| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
Microinsemination is a technique to deliver male germ cells directly into oocytes, and is a very effective technique to fertilize oocytes consistently, even male germ cells that are unable to fertilize oocytes under conventional in vovo and in vitro conditions. Although offspring can be obtained by primary spermatocyte injection in mice, its efficiency is still low (2-4%) compared with that by spermatid (30%) or by spermatozoa (40-50%). To know whether the poor full-term development, primary spermatocyte injected oocytes were monitored by live-cell imaging techniques. The gap of the chromosome division timing was observed between male and female chromosomes. And a histone, which are globally deacetylated in mammalian oocytes during meiosis, was remained acetylated in primary spermatocyte injected oocytes. Thus, inadequate histone deacetylation may be caused chromosome abnormalities and low oocyte developments.
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