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Structural analysis of sphingoglycolipid from Basidiomycota

Research Project

Project/Area Number 23500980
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Eating habits, studies on eating habits
Research InstitutionSoai University

Principal Investigator

MIZUNO seiko  相愛大学, 人間発達学部, その他 (90190652)

Co-Investigator(Kenkyū-buntansha) FUJIWARA Nagatoshi  帝塚山大学, 現代生活学部, 教授 (80326256)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsスフィンゴ糖脂質 / 生理活性 / グルコシルセラミド / 免疫賦活作用 / 糖脂質 / 化学分類
Research Abstract

In this study, we found out the synthesis of glycosphingolipids(GSL-1) in Maitake and Enokitake, and reported the structure in detail for the first time. The sugar component of GSL-1 was
assigned to be a glucose. LCB and FA fractions were analyzed by GC and GC/MS, and implied that the main components were C20:1 sphingenine for LCB, and C16:0, 2-OH-C16:0 for FA. Taken together, each GSL-1 of Maitake and Enokitake were same structure and defined as a glucosyl (1-1) ceramide. For biological clarification of the GSLs, the glycolipid fraction containing GSL-1 of Maitake and Enokitake were intraperitoneal injection to mouse. Numbers of peritoneal leukocyte were higher in mouse which intraperitoneal injected glycolipid fraction. From the results, the immunostimulatory effect of the glycolipid of Maitake and Enokitake was suggested.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (27 results)

All 2014 2013 2012 2011 Other

All Journal Article (13 results) (of which Peer Reviewed: 8 results) Presentation (13 results) Book (1 results)

  • [Journal Article] Glycerol monomycolate is a novel ligand for the human, but not mouse macrophage inducible C-type lectin, mincle2014

    • Author(s)
      Hattori Y, D. Morita, N. Fujiwara, D. Mori, T. Nakamura, H. Harashima, S. Yamasaki, and M. Sugita
    • Journal Title

      J. Biol. Chem. J Biol Chem

      Volume: 289(22) Pages: 15405-15412

    • Related Report
      2013 Final Research Report
  • [Journal Article] ハンビラタケ子実体および脂質成分の免疫賦活作用2014

    • Author(s)
      庄條愛子、古豪加奈、山本玲奈、小林貴美子、藤原永年、水野淨子
    • Journal Title

      相愛大学研究論集

      Volume: 30 Pages: 15-20

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial sphingo phospholipids containing non fatty acid activate murine macrophages via Toll-like receptor 4 and bacterial clearance2013

    • Author(s)
      Fujiwara, N., S.A. Porcelli, T. Naka, I. Yano, S. Maeda, H. Kuwata, S. Akira, S. Uematsu, T. Takii, H. Ogura, and K. Kobayashi
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1831 (6) Pages: 1177-1184

    • Related Report
      2013 Final Research Report
  • [Journal Article] The mannan antigen lacking β-1,2-linked oligomannosides from Candida albicans increases production of inflammatory cytokines released by dendritic cells2013

    • Author(s)
      Ueno, K.
    • Journal Title

      Med. Microbiol.

      Volume: 51 Issue: 4 Pages: 385-395

    • DOI

      10.3109/13693786.2012.733892

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bacterial sphingophospholipids containing non-hydroxy fatty acid activate murine macrophages via Toll-like receptor 4 and stimulate bacterial clearance2013

    • Author(s)
      Fujiwara, N.
    • Journal Title

      Biochim. Biophys. Acta.

      Volume: Mar 29 Issue: 6 Pages: 1177-84

    • DOI

      10.1016/j.bbalip.2013.03.008

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Antitubercular activity of disulfiram, an antialcoholism drug, against multidrug- and extensively drug-resistant Mycobacterium tuberculosis isolates.2012

    • Author(s)
      Horita Y, Takii T, Yagi T, Ogawa K, Fujiwara N, Inagaki E, Kremer L, Sato Y, Kuroishi R, Lee Y, Makino T, Mizukami H, Hasegawa T, Yamamoto R, Onozaki K.
    • Journal Title

      Antimicrob Agents Chemother.

