Study on nutritional property and diabetes prevention effects of Silkworm pupae (Bombyx mori)

• Project/Area Number: 23500947
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Iida Women's Junior College
• Principal Investigator: TOMOTAKE Hiroyuki 飯田女子短期大学, その他部局等, 教授 (90300136)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 蚕サナギ / 糖質分解酵素阻害作用 / 血糖上昇抑制作用 / 血糖上昇抑制 / アンギオテンシンI変換酵素 / 溶解性 / 脱脂 / イナゴ / 消化性 / ラジカル補足活性 / 抗菌活性 / 成分分析 / 二糖類分解酵素阻害活性

Research Abstract

This study was performed to evaluate the nutritional value and bioactive component of silkworm pupase (Bombyx mori) and the alfa-glucosidase inhibitory effect of water extracts of that. Hot water extract of silkworm pupase inhibited angiotensin-converting enzyme, porcin panreatic alfa-glucosidase and an increase of blood glucose level in rats. Phosphate buffer extract of silkworm pupase has DPPH radical scavenging activity. Methanol extract of silkworm pupase has antibacterial activity against Staphylococcus aureus. These results suggest that silkworm pupase is a new source of bioactive components and alfa-glucosidase inhibitor.

Research Products

• [Journal Article] Treatment with buckwheat bran extract prevents the elevation of serum triglyceride levels and fatty liver and in KK-Ay mice (2014)
  • Author(s): Hosaka T, Sasaga S, Yamasaka Y, Nii Y, Edazawa K, Tsutsumi R, Shuto E, Okahisa N, Iwata S, Tomotake T, Sakai T
  • Journal Title: J Med Inves Volume: 61(印刷中) Pages: 3-4
  • NAID: 130004822740
• [Journal Article] Treatment with buckwheat bran extract prevents the elevation of serum triglyceraide levels and fatty liver in KK-Ay mice (2014)
  • Author(s): Hosaka T, Sasaga S, Yamasaka Y, Nii Y, Edazawa K, Tsutsumi R, Shuto E, Okahisa N, Iwata S, Tomotake H and Sakai T
  • Journal Title: J Med Inves Volume: 61(3-4)
  • Peer Reviewed
• [Journal Article] Burdock fermented by Aspergillus awamori elevates cecal Bifidobacterium, and reduces fecal deoxycholic acid and adipose tissue weight in rats fed a high-fat diet (2013)
  • Author(s): Okazaki Y, Sitanggang NV, Sato S, Ohnishi N, Inoue J, Iguchi T, Watanabe T, Tomotake H, Harada K, Kato N
  • Journal Title: Biosci Biotechnol Biochem Volume: 77(1) Pages: 53-7
  • NAID: 10031164691
• [Journal Article] Tempe consumption modulates fecal secondary bile acids, mucins, immunoglobulin A, enzyme activities, and cecal microflora and organic acids in rats (2013)
  • Author(s): Utama Z, Okazaki Y, Tomotake H, Kato N
  • Journal Title: Plant Foods Hum Nutr Volume: 68(2) Issue: 2 Pages: 177-183
  • DOI: 10.1007/s11130-013-0357-x
  • Peer Reviewed
• [Journal Article] Burdock fermented by Aspergillus awamori elevates cecal Bifidobacterium, and reduces fecal deoxycholic acid and adipose tissue weight in rats fed a high-fat diet. (2013)
