Involvement of DNA recombination repair proteins in chromosomal translocations
| Project/Area Number |
23501259
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Carcinogenesis
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| Research Institution | Hiroshima University |
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Principal Investigator |
SUN Jiying 広島大学, 原爆放射線医科学研究所, 講師 (80397926)
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| Co-Investigator(Kenkyū-buntansha) |
TASHIRO Satoshi 広島大学, 原爆放射線医科学研究所, 教授 (20243610)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 染色体転座 / ATM / 組換え修復 / RAD51 / RPA / DNA組換え修復 / RPA2 |
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| Research Abstract |
Chromosome translocations induced by ionizing radiation and chemotherapeutic agents, has been shown to lead to malignant transformation. However, the mechanism of chromosome translocations is still unclear. Chromosome translocations involving the MLL gene on 11q23 are the most frequent chromosome abnormalities in secondary leukemias associated with chemotherapy employing etoposide. Dysfunction of ATM, a DNA damage signaling regulator, increases the incidence of 11q23 chromosome translocations. We showed that ATM deficiency results in the excessive binding of the DNA recombinase RAD51 at the translocation breakpoint cluster region (BCR) of MLL gene after etoposide exposure. In this study, we showed that phosphorylation of RPA2 by ATM plays an important role in the regulation of RAD51 binding to the BCR of MLL gene after etoposide exposure.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Activation of the SUMO modification system is required for the accumulation of RAD51 at sites containing DNA damage2013
Author(s)
Shima H, Suzuki H, Sun J, Kono K, Shi L, Kinomura A, Horikoshi Y, Ikura T, Ikura M, Kanaar R, Igarashi K, Saitoh H, Kurumizaka H, Tashiro S
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Journal Title
J Cell Sci
Volume: 126
Pages: 5284-5292
DOI
Related Report
Peer Reviewed
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[Journal Article] Involvement of homologous recombination in the synergism between cisplatin and poly(ADP-ribose) polymerase inhibition2013
Author(s)
Sakogawa K, Aoki Y, Misumi K, Hamai Y, Emi M, Hihara J, Shi L, Kono K, Horikoshi Y, Sun J, Ikura T, Okada M, Tashiro S
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Journal Title
Cancer Sci. 2013 Sep 5
Volume: 104
Issue: 12
Pages: 1593-1609
DOI
Related Report
Peer Reviewed
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[Journal Article] Involvement of ribonucleotide reductase-M1 in 5-fluorouracil induced DNA damage in esophageal cancer cell lines.2013
Author(s)
Aoki Y, Sakogawa K, Hihara J, Emi M, Hamai Y, Kono K, Shi L, Sun J, Kitao H, Ikura T, Niida H, Nakanishi M, Okada M, Tashiro S.
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Journal Title
Int J Oncol.
Volume: 42(6)
Issue: 6
Pages: 1951-60
DOI
Related Report
Peer Reviewed
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[Journal Article] A Modified System for Analyzing Ionizing Radiation-Induced Chromosome Abnormalities2012
Author(s)
Shi L, Fujioka K, Sun J, Kinomura A, Inaba T, Ikura T, Ohtaki M, Yoshida M, Kodama Y, Livingston GK, Kamiya K, Tashiro S
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Journal Title
Radiat Res
Volume: 177(5)
Pages: 533-538
URL
Related Report
Peer Reviewed
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[Journal Article] A modified system for analyzing ionizing radiation-induced chromosome abnormalities.2012
Author(s)
Shi L, Fujioka K, Sun J, Kinomura A, Inaba T, Ikura T, Ohtaki M, Yoshida M, Kodama Y, Livingston GK, Kamiya K, Tashiro S.
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Journal Title
Radiat Res.
Volume: 177
Pages: 533-8
Related Report
Peer Reviewed
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