      Volume: 56(8) Issue: 8 Pages: 4140-5

    • DOI

      10.1128/aac.06445-11

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The polyketide synthase-associated multidrug tolerance in Mycobacterium intracellulare clinical isolates2012

    • Author(s)
      Matsunaga, I.
    • Journal Title

      Chemotherapy

      Volume: 58 Issue: 5 Pages: 341-348

    • DOI

      10.1159/000343311

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 担子菌由来スフィンゴ糖脂質の構造解析2012

    • Author(s)
      小林貴美子、合田麗奈、水野淨子、中崇、土江松美、前田伸司、藤原永年
    • Journal Title

      相愛大学研究論集

      Volume: 28 Pages: 203-213

    • NAID

      120005570980

    • Related Report
      2011 Research-status Report
  • [Journal Article] Characteristics of Mycobacterium smegmatis J15cs strain lipids2012

    • Author(s)
      N. Fujiwara, T. Naka, M. Ogawa, R. Yamamoto, H. Ogura, and H. Taniguchi
    • Journal Title

      Tuberculosis (Edinb)

      Volume: 92 Pages: 187-192

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structure and host recognition of serotype 13 glycopeptidolipid from Mycobacterium intracellulare2011

    • Author(s)
      T. Naka, N. Nakata, S. Maeda, R. Yamamoto, M. Doe, S. Mizuno, M. Niki, K. Kobayasi, H. Ogura, M. Makino, and N. Fujiwara
    • Journal Title

      Journal of Bacteriology

      Volume: 193 Pages: 5766-5774

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The lipid phenotype of two distinct subpopulations of Mycobacterium bovis Bacillus Calmette-Guerin Tokyo 172 substrain2011

    • Author(s)
      T. Naka, S. Maeda, M. Niki, N. Ohara, S. Yamamoto, I. Yano, J. Maeyama, H. Ogura, K. Kobayashi, and N. Fujiwara
    • Journal Title

      Journal of Biological Chemistry

      Volume: 286 Pages: 44153-44161

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] ハナビラタケ子実体および脂質成分の免疫賦活作用

    • Author(s)
      庄條愛子, 古豪加奈, 山本麗奈, 小林貴美子, 中崇, 藤原永年, 北村進一, 水野淨子
    • Journal Title

      相愛大学研究論集

      Volume: 第 30 巻

    • Related Report
      2013 Final Research Report
  • [Journal Article] Structural analysis of sphingoglycolipid from Basidiomycota

    • Author(s)
      Kiniko Kobayasi, Reina Goda, Seiko Mizuno, Takashi Naka, Shinji Maeda and Nagatoshi Fujiwara
    • Journal Title

      相愛大学研究論集

      Volume: 第28 巻

    • NAID

      120005570980

    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular composition of the intact glycopeptidolipid in cell-infected Mycobacterium intra- cellulare2014

    • Author(s)
      Naka, T., S. Maeda, M. Shibata, S. Mizuno, N. Fujiwara
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京(タワーホール船堀)
    • Related Report
      2013 Final Research Report
  • [Presentation] Rapid analysis of mycobacterial mycolate by MALDI spiral-TOFMS with ultra high mass-resolving power2014

    • Author(s)
      Teramoto, K., N. Fujiwara
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京(タワーホール船堀)
    • Related Report
      2013 Final Research Report
  • [Presentation] Induction of arcA mRNA by an intracellular acidification in Mycobacterium avium2014

    • Author(s)
      Ogawa S., K. Ogawa, T. Yagi, N. Ohara, Y. Goto, N. Fujiwara, S. Maeda, T. Yamazaki, S. Itoh, T. Takii
    • Organizer
      第87回日本細菌学会総会
    • Place of Presentation
      東京(タワーホール船堀)
    • Related Report
      2013 Final Research Report
  • [Presentation] Molecular composition of the intact glycopeptidolipid in cell-infected Mycobacterium intracellulare.2014

