Investigation of RNA binding proteins that specifically suppress a miRNA maturation in tumor cells.

• Project/Area Number: 23501264
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Tohoku University
• Principal Investigator: YASUDA Jun 東北大学, 東北メディカル・メガバンク機構, 教授 (00281684)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: マイクロRNA / 発がん / RNA結合タンパク質

Research Abstract

I have identified a RNA binding protein which specifically binds to a miRNA precursor in medulloblastoma cells. Suppression of the protein by the use of siRNAs causes the increase of the mature miRNA whose precursor binds to the target protein. I also test if the downstream target genes of the increased miRNA are suppressed by the siRNA treatment for the RNA binding protein. Finally, I analyzed the genomic sequences of the RNA binding proteins in the human tumor cells to test the relationship between the RNA binding proteins and tumorigenesis in human tumor cell lines.

Research Products

• [Journal Article] Inference of Gene Regulation via miRNAs During ES Cell Differentiation Using MiRaGE Method. (2011)
  • Author(s): Yoshizawa, M., Taguchi, Y_h, Yasuda, J.
  • Journal Title: International Journal of Molecular Sciences Volume: 12 Pages: 9265-9271
  • Peer Reviewed
• [Presentation] Erb3-binding protein 1 (Ebp1) may suppress miR-124 maturation in the Medulloblastoma of the Ptc1 Heterozygous Mice (2012)
  • Author(s): J.Yasuda, K.Yaginuma, Y.Saiki, Y.Sugitani, C.Obuse, T.Noda
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 2012-09-21
• [Presentation] EBP1はPtc1ヘテロマウスに発症する髄芽腫においてmiR-124の成熟過程を阻害する (2012)
  • Author(s): 安田純、柳沼克幸、齋木由利子、杉谷善信、小布施力史、野田哲生
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 北海道札幌市
• [Presentation] The cloning and characterization of specific modifiers of miRNA maturation in the medulloblastoma of the ptc1 heterozygous mice (2011)
  • Author(s): J.Yasuda, K.Yaginuma, Y.Saiki, Y.Sugitani, C.Obuse, T.Noda
  • Organizer: CELL Symposia for Regulatory RNAs 2011
  • Place of Presentation: Chicago
  • Year and Date: 2011-10-11
• [Presentation] The Cloning and Characterization of Specific Modifiers of miRNA Maturation Suppressed in the Medulloblastoma of the Ptc1 Heterozygous Mice. (2011)
  • Author(s): Jun Yasuda, Katsuyuki Yaginuma, Yuriko Saiki, Yoshinobu Sugitani, Chikashi Obuse, Tetsuo Noda
  • Organizer: RNA 2011
  • Place of Presentation: 京都国際会館
• [Presentation] EBP1 SUPPRESSES MATURATION OF THE TUMOR SUPPRESSIVE MIR-124 IN THE MEDULLOBLASTOMA OF THE PTCH1 HETEROZYGOUS MICE. (2011)
  • Author(s): Jun Yasuda Katsuyuki Yaginuma Yuriko Saiki Yoshinobu Sugitani Chikashi Obus Tetsuo Noda
  • Organizer: The 16th Korea-Japan Cancer Research Workshop
  • Place of Presentation: 北海道大学