molecular mechanisms of Ink4a/Arf locus that cofers cell aging and differentiation

• Project/Area Number: 23501277
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: NAKADE koji 独立行政法人理化学研究所, バイオリソースセンター, 協力研究員 (20392053)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 細胞分化 / iPS / 心筋細胞 / アデノウイルスベクター / エピジェネティックス / ヒストン / クロマチン / メチル化 / エピジェネシス / 分化 / 脱分化 / 老化 / アデノウイルス

Research Abstract

We have analyzed molecular mechanisms of JDP2 in the epigenetic regulation of Ink4a locus and found that JDP2 helps maintaining non-methylation status of histones by concerting with histone demethylase, Jmjd3, not only by inhibiting the activity of methytransferases, PRC1 and PRC2. In addition, in order to analyze the effect of JDP2 on differentiation and de-differentiation, we established, as a model, adenoviral gene introduction system that successfully induced cardiomyocyte and iPS from mouse embryo fibroblast. Using these system, we obtained the result that the inducibility of diffrentiation and de-diffrentiation was improved in the absence of JDP2.

Research Products

• [Journal Article] Cyclophosphamide enhances antitumor efficacy of oncolytic adenovirus expressing uracil phosphoribosyltransferase (UPRT) in immunocompetent Syrian hamsters (2013)
  • Author(s): Hasegawa N, Abei M, Yokoyama KK, Fukuda K, Seo E, Kawashima R, Nakano Y, Yamada T, Nakade K, Hamada H, Obata Y, Hyodo I
  • Journal Title: Int. J.Cancer Volume: PMID: 23444104(印刷中) Issue: 6 Pages: 1479-1488
  • DOI: 10.1002/ijc.28132
  • Peer Reviewed
• [Journal Article] Induction of Cardiomyocyte-like Cells in Infarct Hearts by Gene Transfer of Gata4, Mef2c, and Tbx5 (2012)
  • Author(s): Inagawa K, Miyamoto K, Yamakawa H, Muraoka N, Sadahiro T, Umei T, Wada R, Katsumata Y, Kaneda R, Nakade K, Kurihara C, Obata Y, Miyake K, Fukuda K, Ieda M
  • Journal Title: Circulation research Volume: 111 Issue: 9 Pages: 1147-56
  • DOI: 10.1161/circresaha.112.271148
  • Peer Reviewed
• [Presentation] 複数因子同時発現アデノウイルスベクターを用いた直接心筋分化誘導系の確立 (2013)
  • Author(s): 中出浩司、栗原千登勢、村田武英、小幡裕一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-04
• [Presentation] 組換えアデノウイルスバンクの概要と2A 自己開裂ペプチドを介したGFP 共発現組換えアデノウイルスの開発 (2013)
  • Author(s): 栗原千登勢、中出浩司、村田武英、小幡裕一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-03
• [Presentation] 複数因子同時発現アデノウイルスベクターを用いた直接心筋分化誘導系の確立 (2013)
  • Author(s): 中出浩司、栗原千登勢、村田武英、小幡裕一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 組換えアデノウイルスバンクの概要と2A自己開裂ペプチドを介したGFP共発現組換えアデノウイルスの開発 (2013)
  • Author(s): 栗原千登勢、中出浩司、村田武英、小幡裕一
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Animal and Microbial DNA Resources Available from RIKEN BioResource Center (2013)
  • Author(s): 8. Murata, T., Kishikawa, S., Kujime, Y., Kurihara, C., Masuzaki, S., Nakade, K., Nakamura, N., Nakashima, K., Okubo, M., Sakayori, M., Sasanuma, S., Shibamoto, Y., Yamasaki, T. and Obata
  • Organizer: The 5th ANRRC (Asian Network of Research Resource Centers) International Meeting
  • Place of Presentation: 葉山湘南国際村センター
• [Presentation] Induction of cardiomyocites mediated by Adenoviral vector (2012)
  • Author(s): 中出浩司、栗原千登勢、村田武英、小幡裕一
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] 組換えアデノウイルスバンクの開発 (2012)
  • Author(s): 栗原千登勢、中出浩司、村田武英、小幡裕一
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] アデノウイルスバンクの開発 (2011)
  • Author(s): 中出浩司、栗原千登勢、村田武英、小幡裕一
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] 組換えアデノウイルスバンクの開発 (2011)
  • Author(s): 中出浩司、栗原千登勢、村田武英、小幡裕一
  • Organizer: 日本ケミカルバイオロジー学会 第6回年会
  • Place of Presentation: 東京工業大学 大岡山キャンパス
• [Presentation] The development of recombinant virus bank (2011)
  • Author(s): Koji Nakade, Chitose Kurihara, Takehide, Murata, Yuichi,Obata
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