  • Author(s): Okazaki Y, Sitanggang NV, Sato S, Ohnishi N, Inoue J, Iguchi T, Watanabe T, Tomotake H, Harada K, Kato N
  • Journal Title: Biosci Biotechnol Biochem Volume: 77 Pages: 53-57
  • NAID: 10031164691
  • Peer Reviewed
• [Journal Article] An autoclave treatment reduces the solubility and antigenicity of an allergenic protein found in buckwheat flour (2012)
  • Author(s): Tomotake H, Yamazaki R, Yamato M.
  • Journal Title: J Food Prot Volume: 75 Issue: 6 Pages: 1172-1175
  • DOI: 10.4315/0362-028x.jfp-11-368
  • Peer Reviewed
• [Journal Article] Consumption of vitamin B_6 reduces fecal ratio of lithocholic acid to deoxycholic acid, a risk factor for colon cancer, in rats fed a high-fat diet (2012)
  • Author(s): Okazaki Y, Utama Z, Suidasari S, Zhang P, Yanaka N, Tomotake H, Sakaguchi E, Kato N
  • Journal Title: J Nutr Sci Vitaminol (Tokyo) Volume: 58(5) Pages: 366-70
• [Journal Article] Consumption of vitamin B₆ reduces fecal ratio of lithocholic acid to deoxycholic acid, a risk factor for colon cancer, in rats fed a high-fat diet. (2012)
  • Author(s): Okazaki Y, Utama Z, Suidasari S, Zhang P, Yanaka N, Tomotake H, Sakaguchi E, Kato N.
  • Journal Title: Nutr Sci Vitaminol (Tokyo) Volume: 58 Pages: 366-370
  • NAID: 130003368130
  • Peer Reviewed
• [Journal Article] Consumption of a resistant protein, sericin, elevates fecal immunoglobulin A, mucins, and cecal organic acids in rats fed a high-fat diet (2011)
  • Author(s): Okazaki Y, Tomotake H, Tsujimoto K, Sasaki M, Kato N
  • Journal Title: J Nutr Volume: 141(11) Issue: 11 Pages: 1975-81
  • DOI: 10.3945/jn.111.144246
• [Journal Article] Extracts of common buckwheat bran prevent sucrose digestion (2011)
  • Author(s): Hosaka T, Nii Y, Tomotake H, Ito T, Tamanaha A, Yamasaka Y, Sasaga S, Edazawa K, Tsutsumi R, Shuto E, Okahisa N, Iwata S, Sakai T
  • Journal Title: J Nutr Sci Vitaminol (Tokyo) Volume: 57(6) Pages: 441-5
  • NAID: 40019136749
• [Journal Article] Extracts of common buckwheat bran prevent sucrose digestion. (2011)
  • Author(s): Hosaka T, Nii Y, Tomotake H, Ito T, Tamanaha A, Yamasaka Y, Sasaga S, Edazawa K, Tsutsumi R, Shuto E, Okahisa N, Iwata S, Sakai T.
  • Journal Title: J Nutr Sci Vitaminol (Tokyo) Volume: 57 Pages: 441-445
  • NAID: 40019136749
  • Peer Reviewed
• [Journal Article] Consumption of a resistant protein, sericin, elevates fecal immunoglobulin A, mucins, and cecal organic acids in rats fed a high-fat diet. (2011)
  • Author(s): Okazaki Y, Tomotake H, Tsujimoto K, Sasaki M, Kato N.
  • Journal Title: J Nutr Volume: 141 Pages: 1975-1981
  • Peer Reviewed
• [Presentation] 蚕サナギの 1-デオキシノジリマイシン含量および糖質分解酵素阻害作用 (2014)
  • Author(s): 友竹浩之, 片桐充昭
  • Organizer: 日本農芸化学会学術総会 2013年度大会
  • Place of Presentation: 明治大学
  • Year and Date: 2014-03-29
