| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
We have previously established a 2-step culture method for differentiating Foxp3- naive CD4+ T cells to Foxp3+ induced-regulatory T cells (iTregs). Firstly, we stimulate naive CD4+ T cells by anti-CD3 mAb and anti-CD28 mAb in the presence of Jak3 inhibitor for 3 days (conditioning stage). Secondary, we keep culturing Foxp3- iTregs precursor T cells to differentiate these cells to Foxp3+ iTregs in the presence of IL-2 for 3 days (differentiation stage). We analyzed what kind of factors could affect this protocol. In this study, we have identified that IL-2 signaling pathway was the main target of Jak3 inhibitor, and IL-4 was the strongest inhibiting cytokine in the differentiation stage from Foxp3- iTregs precursor cells to Foxp3+ iTregs. We also confirmed that STAT6, a downstream molecule of IL-4 signaling pathway, was an important molecule. These results obtained in this study are important for developing effective cancer treatment by regulating the differentiation into iTregs.
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