Analysis of Sp1-dependent adaptation mechanism of cancer cells to hypoxia for tumor microenvironment-targeted therapy
| Project/Area Number |
23501280
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Kanagawa Cancer Center Research Institute |
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Principal Investigator |
KOIZUME Shiro 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), その他部局等, その他 (60416063)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAGI Yohei 神奈川県立がんセンター臨床研究所, 部長 (00254194)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ハイポキシア / 卵巣癌 / 遺伝子発現 |
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| Research Abstract |
Accumulating evidences have suggested that tumor microenvironment is an attractive target of cancer therapy. However, there are few effective strategies to accomplish it. We previously showed that FVII gene is synergistically induced under serum staved hypoxia in ovarian cancer cells. Mechnism of this transcriptional upregulation is associates with HIF2-Sp1 interaction and distinct from that of authentic hypoxia responsive genes such as VEGF. In this study, we investigated detailed mechanism of this novel transcriptional regulation. Genes regulated by similar Sp1 dependent mechanism and their function were further explored by various experiments. We found that ICAM-1 protein is robustly induced by this mechanism and contributes to growth accerelation of ovarian clear cell carcinoma cells.
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Report
(4 results)
Research Products
(58 results)
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[Journal Article] Annexin A4 is involved in proliferation, chemoresistance and migration and invasion in ovarian clear cell adenocarcinoma cells.2013
Author(s)
Mogami T, Yokota N, Asai-sato M, Yamada R, Koizume S, Sakuma Y, Yoshihara M, Nakamura Y, Takano Y, Hirahara F, Miyagi Y, and Miyagi E.
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Journal Title
PLOS ONE
Volume: 8
Issue: 11
Pages: 1-8
DOI
Related Report
Peer Reviewed
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[Journal Article] Enhanced autophagy is required for survival in EGFR-independent EGFR-mutant lung adenocarcinoma cells.2013
Author(s)
Sakuma Y, Matsukuma S, Nakamura Y, Yoshihara M, Koizume S, Sekiguchi H, Saito H, Nakayama H, Kameda Y, Yokose T, Oguni S, Niki T, and Miyagi Y.
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Journal Title
Lab Invest.
Volume: 93
Issue: 10
Pages: 1137-1146
DOI
Related Report
Peer Reviewed
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[Journal Article] The association between RAS gene mutations and clinical characteristics in follicular thyroid tumors: new insights from a single center and a large patient cohort2012
Author(s)
Fukahori M, Yoshida A, Hayashi H, Yoshihara M, Matsukuma S, Sakuma Y, Koizume S, Okamoto N, Kondo T, Masuda M, and Miyagi Y
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Journal Title
Thyroid
Volume: 22
Issue: 7
Pages: 683-689
DOI
Related Report
Peer Reviewed
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[Journal Article] Self-production of tissue factor-coagulation factor VII complex by ovarian cancer cells2009
Author(s)
Yokota, N., Koizume, S., Miyagi, E., Hirahara, F., Nakamura, Y., Kikuchi, K., Ruf, W., Sakuma, Y. Tsuchiya, E., and Miyagi, Y
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Journal Title
Br. J. Cancer
Volume: 101
Issue: 12
Pages: 2023-2029
DOI
Related Report
Peer Reviewed
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[Journal Article] HNF-4-independent synthesis of coagulation factor VII in breast cancer cells and its inhibition by targeting selective histone acetyltransferase2009
Author(s)
Koizume, S., Yokota, N., Miyagi, E., Hirahara, F., Nakamura, Y., Sakuma, Y., Yoshida, A., Kameda, Y., Tsuchiya, E. and Miyagi, Y
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Journal Title
Mol. Cancer Res.
Volume: 7
Issue: 12
Pages: 1928-1936
DOI
Related Report
Peer Reviewed
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[Presentation] Functional analysis of annexin A4 and the implication in ovarian clear cell carcinoma (OCCA) phenotypes2012
Author(s)
Tae Mogami, Naho Yokota, Mitsuyo Yoshihara, Yoshiyasu Nakamura, Yuji Sakuma, Shiro Koizume, Mikiko Asai-Sato, Etsuko Miyagi, Fumiki Hirahara, and Yohei Miyagi
Organizer
The 103^<th> AACR annual meeting
Place of Presentation
Chicago, IL
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