Search for RASSF-interacting molecules using C. elegans
| Project/Area Number |
23501272
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Hideki 立命館大学, 生命科学部, 助教 (20550156)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | がん細胞の特性 / チェックポイント / RASSF / がん抑制遺伝子 / DNA損傷修復 / RIF1 / 線虫 / Rasf-1 / Rab-39 / Rif1 / Rassf1A / DNA合成阻害 |
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| Research Abstract |
We screened molecules interacting with C. elegans RASSF homolog, T24F1.3 (we named as rasf-1), by yeast two hybrid system and obtained rab-39 and F11E6.7. We named F11E6.7 as rif-1 because it is phylogenetically close to rif1. Transient expression in HEK293T cells and immunoprecipitation revealed that both mammalian homologs Rab39 and Rif1 bind to RASSF1A. As to the functions of Rab-39 and Rif-1, we demonstrated that Rasf-1 and Rab-39 function in the same signaling pathway in response to oxidative stress and that Rif-1 plays an important role in DNA damage repair as mammalian Rif1.
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Report
(4 results)
Research Products
(29 results)
