Study on physiological function of the bivalve mainly on lipid metabolism

• Project/Area Number: 23500931
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Oita University
• Principal Investigator: MOCHIZUKI Satoshi 大分大学, 教育福祉科学部, 教授 (80210087)
• Co-Investigator(Kenkyū-buntansha): ODA Hiroaki 名古屋大学, 生命農学研究科, 准教授 (20204208) ; YAMADA Koji 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (00253469)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: タイワンシジミ / コレステロール代謝 / 脂質画分 / タンパク質画分 / マイクロアレイ / 遺伝子発現 / 構造決定 / セラミド / コレステロール

Research Abstract

We examined effects of the Freshwater Clam (Corbicula fluminea) on cholesterol metabolism. The structure of the active ingredient in the lipid fraction of the heated water extract of the Freshwater Clam (Corbicula fluminea) was a ceramide phosphoramidate molecular species.
We extracted RNA from the liver of rat given the lipid fraction or the protein fraction. We performed microarray analysis using all RNA and analyzed bioinformatics. Promotion of the cholesterol catabolism and remarkable depression of activities of enzymes of fatty acid synthesis were seen in the rat given the lipid fraction. In addition, it was suggested that the lipid fraction acted on processing of the protein of SREBP. Promotion of the cholesterol catabolism was also seen in the rat given the protein fraction.

Research Products

• [Journal Article] Lipid components prepared from a freshwater Clam (Corbicula fluminea) extract ameliorate in hypercholesterolaema in rats fed high-cholesterol diet (2013)
  • Author(s): T. Chijimatsu, M. Umeki, Y. Kataoka, S. Kobayashi, K. Yamada, H. Oda, and S. Mochizuki
  • Journal Title: Food Chem Volume: 136 Pages: 328-334
• [Journal Article] Freshwater Clam Extract Ameliorates Triglyceride and Cholesterol Metabolism through the Expression of Genes Involved in Hepatic Lipogenesis and Cholesterol Degradation in Rats (2013)
  • Author(s): T. Laurent, Y. Okuda, T. Chijimatsu, M. Umeki, S. Kobayashi, Y. Kataoka, I. Tatsuguchi, S. Mochizuki, and H. Oda
  • Journal Title: Evidence-Based Complementary and Alternative Medicine
• [Journal Article] 二枚貝, 特にシジミの機能性因子に関する研究 (2013)
  • Author(s): 千々松武司, 山田耕史, 小田裕昭, 望月 聡
  • Journal Title: New Food Industry Volume: 55, (8) Pages: 23-31
  • NAID: 40019762139
• [Journal Article] 二枚貝、特にシジミの機能性因子に関する研究 (2013)
  • Author(s): 千々松武司、山田耕史、小田裕昭、望月 聡
  • Journal Title: New Food Industry Volume: 55 Pages: 23-31
  • NAID: 40019762139
  • Peer Reviewed
• [Journal Article] Lipid components prepared from a freshwater Clam (Corbicula fluminea) extract ameliorate in hypercholesterolaema in rats fed high-cholesterol diet. (2013)
  • Author(s): T.Chijimatsu, M.Umeki, Y.Kataoka, S.Kobayashi, K.Yamada, H.Oda, and S.Mochizuki
  • Journal Title: Food Chem. Volume: 136 Pages: 328-334
  • Peer Reviewed
• [Journal Article] Freshwater Clam Extract Ameliorates Triglyceride and Cholesterol Metabolism through the Expression of Genes Involved in Hepatic Lipogenesis and Cholesterol Degradation in Rats (2013)
  • Author(s): T.Laurent, Y.Okuda, T.Chijimatsu, M.Umeki, S.Kobayashi, Y.Kataoka, I.Tatsuguchi, S.Mochizuki, and H.Oda
  • Journal Title: Evidence-Based Complementary and Alternative Medicine Volume: Article ID 830684
  • Peer Reviewed
• [Journal Article] Lipid components prepared from a freshwater clam (Corbicula fluminea) extract ameliorate hypercholesterolemia in rats fed high-cholesterol diet. (2013)
  • Author(s): Chijimatsu, T., Umeki, M., Kataoka, Y., Kobayashi, S,, Yamada, K., Oda, H. and Mochizuki, S.
  • Journal Title: Food Chem. Volume: 136 Pages: 328-334
  • Peer Reviewed
• [Journal Article] Freshwater clam extract ameliorates triglyceride and cholesterol metabolism through the expression of genes involved in hepatic lipogenesis and cholesterol degradation in rats. (2013)
  • Author(s): Laurent, T., Okuda, Y., Chijimatsu, T., Umeki, M., Kobayashi, S., Kataoka, Y., Tatsuguchi, I., Mochizuki, S. and Oda, H.
  • Journal Title: Evidence-Based Complementary and Alternative Volume: 10 Pages: 1-10
  • DOI: 10.1155/2013/830684
  • Peer Reviewed
• [Presentation] シジミ抽出物中脂質およびタンパク質はクロレトン誘導脂肪性肝炎を抑制する (2014)
  • Author(s): 梅木美樹, 千々松武司, 安 美珠, 片岡裕太郎, 小林 悟, 山田耕史, 小田裕昭, 望月 聡
  • Organizer: 第68回日本栄養・食糧学会大会
• [Presentation] シジミ抽出物中資質およびタンパク質はクロレトン誘導脂肪性肝炎を抑制する (2014)
  • Author(s): 梅木美樹、千々松武司、安美珠、片岡裕太郎、小林悟、山田耕史、小田裕昭、望月聡
  • Organizer: 第68回日本栄養・食糧学会大会
  • Place of Presentation: 酪農学園大学
• [Presentation] タイワンシジミ抽出物は脂肪, コレステロールおよび鉄負荷食により誘導される脂肪肝および肝障害を抑制する (2013)
  • Author(s): 千々松武司, 梅木美樹, 望月 聡
  • Organizer: 第67回日本栄養・食糧学会大会
• [Presentation] タイワンシジミ抽出物は脂肪、コレステロールおよび鉄負荷食により誘導される脂肪肝および肝障害を抑制する (2013)
  • Author(s): 千々松武司，梅木美樹，望月 聡
  • Organizer: 第67回日本栄養・食糧学会大会
  • Place of Presentation: 名古屋大学
• [Presentation] タイワンシジミ抽出物による脂質代謝改善機構と機能性成分に関するトランスクリプトミクス解析 (2011)
  • Author(s): 小林 悟, トマローラン, 片岡裕太郎, 梅木美樹, 千々松武司, 山田耕史, 望月 聡, 小田裕昭
  • Organizer: 第65回日本栄養・食糧学会大会
• [Presentation] タイワンシジミ抽出物による脂質代謝改善機構と機能性成分に関するトランスクリプトミクス解析 (2011)
  • Author(s): 小林 悟，トマローラン，片岡裕太郎，梅木美樹，千々松武司，山田耕史，望月 聡，小田裕昭
  • Organizer: 第65回日本栄養・食糧学会
  • Place of Presentation: お茶の水女子大学（東京）
• [Remarks] 研究業績を公表(ホームページ等)
  • URL: http://kitchom.ed.oita-u.ac.jp/diet/