| Project/Area Number |
23500835
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
KUGA Keisuke 筑波大学, 医学医療系, 教授 (60241816)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAI Satoshi 筑波大学, 医学医療系, 講師 (30282362)
島野 仁 筑波大学, 医学医療系, 教授 (20251241)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIMANO Hitoshi 筑波大学, 医学医療系, 教授 (20251241)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 高血圧 / 線維化 / 炎症反応 / 細胞外マトリックス / 心筋線維化 / ケモカイン / SREBP-1 |
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| Research Abstract |
We evaluated the role of a transcription factor, SREBP-1, in the development of hypertesive heart disease. We continuously administrated the vasocontractile agent angiotensin II (Ang II) to the SREBP-1-knockout (KO) mice and wild type mice. The wild mice showed the marked fibrous changes around the coronary vasculature in the heart, whereas KO showed the slight changes. We analyzed the alteration of cardiac gene expression by DNA microarray system and confirmed by real-time RT-PCR. The gene groups for extracellular matrix and inflammatory chemokine and cytokine were significantly changed in the wild mice; however, those were suppressed in the KO mice. These findings suggest that SREBP-1 is involved in the development of fibrous changes seen in the hypertensive heart disease.
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