Development of Diagnosis Device for Alzheimer's Disease Using Amyloid beta Fibrillization Promoting Peptide

• Project/Area Number: 23500571
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical systems
• Research Institution: Konan University
• Principal Investigator: FUJII SATOSHI 甲南大学, フロンティアサイエンス学部, 教授 (80271518)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: アルツハイマー型認知症 / アミロイドβ / プロテインチップ / 電気化学 / センサー / 水晶振動子マイクロバランス法 / バイオマーカー / 診断システム / アルツハイマー病 / 水晶振動子マイクロバランス / アミロイドβペプチド / 診断装置

Outline of Final Research Achievements

Amyloid beta peptide (A beta) is a potential biomarker to evaluate the risk of Alzheimer's disease. Our purpose in this study was to develop a preclinical diagnostic device for Alzheimer's disease with ultra high sensitivity, ease, and high-through put, and at low cost. Using an A beta aggregation promoting peptide which was developed by us, we can detect ~10 nM of A beta quantitatively within 1 hour. We are now trying to improve the sensitivity to detect plasma A beta and put it to practical use.

Research Products

• [Journal Article] De Novo Designed Nitric Oxide Sensor Protein Consisted of Iron Complex-Peptide Conjugate (2012)
  • Author(s): Hiroshi Miyazaki, Kenji Usui, and Satoshi Fujii
  • Journal Title: Peptide Science Volume: ー Pages: 303-304
  • NAID: 10030204443
  • Peer Reviewed
• [Journal Article] A Novel Nitric Oxide Sensor Using Fluorescent Peptides Attached to Iron Complexes (2011)
  • Author(s): Hiroshi Miyazaki, Kenji Usui, and Satoshi Fujii
  • Journal Title: Peptides: A Building Bridges The Proceedings of the Twenty-Second American Peptide Symposium Volume: ー Pages: 224-225
• [Presentation] アミロイドβ線維化促進ペプチドを用いたアミロイドβの電気化学的検出 (2013)
  • Author(s): 園大樹、藤井敏司
  • Organizer: 第７回バイオ関連化学シンポジウム
  • Place of Presentation: 名古屋大学
• [Presentation] アミロイドβペプチドの線維化促進メカニズムに関する研究 (2013)
  • Author(s): 園大樹・藤井敏司
  • Organizer: 第93回日本化学会春季年会
  • Place of Presentation: 立命館大学びわこ草津キャンパス
• [Presentation] アミロイドβ凝集促進ペプチドの凝集促進メカニズムに関する研究 (2012)
  • Author(s): 園大樹・松田春香・藤井敏司
  • Organizer: 第６回バイオ関連化学合同シンポジウム
  • Place of Presentation: 北海道大学
• [Presentation] Novel Nitric Oxide Sensor Using Fluorescent Peptides Attached to Iron Complexes (2011)
  • Author(s): Hiroshi Miyazaki, Kenji Usui, and Satoshi Fujii
  • Organizer: 22nd American Peptide Symposium
  • Place of Presentation: San Diego, CA, USA
• [Presentation] A Novel Nitric Oxide Sensor Using Iron Complex with Dithiocarbamate-Fluorescent Peptide Conjugate That Forms A Three-Helix Bundle Structure (2011)
  • Author(s): Hiroshi Miyazaki, Kenji Usui, and Satoshi Fujii
  • Organizer: 15th International Conference on Biological Inorganic Chemistry
  • Place of Presentation: UBC, Vancouver, Canada
• [Presentation] De Novo Designed Nitric Oxide Sensor Protein Consisted of Iron Complex-Peptide Conjugate (2011)
  • Author(s): 宮崎洋、臼井健二、藤井敏司
  • Organizer: 第４８回ペプチド討論会
  • Place of Presentation: 札幌コンベンションセンター（北海道）
• [Presentation] 錯体化学を医療に活かす ～遙かなる頂を目指して～ (2011)
  • Author(s): 藤井敏司
  • Organizer: 第４５回錯体化学若手の会近畿地区勉強会(招待講演)
  • Place of Presentation: 大阪市立大学（大阪府）