    • Author(s)
      Naka, T., S. Maeda, M. Shibata, S. Mizuno, N. Fujiwara.
    • Organizer
      第87回日本細菌学会
    • Place of Presentation
      東京(タワーホール船堀)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Bacterial sphingophospho- lipids activate murine macrophages via Toll-like receptor 4 and stimulate bacterial clearance2013

    • Author(s)
      N. Fujiwara, T. Naka, H. Kuwata, S. Maeda
    • Organizer
      FEMS 2013-The 5th Congress of European Microbiologists
    • Place of Presentation
      Reipzig, Germany
    • Related Report
      2013 Final Research Report
  • [Presentation] Application of MALDI Spiral-TOF MS with High Mass Resolving Power to Assign Mycolic Acid Subclasses in Mycobacterium tuber- culosis2013

    • Author(s)
      K. Teramoto, T. Sato, T. Wada, A. Yamamoto, N. Fujiwara
    • Organizer
      FEMS 2013-The 5th Congress of European Microbiologists
    • Place of Presentation
      Reipzig, Germany
    • Related Report
      2013 Final Research Report
  • [Presentation] Novel serotype-specific glycopeptidolipid from Mycobac-terium intracellulare clinical isolate2013

    • Author(s)
      N. Fujiwara, T. Naka, M. Ayata, M. Shibata, H. Kuwata, H. Ogura, and S. Maeda
    • Organizer
      The 5th EMBO meeting
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2013 Final Research Report
  • [Presentation] Genotyping of Mycobacterium tuberculosis (MTB), especially Beijing genotype MTB, in Japan by using the variable-number of tandem repeats (VNTR) and a next generation sequencer (NGS)2013

    • Author(s)
      S. Maeda, T. Wada , T. Naka , M. Shibata , N. Fujiwara
    • Organizer
      The 5th EMBO meeting
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2013 Final Research Report
  • [Presentation] Production of anti-lipid IgG antibodies in rabbit immunized with mycobacterial lipid antigens2013

    • Author(s)
      M. Shibata, T. Naka, S. Maeda, and N. Fujiwara
    • Organizer
      The 5th EMBO meeting
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2013 Final Research Report
  • [Presentation] ハナビラタケおよび脂質成分の免疫賦活作用の検討2012

    • Author(s)
      庄條愛子, 水野淨子, 古豪加奈, 小林貴美子, 合田麗奈, 中崇, 藤原永年
    • Organizer
      2012年度 日本農芸化学会関西支部会
    • Place of Presentation
      京都(京都学園大学)
    • Year and Date
      2012-09-29
    • Related Report
      2013 Final Research Report
  • [Presentation] 臨床分離株MAC菌由来新規血清型特異糖ペプチド脂質抗原と宿主応答2012

    • Author(s)
      中崇、佐藤明正、前田伸司、柴田満、水野淨子、藤原永年
    • Organizer
      第85日本細菌学会
    • Place of Presentation
      長崎
    • Related Report
      2011 Research-status Report
  • [Presentation] 臨床分離MAC菌の血清型特異糖ペプチド脂質抗原の分布と新規構造2011

    • Author(s)
      藤原永年、合田麗奈、佐藤明正、矢野郁也、前田伸司
    • Organizer
      第86回日本結核病学会総会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] ハナビラタケおよび脂質成分の免疫賦活作用の検討

    • Author(s)
      庄條愛子、古郷加奈、小林貴美子、藤原永年、北村進一、水野 淨子
    • Organizer
      日本農芸化学会 関西支部大会
    • Place of Presentation
      亀岡市
    • Related Report
      2012 Research-status Report
  • [Book] Anticancer immunotherapeutics: mycoloyl glycolipids and lipoglycan in the cell wall, In T. Takii, J. Maeyama, S. Yamamoto (ed.), BCG-Vaccine and Adjuvant.2011

    • Author(s)
      N. Fujiwara, and I. Yano
    • Publisher
      Japan Anti-Tuberculosis Association, Tokyo
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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