| Budget Amount *help |
¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2012: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
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| Research Abstract |
In order to clarify the initial activation of human platelet in forming thrombi on collagen fibrils or VWF, localizations of intra-cytosolic calcium ion in an individual platelet were imaged in real time using an ultrafast confocal laser microscope. Images of a single platelet were divided into 16 segments (4x4) the intra-cytosolic calcium ion concentrations ([Ca2+]i ) in each segment were measured with software Image J. The [Ca2+]i of individual platelets transiently increased after they first bound to the VWF or the collagen fibrils. The increase in [Ca2+]i started at the center division of each individual platelet under blood flow conditions, although there were no significant difference in [Ca2+]i division in activated platelets. In this study we established the methods of platelet Ca2+ localization under various activated states when platelets bound with VWF or collagen under blood flow conditions.
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