• [Presentation] 蚕サナギの1-デオキシノジリマイシン含量および糖質分解酵素阻害作用 (2014)
  • Author(s): 友竹浩之、片桐 充昭
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学
• [Presentation] ヤーコン粉末の特徴と利用 (2013)
  • Author(s): 友竹浩之, 千裕美
  • Organizer: 第60回日本栄養改善学会学術総会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2013-09-14
• [Presentation] 小麦アレルギー対応に向けた米粉およびホワイトソルガム粉の利用 (2013)
  • Author(s): 友竹浩之, 柴田あずさ, 長谷部遥, 花井希, 望月希代, 柴本むつ美
  • Organizer: 第67回日本栄養食糧学会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2013-05-26
• [Presentation] 蚕サナギおよびイナゴタンパク質の栄養特性 (2013)
  • Author(s): 友竹浩之, 片桐充昭, 柴本むつ美
  • Organizer: 日本農芸化学会学術総会 2013年度大会
  • Place of Presentation: 東北大学
  • Year and Date: 2013-03-27
• [Presentation] 小麦アレルギー対応に向けた米粉およびホワイトソルガム粉の利用 (2013)
  • Author(s): 友竹浩之、柴田あずさ、長谷部遥、花井希、望月希代、柴本 むつ美
  • Organizer: 第67回日本栄養食糧学会大会
  • Place of Presentation: 名古屋大学
• [Presentation] ヤーコン粉末の特徴と利用 (2013)
  • Author(s): 友竹 浩之、千裕美
  • Organizer: 第60回日本栄養改善学会
  • Place of Presentation: 神戸国際会議場
• [Presentation] 蚕サナギおよびイナゴタンパク質の栄養特性 (2013)
  • Author(s): 片桐 充昭、柴本 むつ美
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 東北大学
• [Presentation] 高圧加熱処理がそばタンパク質の溶解性と抗原性に及ぼす影響 (2012)
  • Author(s): 友竹浩之, 柴本むつ美, 富口由紀子
  • Organizer: 第59回日本栄養改善学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-09-13
• [Presentation] キクイモ粉末の特徴と利用 (2012)
  • Author(s): 友竹浩之, 牛山瑞穂, 園原一吉
  • Organizer: 第66回日本栄養食糧学会
  • Place of Presentation: 東北大学
  • Year and Date: 2012-05-20
• [Presentation] 高蛋白質そば粉抽出物の血糖上昇抑制作用 (2012)
  • Author(s): 友竹 浩之, 柴本 むつ美, 新居 佳孝
  • Organizer: 日本農芸化学会学術総会 2012年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-25
• [Presentation] キクイモ粉末の特徴と利用 (2012)
  • Author(s): 友竹浩之、牛山瑞穂, 園原一吉
  • Organizer: 第66回日本栄養食糧学会大会
  • Place of Presentation: 東北大学
• [Presentation] 高圧加熱処理がそば粉タンパク質の溶解性と抗原性に及ぼす影響 (2012)
  • Author(s): 友竹浩之、柴本むつ美、富口由紀子
  • Organizer: 第59回日本栄養改善学会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 黒ソバ粉が肥満・糖尿病マウスの血糖上昇に及ぼす影響 (2011)
  • Author(s): 友竹浩之, 柴本むつ美, 富口由紀子
  • Organizer: 第58回日本栄養改善学会学術総会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2011-09-09
• [Presentation] ポリフェノール摂取は高脂肪食摂取ラットの糞中ステロール組成を改善する (2011)
  • Author(s): 友竹浩之, Yunkyung Han, 原口智, 松本やよい, Yonghui Xu, 岡崎由佳子, 峰尾茂, 森山明穂, 井上淳詞, 加藤範久
  • Organizer: 第65回日本栄養食糧学会
  • Place of Presentation: お茶の水女子大学
  • Year and Date: 2011-05-15
• [Presentation] ポリフェノール摂取は高脂肪食摂取ラットの糞中ステロール組成を改善する
  • Author(s): 友竹浩之、Yunkyung Han, 原口智彰, 松本やよい, Yonghui Xu, 岡崎由佳子, 峰尾茂, 森山明穂, 井上淳詞, 加藤範久
  • Organizer: 第65回日本栄養・食糧学会大会
  • Place of Presentation: お茶の水女子大学
• [Presentation] 黒ソバ粉が肥満・糖尿病マウスの血糖上昇に及ぼす影響
  • Author(s): 友竹 浩之、柴本むつ美、富口由紀子
  • Organizer: 第58回日本栄養改善学会学術総会
  • Place of Presentation: 広島国際会議場
• [Presentation] 高蛋白質そば粉抽出物の血糖上昇抑制作用
  • Author(s): 友竹 浩之、柴本 むつ美、新居 佳孝
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学