Development of minimally invasive strategy for in-stent restenosis for peripheral arterial disease

• Project/Area Number: 23500565
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical systems
• Research Institution: Keio University
• Principal Investigator: OBARA HIDEAKI 慶應義塾大学, 医学部, 講師 (20276265)
• Research Collaborator: MATSUDA Yuko 慶應義塾大学, 医学部, 特任助教 (90534537)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 動脈硬化 / 内膜肥厚 / ステント / 再狭窄 / フリーラジカル / 低侵襲治療 / 外部エネルギー

Research Abstract

Aim of this study is to develop minimally invasive strategy for preventing in-stent restenosis of peripheral arterial disease.
The generation of heat was obtained by magnetic field irradiation to a nickel-titanium alloy (nitinol) stent at 200kHz or 900kHz/4mT in in vitro, but we could not reach to the stage of animal experiments due to construction of magnetic field generator. The generation of the free radicals was observed by ultrasound irradiation to a nitinol stent at 500kHz or 1MHz. Free radicals were effective to suppress the in-stent stenosis on xenograft model (subcutaneous tumor model). WHHLMI rabbit exhibits hereditary hyperlipidemia and arteriosclerosis. In the rabbit intimal thickening model, the main artery of the WHHLMI rabbit rubbed with Fogarty catheter, and then, nitinol stent was inserted after two weeks from the treatment. There was no restenosis after one month from insersion, thereby the model was extended up to six months and are currently being analyzed.