| Project/Area Number |
23500543
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Osaka City University |
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Principal Investigator |
TACHIBANA Akira 大阪市立大学, 大学院工学研究科, 准教授 (80305614)
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| Co-Investigator(Kenkyū-buntansha) |
TANABE Toshizumi 大阪市立大学, 大学院・工学研究科, 教授 (20315972)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 徐放システム / FGF2 / キチン / キチンゲル / キチン結合ドメイン / リゾチーム応答性徐放 / chitin binding domain / BMP2/7 / キトサン / セルロース / セルロース結合ドメイン / pH感受性 |
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| Research Abstract |
Chitin gel was obtained by the acetylation of chitosan in solution. Basic growth factors did not bind to non treated chitin gel, whereas tightly bound to carboxymethylated chitin gel. Chitin binding domain-FGF-2 was bound to chitin gel and not released by PBS washing. Chitin binding domain-FGF-2 was released by degradation of chitin gel with lysozyme. During degradation of chitin gel with lysozyme, chitin fragment was released at a burst, but chitin binding domain-FGF-2 was not. Most of chitin binding domain-FGF-2 that was released from gel were re-bound to the gel, only low amount of FGF-2 was released out. Therefore, ideal controlled release without a burst release was realized.
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