| Project/Area Number |
23500540
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NAGAI Akiko 東京医科歯科大学, 生体材料工学研究所, 准教授 (40360599)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Kimihiro 東京医科歯科大学, 生体材料工学研究所, 教授 (70174670)
NAKAMURA Miho 東京医科歯科大学, 生体材料工学研究所, 准教授 (40401385)
HORIUCHI Naohiro 東京医科歯科大学, 生体材料工学研究所, 助教 (90598195)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | バイオマテリアル / 表面改変 / ステント材料 / 静電気力 / 血管内皮細胞 / 血小板 / 金属材料 / 材料ー細胞相互作用 / バイオセラミックス / ステント / コーティング / 血管適合性 |
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| Research Abstract |
We report on the surface modifications of metal substrates for stent, such as cobalt-chrome alloy and titanium alloy, for endothelialization at their surfaces by anodic oxidation treatment and femtosecond laser irradiation, respectively. We evaluated the surfaces using endothelial cells attachment test and platelet adhesion test in vitro. These results show their possibilities to be useful stent surfaces which promote attachments of endothelial cells for inhibiting restenosis and suppress adhesion of platelet inducing thrombogenesis.
Next, we investigated the mechanism of interactions between electrostatic force of the material surfaces and cell behaviors using an atomic force microscopy, a quartz crystal microbalance with dissipation monitoring, and a fluorescence microscope. The conformational changes of the serum protein modulate its configuration when adsorbed to the material surfaces and the initial cell behavior of cells on the surface with pre-adsorbed the protein.
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