| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
In this study, we investigated the sonodynamically induced antitumor effect of functionalized fullerenes. The antitumor effects of functionalized fullerenes in combination with ultrasound at 2 MHz were screened. The ultrasonically induced damage on isolated sarcoma 180 cells was enhanced significantly with polyhydroxy fullerenes (PHF) or pyrrolidine tris-acid fullerenes (PTF). In the experimental treatment, the combined with ultrasonic exposure and PHF inhibited the growth of the inoculated colon 26 tumors. The significant reduction by histidine of ultrasonically induced cell damage enhanced by PHF suggests that the enhancement was due to sonodynamic generation of active oxygen such as singlet oxygen by functionalized fullerenes. The involvement of singlet oxygen was also confirmed by ESR spectometory. These results support the possibility of clinical application of sonodynamic treatment using water soluble functionalized fullerene.
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