MNV), and Pasteurella pneumotropica (Pp). Reverse transcription-LAMP methods for the detection of MHV and MNV have been developed and published in the Journal of Virological Methods. LAMP methods for the detection of antiseptic- and methicillin-resistant Sa had also been developed. However, LAMP method for the detection of Pp has been under construction.
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Report
(4 results)
Research Products
(20 results)
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[Journal Article] Detection of murine norovirus by reverse transcription loop-mediated isothermal amplification2014
Author(s)
Hanaki, K., Ike, F., Kajita, A., Yasuno, W., Yanagiba, M., Goto, M., Sakai, K., Ami, Y., Kyuwa, S
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Journal Title
J Virol Methods
Volume: 204C
Pages: 17-24
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus2014
Author(s)
Hanaki, K., Ike, F., Kajita, A., Yasuno, W., Yanagiba, M., Goto, M., Sakai, K., Ami, Y., Kyuwa, S
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Journal Title
PLoS One
Volume: 9
Issue: 5
Pages: e98108-e98108
DOI
Related Report
Peer Reviewed / Open Access
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